The occurrence of specific markers of Bacteroides fragilis group, B. dorei and antibiotic-resistance genes in the wastewater treatment plants
Wastewater treatment plants (WWTPs) are one of the main transmission sources of pathogenic bacteria and antibiotic-resistance genes in the natural environment. In this study, the presence of specific markers of Bacteroides fragilis group (BFG), B. dorei and genes encoding resistance to beta-lactams...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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EDP Sciences
2018-01-01
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| Series: | E3S Web of Conferences |
| Online Access: | https://doi.org/10.1051/e3sconf/20184400124 |
| _version_ | 1818918086442483712 |
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| author | Niestępski Sebastian Harnisz Monika Korzeniewska Ewa Osińska Adriana |
| author_facet | Niestępski Sebastian Harnisz Monika Korzeniewska Ewa Osińska Adriana |
| author_sort | Niestępski Sebastian |
| collection | DOAJ |
| description | Wastewater treatment plants (WWTPs) are one of the main transmission sources of pathogenic bacteria and antibiotic-resistance genes in the natural environment. In this study, the presence of specific markers of Bacteroides fragilis group (BFG), B. dorei and genes encoding resistance to beta-lactams (cepA, cfxA), tetracyclines (tet(Q)), macrolides, lincosamides and streptogramins (MLS) mechanism (ermF, linA) was analyzed by standard PCR in the inflows and outflows from three wastewater treatment plants with the activated sludge process. Genetic material was isolated from wastewater samples with the use of two commercial kits for genomic DNA extraction, the Fast DNA SPIN Kit for Soil and the Genomic Micro AX Bacteria Gravity Kit. The quality of the isolated genetic material differed between the tested isolation kits. The Fast DNA SPIN Kit for Soil was more effective in detecting cfxA, ermF and linA genes. However, both extraction kits effectively identified tet(Q), bfr and HF183/BacR287 genes in all wastewater samples. The results of the study indicate that genes specific to BFG and B. dorei, and genes encoding resistance to MLS and tetracyclines are not completely eliminated during the wastewater treatment process. |
| first_indexed | 2024-12-20T00:44:22Z |
| format | Article |
| id | doaj.art-94787a87344c4a8db14803e43c74e199 |
| institution | Directory Open Access Journal |
| issn | 2267-1242 |
| language | English |
| last_indexed | 2024-12-20T00:44:22Z |
| publishDate | 2018-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | E3S Web of Conferences |
| spelling | doaj.art-94787a87344c4a8db14803e43c74e1992022-12-21T19:59:26ZengEDP SciencesE3S Web of Conferences2267-12422018-01-01440012410.1051/e3sconf/20184400124e3sconf_eko-dok2018_00124The occurrence of specific markers of Bacteroides fragilis group, B. dorei and antibiotic-resistance genes in the wastewater treatment plantsNiestępski SebastianHarnisz MonikaKorzeniewska EwaOsińska AdrianaWastewater treatment plants (WWTPs) are one of the main transmission sources of pathogenic bacteria and antibiotic-resistance genes in the natural environment. In this study, the presence of specific markers of Bacteroides fragilis group (BFG), B. dorei and genes encoding resistance to beta-lactams (cepA, cfxA), tetracyclines (tet(Q)), macrolides, lincosamides and streptogramins (MLS) mechanism (ermF, linA) was analyzed by standard PCR in the inflows and outflows from three wastewater treatment plants with the activated sludge process. Genetic material was isolated from wastewater samples with the use of two commercial kits for genomic DNA extraction, the Fast DNA SPIN Kit for Soil and the Genomic Micro AX Bacteria Gravity Kit. The quality of the isolated genetic material differed between the tested isolation kits. The Fast DNA SPIN Kit for Soil was more effective in detecting cfxA, ermF and linA genes. However, both extraction kits effectively identified tet(Q), bfr and HF183/BacR287 genes in all wastewater samples. The results of the study indicate that genes specific to BFG and B. dorei, and genes encoding resistance to MLS and tetracyclines are not completely eliminated during the wastewater treatment process.https://doi.org/10.1051/e3sconf/20184400124 |
| spellingShingle | Niestępski Sebastian Harnisz Monika Korzeniewska Ewa Osińska Adriana The occurrence of specific markers of Bacteroides fragilis group, B. dorei and antibiotic-resistance genes in the wastewater treatment plants E3S Web of Conferences |
| title | The occurrence of specific markers of Bacteroides fragilis group, B. dorei and antibiotic-resistance genes in the wastewater treatment plants |
| title_full | The occurrence of specific markers of Bacteroides fragilis group, B. dorei and antibiotic-resistance genes in the wastewater treatment plants |
| title_fullStr | The occurrence of specific markers of Bacteroides fragilis group, B. dorei and antibiotic-resistance genes in the wastewater treatment plants |
| title_full_unstemmed | The occurrence of specific markers of Bacteroides fragilis group, B. dorei and antibiotic-resistance genes in the wastewater treatment plants |
| title_short | The occurrence of specific markers of Bacteroides fragilis group, B. dorei and antibiotic-resistance genes in the wastewater treatment plants |
| title_sort | occurrence of specific markers of bacteroides fragilis group b dorei and antibiotic resistance genes in the wastewater treatment plants |
| url | https://doi.org/10.1051/e3sconf/20184400124 |
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