We evaluated the use of a newly described sodC-based real-time-polymerase chain reaction (RT-PCR) assay for detecting Neisseria meningitidis in normally sterile sites, such as cerebrospinal fluid and serum. The sodC-based RT-PCR assay has an advantage over ctrA for detecting nongroupable N. meningit...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Fundação Oswaldo Cruz (FIOCRUZ)
2013-04-01
|
| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000200246 |
| _version_ | 1797758450088804352 |
|---|---|
| author | Fábio Takenori Higa Lucila Okuyama Fukasawa Maria Gisele Gonçalves Maristela Marques Salgado Ana Paula Silva de Lemos Lee H Harrison Priscilla Lima de Oliveira Carla Naufal da Silva Claudio Tavares Sacchi |
| author_facet | Fábio Takenori Higa Lucila Okuyama Fukasawa Maria Gisele Gonçalves Maristela Marques Salgado Ana Paula Silva de Lemos Lee H Harrison Priscilla Lima de Oliveira Carla Naufal da Silva Claudio Tavares Sacchi |
| author_sort | Fábio Takenori Higa |
| collection | DOAJ |
| description | We evaluated the use of a newly described sodC-based real-time-polymerase chain reaction (RT-PCR) assay for detecting Neisseria meningitidis in normally sterile sites, such as cerebrospinal fluid and serum. The sodC-based RT-PCR assay has an advantage over ctrA for detecting nongroupable N. meningitidis isolates, which are commonly present in asymptomatic pharyngeal carriage. However, in our study, sodC-based RT-PCR was 7.5% less sensitive than ctrA. Given the public health impact of possible false-negative results due to the use of the sodC target gene alone, sodC-based RT-PCR for the diagnosis of meningococcal meningitis should be used with caution. |
| first_indexed | 2024-03-12T18:30:08Z |
| format | Article |
| id | doaj.art-94828445606f4007935052ae1fac5675 |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T18:30:08Z |
| publishDate | 2013-04-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-94828445606f4007935052ae1fac56752023-08-02T08:19:30ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80602013-04-011082246247Fábio Takenori HigaLucila Okuyama FukasawaMaria Gisele GonçalvesMaristela Marques SalgadoAna Paula Silva de LemosLee H HarrisonPriscilla Lima de OliveiraCarla Naufal da SilvaClaudio Tavares SacchiWe evaluated the use of a newly described sodC-based real-time-polymerase chain reaction (RT-PCR) assay for detecting Neisseria meningitidis in normally sterile sites, such as cerebrospinal fluid and serum. The sodC-based RT-PCR assay has an advantage over ctrA for detecting nongroupable N. meningitidis isolates, which are commonly present in asymptomatic pharyngeal carriage. However, in our study, sodC-based RT-PCR was 7.5% less sensitive than ctrA. Given the public health impact of possible false-negative results due to the use of the sodC target gene alone, sodC-based RT-PCR for the diagnosis of meningococcal meningitis should be used with caution.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000200246sodC-based RT-PCRN. meningitidis diagnosticsmeningitis |
| spellingShingle | Fábio Takenori Higa Lucila Okuyama Fukasawa Maria Gisele Gonçalves Maristela Marques Salgado Ana Paula Silva de Lemos Lee H Harrison Priscilla Lima de Oliveira Carla Naufal da Silva Claudio Tavares Sacchi Memorias do Instituto Oswaldo Cruz sodC-based RT-PCR N. meningitidis diagnostics meningitis |
| topic | sodC-based RT-PCR N. meningitidis diagnostics meningitis |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762013000200246 |