Crystal structure of Drosophila Piwi
PIWI-clade Argonautes bind PIWI-interacting RNAs (piRNAs) to silence transposons and maintain genome integrity in animal gonads. Here the authors present the crystal structure of a Drosophila Piwi–piRNA complex isolated from cultured fly ovarian somatic cells and find that it contains a non-canonica...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2020-02-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-020-14687-1 |
| _version_ | 1819042071505272832 |
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| author | Sonomi Yamaguchi Akira Oe Kazumichi M. Nishida Keitaro Yamashita Asako Kajiya Seiichi Hirano Naoki Matsumoto Naoshi Dohmae Ryuichiro Ishitani Kuniaki Saito Haruhiko Siomi Hiroshi Nishimasu Mikiko C. Siomi Osamu Nureki |
| author_facet | Sonomi Yamaguchi Akira Oe Kazumichi M. Nishida Keitaro Yamashita Asako Kajiya Seiichi Hirano Naoki Matsumoto Naoshi Dohmae Ryuichiro Ishitani Kuniaki Saito Haruhiko Siomi Hiroshi Nishimasu Mikiko C. Siomi Osamu Nureki |
| author_sort | Sonomi Yamaguchi |
| collection | DOAJ |
| description | PIWI-clade Argonautes bind PIWI-interacting RNAs (piRNAs) to silence transposons and maintain genome integrity in animal gonads. Here the authors present the crystal structure of a Drosophila Piwi–piRNA complex isolated from cultured fly ovarian somatic cells and find that it contains a non-canonical DVDK tetrad, and lacks slicer activity in vitro. |
| first_indexed | 2024-12-21T09:35:04Z |
| format | Article |
| id | doaj.art-9479ad5dbf9b4bffacfb69fcd7468085 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-21T09:35:04Z |
| publishDate | 2020-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-9479ad5dbf9b4bffacfb69fcd74680852022-12-21T19:08:39ZengNature PortfolioNature Communications2041-17232020-02-0111111310.1038/s41467-020-14687-1Crystal structure of Drosophila PiwiSonomi Yamaguchi0Akira Oe1Kazumichi M. Nishida2Keitaro Yamashita3Asako Kajiya4Seiichi Hirano5Naoki Matsumoto6Naoshi Dohmae7Ryuichiro Ishitani8Kuniaki Saito9Haruhiko Siomi10Hiroshi Nishimasu11Mikiko C. Siomi12Osamu Nureki13Department of Biological Sciences, Graduate School of Science, The University of TokyoDepartment of Biological Sciences, Graduate School of Science, The University of TokyoDepartment of Biological Sciences, Graduate School of Science, The University of TokyoDepartment of Biological Sciences, Graduate School of Science, The University of TokyoDepartment of Biological Sciences, Graduate School of Science, The University of TokyoDepartment of Biological Sciences, Graduate School of Science, The University of TokyoDepartment of Biological Sciences, Graduate School of Science, The University of TokyoBiomolecular Characterization Unit, RIKEN Center for Sustainable Resource ScienceDepartment of Biological Sciences, Graduate School of Science, The University of TokyoInvertebrate Genetics Laboratory, National Institute of GeneticsKeio University School of Medicine, 35 Shinanomachi, Shinjuku-kuDepartment of Biological Sciences, Graduate School of Science, The University of TokyoDepartment of Biological Sciences, Graduate School of Science, The University of TokyoDepartment of Biological Sciences, Graduate School of Science, The University of TokyoPIWI-clade Argonautes bind PIWI-interacting RNAs (piRNAs) to silence transposons and maintain genome integrity in animal gonads. Here the authors present the crystal structure of a Drosophila Piwi–piRNA complex isolated from cultured fly ovarian somatic cells and find that it contains a non-canonical DVDK tetrad, and lacks slicer activity in vitro.https://doi.org/10.1038/s41467-020-14687-1 |
| spellingShingle | Sonomi Yamaguchi Akira Oe Kazumichi M. Nishida Keitaro Yamashita Asako Kajiya Seiichi Hirano Naoki Matsumoto Naoshi Dohmae Ryuichiro Ishitani Kuniaki Saito Haruhiko Siomi Hiroshi Nishimasu Mikiko C. Siomi Osamu Nureki Crystal structure of Drosophila Piwi Nature Communications |
| title | Crystal structure of Drosophila Piwi |
| title_full | Crystal structure of Drosophila Piwi |
| title_fullStr | Crystal structure of Drosophila Piwi |
| title_full_unstemmed | Crystal structure of Drosophila Piwi |
| title_short | Crystal structure of Drosophila Piwi |
| title_sort | crystal structure of drosophila piwi |
| url | https://doi.org/10.1038/s41467-020-14687-1 |
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