Prophylactic Antibiofilm Activity of Antibiotic-Loaded Bone Cements against Gram-Negative Bacteria
Gram-negative bacilli can be responsible for prosthetic joint infection (PJI) even if staphylococci are the main involved pathogens. Gram-negative PJIs (GN-PJI) are considered difficult-to-treat infections due to the increase in antimicrobial resistance and biofilm formation. To minimize the risk of...
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MDPI AG
2022-01-01
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Online Access: | https://www.mdpi.com/2079-6382/11/2/137 |
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author | Andréa Cara Tristan Ferry Frédéric Laurent Jérôme Josse |
author_facet | Andréa Cara Tristan Ferry Frédéric Laurent Jérôme Josse |
author_sort | Andréa Cara |
collection | DOAJ |
description | Gram-negative bacilli can be responsible for prosthetic joint infection (PJI) even if staphylococci are the main involved pathogens. Gram-negative PJIs (GN-PJI) are considered difficult-to-treat infections due to the increase in antimicrobial resistance and biofilm formation. To minimize the risk of infection in cases of arthroplasties with cemented prosthesis, bone cement can be loaded with antibiotics, especially gentamicin. In this study, we aimed to compare the prophylactic antibiofilm activity of ready-to-use antibiotic-loaded bone cements (ALBC), already commercialized or new prototypes. We compared ALBCs containing gentamicin alone, gentamicin plus vancomycin, gentamicin plus clindamycin, gentamicin plus Fosfomycin, and fosfomycin alone, to plain cement (no antibiotic); these comparisons were conducted to investigate the biofilm formation of three strains of <i>Escherichia coli</i>, three strains of <i>Pseudomonas aeruginosa</i> and two strains of <i>Klebsiella pneumoniae</i>, with or without specific resistance to gentamicin or fosfomycin. We reported that ALBC containing gentamicin and clindamycin (COPAL G+C) seems to be the most interesting ALBC of our tested panel for the prevention of biofilm formation by gentamicin-susceptible strains, even if clindamycin is not effective against Gram-negative bacteria. However, gentamicin-resistant strains are still a problem, and further studies are needed to identify an antibiotic to associate with gentamicin for an efficient dual ALBC against Gram-negative bacteria. |
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language | English |
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spelling | doaj.art-94835590a1b947e4b24d409e675dcc412023-11-23T18:27:03ZengMDPI AGAntibiotics2079-63822022-01-0111213710.3390/antibiotics11020137Prophylactic Antibiofilm Activity of Antibiotic-Loaded Bone Cements against Gram-Negative BacteriaAndréa Cara0Tristan Ferry1Frédéric Laurent2Jérôme Josse3Centre International de Recherche en Infectiologie, CIRI, Inserm U1111, CNRS UMR5308, ENS de Lyon, UCBL1, 69007 Lyon, FranceCentre International de Recherche en Infectiologie, CIRI, Inserm U1111, CNRS UMR5308, ENS de Lyon, UCBL1, 69007 Lyon, FranceCentre International de Recherche en Infectiologie, CIRI, Inserm U1111, CNRS UMR5308, ENS de Lyon, UCBL1, 69007 Lyon, FranceCentre International de Recherche en Infectiologie, CIRI, Inserm U1111, CNRS UMR5308, ENS de Lyon, UCBL1, 69007 Lyon, FranceGram-negative bacilli can be responsible for prosthetic joint infection (PJI) even if staphylococci are the main involved pathogens. Gram-negative PJIs (GN-PJI) are considered difficult-to-treat infections due to the increase in antimicrobial resistance and biofilm formation. To minimize the risk of infection in cases of arthroplasties with cemented prosthesis, bone cement can be loaded with antibiotics, especially gentamicin. In this study, we aimed to compare the prophylactic antibiofilm activity of ready-to-use antibiotic-loaded bone cements (ALBC), already commercialized or new prototypes. We compared ALBCs containing gentamicin alone, gentamicin plus vancomycin, gentamicin plus clindamycin, gentamicin plus Fosfomycin, and fosfomycin alone, to plain cement (no antibiotic); these comparisons were conducted to investigate the biofilm formation of three strains of <i>Escherichia coli</i>, three strains of <i>Pseudomonas aeruginosa</i> and two strains of <i>Klebsiella pneumoniae</i>, with or without specific resistance to gentamicin or fosfomycin. We reported that ALBC containing gentamicin and clindamycin (COPAL G+C) seems to be the most interesting ALBC of our tested panel for the prevention of biofilm formation by gentamicin-susceptible strains, even if clindamycin is not effective against Gram-negative bacteria. However, gentamicin-resistant strains are still a problem, and further studies are needed to identify an antibiotic to associate with gentamicin for an efficient dual ALBC against Gram-negative bacteria.https://www.mdpi.com/2079-6382/11/2/137antibiotic-loaded bone cementprosthetic joint infectionbiofilm<i>Pseudomonas aeruginosa</i><i>Escherichia coli</i><i>Klebsiella pneumoniae</i> |
spellingShingle | Andréa Cara Tristan Ferry Frédéric Laurent Jérôme Josse Prophylactic Antibiofilm Activity of Antibiotic-Loaded Bone Cements against Gram-Negative Bacteria Antibiotics antibiotic-loaded bone cement prosthetic joint infection biofilm <i>Pseudomonas aeruginosa</i> <i>Escherichia coli</i> <i>Klebsiella pneumoniae</i> |
title | Prophylactic Antibiofilm Activity of Antibiotic-Loaded Bone Cements against Gram-Negative Bacteria |
title_full | Prophylactic Antibiofilm Activity of Antibiotic-Loaded Bone Cements against Gram-Negative Bacteria |
title_fullStr | Prophylactic Antibiofilm Activity of Antibiotic-Loaded Bone Cements against Gram-Negative Bacteria |
title_full_unstemmed | Prophylactic Antibiofilm Activity of Antibiotic-Loaded Bone Cements against Gram-Negative Bacteria |
title_short | Prophylactic Antibiofilm Activity of Antibiotic-Loaded Bone Cements against Gram-Negative Bacteria |
title_sort | prophylactic antibiofilm activity of antibiotic loaded bone cements against gram negative bacteria |
topic | antibiotic-loaded bone cement prosthetic joint infection biofilm <i>Pseudomonas aeruginosa</i> <i>Escherichia coli</i> <i>Klebsiella pneumoniae</i> |
url | https://www.mdpi.com/2079-6382/11/2/137 |
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