Isolation and Purification of Protamine from the Cultured <i>Takifugu flavidus</i> and Its Physicochemical Properties
Protamine is a cationic peptide derived from fish sperm and has several important functional properties: antibacterial properties, acting as a carrier for injectable insulin and as a heparin antagonist, combatting fatigue, etc. Thus, it has been widely used in medicinal applications and food product...
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MDPI AG
2024-01-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/29/1/263 |
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| author | Shuji Liu Yue Zhang Yihui Chen Yongchang Su Bei Chen Yin Wang Min Xu Kun Qiao Shuigen Li Zhiyu Liu |
| author_facet | Shuji Liu Yue Zhang Yihui Chen Yongchang Su Bei Chen Yin Wang Min Xu Kun Qiao Shuigen Li Zhiyu Liu |
| author_sort | Shuji Liu |
| collection | DOAJ |
| description | Protamine is a cationic peptide derived from fish sperm and has several important functional properties: antibacterial properties, acting as a carrier for injectable insulin and as a heparin antagonist, combatting fatigue, etc. Thus, it has been widely used in medicinal applications and food products. Cultured <i>Takifugu flavidus</i> is a type of pufferfish with a delicious taste that is popular in China, and its production is increasing significantly. Therefore, protamine was extracted via acid extraction from the sperm of <i>Takifugu flavidus</i> and further isolated and purified via sephadex gel chromatography, ion exchange chromatography, and desalination chromatography. Furthermore, the physicochemical properties of protamine were investigated. The results showed that the sperm of the cultured <i>T. flavidus</i> were non-toxic, and the extracted and purified protamine had high contents of arginine (36.90%) and lysine (27.02%), respectively. The secondary structure of protamine was mainly β-folded and irregularly curled. Additionally, protamine exhibited high thermal stability with a denaturation temperature of 176 °C. This study would provide a theoretical basis for the structural analysis, bioactivity, and resource development of pufferfish protamine and help to promote the development of the pufferfish industry. |
| first_indexed | 2024-03-08T15:01:30Z |
| format | Article |
| id | doaj.art-947e11df187a40feb5fdac5a4c822d66 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-08T15:01:30Z |
| publishDate | 2024-01-01 |
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| series | Molecules |
| spelling | doaj.art-947e11df187a40feb5fdac5a4c822d662024-01-10T15:04:40ZengMDPI AGMolecules1420-30492024-01-0129126310.3390/molecules29010263Isolation and Purification of Protamine from the Cultured <i>Takifugu flavidus</i> and Its Physicochemical PropertiesShuji Liu0Yue Zhang1Yihui Chen2Yongchang Su3Bei Chen4Yin Wang5Min Xu6Kun Qiao7Shuigen Li8Zhiyu Liu9Key Laboratory of Cultivation and High-Value Utilization of Marine Organisms in Fujian Province, Fisheries Research Institute of Fujian, National Research and Development Center for Marine Fish Processing (Xiamen), Xiamen 361013, ChinaCollege of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaKey Laboratory of Cultivation and High-Value Utilization of Marine Organisms in Fujian Province, Fisheries Research Institute of Fujian, National Research and Development Center for Marine Fish Processing (Xiamen), Xiamen 361013, ChinaKey Laboratory of Cultivation and High-Value Utilization of Marine Organisms in Fujian Province, Fisheries Research Institute of Fujian, National Research and Development Center for Marine Fish Processing (Xiamen), Xiamen 361013, ChinaKey Laboratory of Cultivation and High-Value Utilization of Marine Organisms in Fujian Province, Fisheries Research Institute of Fujian, National Research and Development Center for Marine Fish Processing (Xiamen), Xiamen 361013, ChinaKey Laboratory of Cultivation and High-Value Utilization of Marine Organisms in Fujian Province, Fisheries Research Institute of Fujian, National Research and Development Center for Marine Fish Processing (Xiamen), Xiamen 361013, ChinaKey Laboratory of Cultivation and High-Value Utilization of Marine Organisms in Fujian Province, Fisheries Research Institute of Fujian, National Research and Development Center for Marine Fish Processing (Xiamen), Xiamen 361013, ChinaFujian Fisheries Technical Extension Station, Fuzhou 350002, ChinaKey Laboratory of Cultivation and High-Value Utilization of Marine Organisms in Fujian Province, Fisheries Research Institute of Fujian, National Research and Development Center for Marine Fish Processing (Xiamen), Xiamen 361013, ChinaProtamine is a cationic peptide derived from fish sperm and has several important functional properties: antibacterial properties, acting as a carrier for injectable insulin and as a heparin antagonist, combatting fatigue, etc. Thus, it has been widely used in medicinal applications and food products. Cultured <i>Takifugu flavidus</i> is a type of pufferfish with a delicious taste that is popular in China, and its production is increasing significantly. Therefore, protamine was extracted via acid extraction from the sperm of <i>Takifugu flavidus</i> and further isolated and purified via sephadex gel chromatography, ion exchange chromatography, and desalination chromatography. Furthermore, the physicochemical properties of protamine were investigated. The results showed that the sperm of the cultured <i>T. flavidus</i> were non-toxic, and the extracted and purified protamine had high contents of arginine (36.90%) and lysine (27.02%), respectively. The secondary structure of protamine was mainly β-folded and irregularly curled. Additionally, protamine exhibited high thermal stability with a denaturation temperature of 176 °C. This study would provide a theoretical basis for the structural analysis, bioactivity, and resource development of pufferfish protamine and help to promote the development of the pufferfish industry.https://www.mdpi.com/1420-3049/29/1/263cultured pufferfishprotaminepurificationproperties |
| spellingShingle | Shuji Liu Yue Zhang Yihui Chen Yongchang Su Bei Chen Yin Wang Min Xu Kun Qiao Shuigen Li Zhiyu Liu Isolation and Purification of Protamine from the Cultured <i>Takifugu flavidus</i> and Its Physicochemical Properties Molecules cultured pufferfish protamine purification properties |
| title | Isolation and Purification of Protamine from the Cultured <i>Takifugu flavidus</i> and Its Physicochemical Properties |
| title_full | Isolation and Purification of Protamine from the Cultured <i>Takifugu flavidus</i> and Its Physicochemical Properties |
| title_fullStr | Isolation and Purification of Protamine from the Cultured <i>Takifugu flavidus</i> and Its Physicochemical Properties |
| title_full_unstemmed | Isolation and Purification of Protamine from the Cultured <i>Takifugu flavidus</i> and Its Physicochemical Properties |
| title_short | Isolation and Purification of Protamine from the Cultured <i>Takifugu flavidus</i> and Its Physicochemical Properties |
| title_sort | isolation and purification of protamine from the cultured i takifugu flavidus i and its physicochemical properties |
| topic | cultured pufferfish protamine purification properties |
| url | https://www.mdpi.com/1420-3049/29/1/263 |
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