We evaluated the use of a newly described sodC-based real-time-polymerase chain reaction (RT-PCR) assay for detecting Neisseria meningitidis in normally sterile sites, such as cerebrospinal fluid and serum. The sodC-based RT-PCR assay has an advantage over ctrA for detecting nongroupable N. meningit...

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Bibliographic Details
Main Authors: Fábio Takenori Higa, Lucila Okuyama Fukasawa, Maria Gisele Gonçalves, Maristela Marques Salgado, Ana Paula Silva de Lemos, Lee H Harrison, Priscilla Lima de Oliveira, Carla Naufal da Silva, Claudio Tavares Sacchi
Format: Article
Language:English
Published: Fundação Oswaldo Cruz (FIOCRUZ) 2013-04-01
Series:Memorias do Instituto Oswaldo Cruz
Subjects:
sodC-based RT-PCR
N. meningitidis diagnostics
meningitis
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000200246
Description
Summary:We evaluated the use of a newly described sodC-based real-time-polymerase chain reaction (RT-PCR) assay for detecting Neisseria meningitidis in normally sterile sites, such as cerebrospinal fluid and serum. The sodC-based RT-PCR assay has an advantage over ctrA for detecting nongroupable N. meningitidis isolates, which are commonly present in asymptomatic pharyngeal carriage. However, in our study, sodC-based RT-PCR was 7.5% less sensitive than ctrA. Given the public health impact of possible false-negative results due to the use of the sodC target gene alone, sodC-based RT-PCR for the diagnosis of meningococcal meningitis should be used with caution.
ISSN:0074-0276
1678-8060

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