ASSESSMENT OF MOUSE EMBRYO VIABILITY BY ESTERASIC ACTIVITY DETECTION
In order to evaluate the esterasic activity within the viable embryos we used the Fluorescein diacetate (FDA) staining test. For staining was used a 0.5 mg/ml FDA stock solution. The embryos were recovered at 48 hours post coitus from superovulated Swiss mouse females. Before staining the embryos we...
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| Format: | Article |
| Language: | English |
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Agroprint Timisoara
2023-10-01
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| Series: | Scientific Papers Animal Science and Biotechnologies |
| Subjects: | |
| Online Access: | https://spasb.ro/index.php/public_html/article/view/1138 |
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| author | ALEXANDRA BOLEMAN ADA CEAN V. CARABĂ N. PĂCALĂ |
| author_facet | ALEXANDRA BOLEMAN ADA CEAN V. CARABĂ N. PĂCALĂ |
| author_sort | ALEXANDRA BOLEMAN |
| collection | DOAJ |
| description | In order to evaluate the esterasic activity within the viable embryos we used the
Fluorescein diacetate (FDA) staining test. For staining was used a 0.5 mg/ml FDA
stock solution. The embryos were recovered at 48 hours post coitus from
superovulated Swiss mouse females. Before staining the embryos were
microscopically evaluated by morphological criteria and classified in 4 quality
codes. The two methods used for quality and viability assessment were correlated
applying Pearson coefficient. The calculated value of the Pearson coefficient (r=1)
showed a strong correlation between the two methods used and indicate FDA
staining test and esterasic activity as a fast, easy and reliable method for embryo
viability assessment. |
| first_indexed | 2024-04-24T11:30:02Z |
| format | Article |
| id | doaj.art-9480b716a9d64a00b542650db5e3de2e |
| institution | Directory Open Access Journal |
| issn | 1841-9364 2344-4576 |
| language | English |
| last_indexed | 2024-04-24T11:30:02Z |
| publishDate | 2023-10-01 |
| publisher | Agroprint Timisoara |
| record_format | Article |
| series | Scientific Papers Animal Science and Biotechnologies |
| spelling | doaj.art-9480b716a9d64a00b542650db5e3de2e2024-04-10T10:38:35ZengAgroprint TimisoaraScientific Papers Animal Science and Biotechnologies1841-93642344-45762023-10-01421111138ASSESSMENT OF MOUSE EMBRYO VIABILITY BY ESTERASIC ACTIVITY DETECTIONALEXANDRA BOLEMAN0ADA CEAN1V. CARABĂ2N. PĂCALĂ3Faculty of Animal Sciences and Biotechnologies, TimisoaraFaculty of Animal Sciences and Biotechnologies, Timisoara Faculty of Animal Sciences and Biotechnologies, Timisoara Faculty of Animal Sciences and Biotechnologies, Timisoara In order to evaluate the esterasic activity within the viable embryos we used the Fluorescein diacetate (FDA) staining test. For staining was used a 0.5 mg/ml FDA stock solution. The embryos were recovered at 48 hours post coitus from superovulated Swiss mouse females. Before staining the embryos were microscopically evaluated by morphological criteria and classified in 4 quality codes. The two methods used for quality and viability assessment were correlated applying Pearson coefficient. The calculated value of the Pearson coefficient (r=1) showed a strong correlation between the two methods used and indicate FDA staining test and esterasic activity as a fast, easy and reliable method for embryo viability assessment.https://spasb.ro/index.php/public_html/article/view/1138embryoviabilityfdaesterasic activity |
| spellingShingle | ALEXANDRA BOLEMAN ADA CEAN V. CARABĂ N. PĂCALĂ ASSESSMENT OF MOUSE EMBRYO VIABILITY BY ESTERASIC ACTIVITY DETECTION Scientific Papers Animal Science and Biotechnologies embryo viability fda esterasic activity |
| title | ASSESSMENT OF MOUSE EMBRYO VIABILITY BY ESTERASIC ACTIVITY DETECTION |
| title_full | ASSESSMENT OF MOUSE EMBRYO VIABILITY BY ESTERASIC ACTIVITY DETECTION |
| title_fullStr | ASSESSMENT OF MOUSE EMBRYO VIABILITY BY ESTERASIC ACTIVITY DETECTION |
| title_full_unstemmed | ASSESSMENT OF MOUSE EMBRYO VIABILITY BY ESTERASIC ACTIVITY DETECTION |
| title_short | ASSESSMENT OF MOUSE EMBRYO VIABILITY BY ESTERASIC ACTIVITY DETECTION |
| title_sort | assessment of mouse embryo viability by esterasic activity detection |
| topic | embryo viability fda esterasic activity |
| url | https://spasb.ro/index.php/public_html/article/view/1138 |
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