ROS-producing immature neutrophils in giant cell arteritis are linked to vascular pathologies
Giant cell arteritis (GCA) is a common form of primary systemic vasculitis in adults, with no reliable indicators of prognosis or treatment responses. We used single cell technologies to comprehensively map immune cell populations in the blood of patients with GCA and identified the CD66b+CD15+CD10l...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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American Society for Clinical investigation
2020-10-01
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| Series: | JCI Insight |
| Subjects: | |
| Online Access: | https://doi.org/10.1172/jci.insight.139163 |
| _version_ | 1818723000077254656 |
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| author | Lihui Wang Zhichao Ai Tariq Khoyratty Kristina Zec Hayley L. Eames Erinke van Grinsven Alison Hudak Susan Morris David Ahern Claudia Monaco Evgeniy B. Eruslanov Raashid Luqmani Irina A. Udalova |
| author_facet | Lihui Wang Zhichao Ai Tariq Khoyratty Kristina Zec Hayley L. Eames Erinke van Grinsven Alison Hudak Susan Morris David Ahern Claudia Monaco Evgeniy B. Eruslanov Raashid Luqmani Irina A. Udalova |
| author_sort | Lihui Wang |
| collection | DOAJ |
| description | Giant cell arteritis (GCA) is a common form of primary systemic vasculitis in adults, with no reliable indicators of prognosis or treatment responses. We used single cell technologies to comprehensively map immune cell populations in the blood of patients with GCA and identified the CD66b+CD15+CD10lo/–CD64– band neutrophils and CD66bhiCD15+CD10lo/–CD64+/bright myelocytes/metamyelocytes to be unequivocally associated with both the clinical phenotype and response to treatment. Immature neutrophils were resistant to apoptosis, remained in the vasculature for a prolonged period of time, interacted with platelets, and extravasated into the tissue surrounding the temporal arteries of patients with GCA. We discovered that immature neutrophils generated high levels of extracellular reactive oxygen species, leading to enhanced protein oxidation and permeability of endothelial barrier in an in vitro coculture system. The same populations were also detected in other systemic vasculitides. These findings link functions of immature neutrophils to disease pathogenesis, establishing a clinical cellular signature of GCA and suggesting different therapeutic approaches in systemic vascular inflammation. |
| first_indexed | 2024-12-17T21:03:33Z |
| format | Article |
| id | doaj.art-9479450c622941bc9ee438a4e6c35b4b |
| institution | Directory Open Access Journal |
| issn | 2379-3708 |
| language | English |
| last_indexed | 2024-12-17T21:03:33Z |
| publishDate | 2020-10-01 |
| publisher | American Society for Clinical investigation |
| record_format | Article |
| series | JCI Insight |
| spelling | doaj.art-9479450c622941bc9ee438a4e6c35b4b2022-12-21T21:32:39ZengAmerican Society for Clinical investigationJCI Insight2379-37082020-10-01520ROS-producing immature neutrophils in giant cell arteritis are linked to vascular pathologiesLihui WangZhichao AiTariq KhoyrattyKristina ZecHayley L. EamesErinke van GrinsvenAlison HudakSusan MorrisDavid AhernClaudia MonacoEvgeniy B. EruslanovRaashid LuqmaniIrina A. UdalovaGiant cell arteritis (GCA) is a common form of primary systemic vasculitis in adults, with no reliable indicators of prognosis or treatment responses. We used single cell technologies to comprehensively map immune cell populations in the blood of patients with GCA and identified the CD66b+CD15+CD10lo/–CD64– band neutrophils and CD66bhiCD15+CD10lo/–CD64+/bright myelocytes/metamyelocytes to be unequivocally associated with both the clinical phenotype and response to treatment. Immature neutrophils were resistant to apoptosis, remained in the vasculature for a prolonged period of time, interacted with platelets, and extravasated into the tissue surrounding the temporal arteries of patients with GCA. We discovered that immature neutrophils generated high levels of extracellular reactive oxygen species, leading to enhanced protein oxidation and permeability of endothelial barrier in an in vitro coculture system. The same populations were also detected in other systemic vasculitides. These findings link functions of immature neutrophils to disease pathogenesis, establishing a clinical cellular signature of GCA and suggesting different therapeutic approaches in systemic vascular inflammation.https://doi.org/10.1172/jci.insight.139163Vascular biology |
| spellingShingle | Lihui Wang Zhichao Ai Tariq Khoyratty Kristina Zec Hayley L. Eames Erinke van Grinsven Alison Hudak Susan Morris David Ahern Claudia Monaco Evgeniy B. Eruslanov Raashid Luqmani Irina A. Udalova ROS-producing immature neutrophils in giant cell arteritis are linked to vascular pathologies JCI Insight Vascular biology |
| title | ROS-producing immature neutrophils in giant cell arteritis are linked to vascular pathologies |
| title_full | ROS-producing immature neutrophils in giant cell arteritis are linked to vascular pathologies |
| title_fullStr | ROS-producing immature neutrophils in giant cell arteritis are linked to vascular pathologies |
| title_full_unstemmed | ROS-producing immature neutrophils in giant cell arteritis are linked to vascular pathologies |
| title_short | ROS-producing immature neutrophils in giant cell arteritis are linked to vascular pathologies |
| title_sort | ros producing immature neutrophils in giant cell arteritis are linked to vascular pathologies |
| topic | Vascular biology |
| url | https://doi.org/10.1172/jci.insight.139163 |
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