Genome-Wide Identification and Comprehensive Analysis of the <i>FtsH</i> Gene Family in Soybean (<i>Glycine max</i>)

Genome-Wide Identification and Comprehensive Analysis of the <i>FtsH</i> Gene Family in Soybean (<i>Glycine max</i>)

The filamentation temperature-sensitive H (<i>FtsH</i>) gene family is critical in regulating plant chloroplast development and photosynthesis. It plays a vital role in plant growth, development, and stress response. Although <i>FtsH</i> genes have been identified in a wide r...

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Bibliographic Details
Main Authors: Qi Shan, Baihui Zhou, Yuanxin Wang, Feiyu Hao, Lin Zhu, Yuhan Liu, Nan Wang, Fawei Wang, Xiaowei Li, Yuanyuan Dong, Keheng Xu, Yonggang Zhou, Haiyan Li, Weican Liu, Hongtao Gao
Format: Article
Language:English
Published: MDPI AG 2023-11-01
Series:International Journal of Molecular Sciences
Subjects:
soybean
<i>FtsH</i> family
chloroplast
protein hydrolysis enzyme
Online Access:https://www.mdpi.com/1422-0067/24/23/16996
Description
Summary:The filamentation temperature-sensitive H (<i>FtsH</i>) gene family is critical in regulating plant chloroplast development and photosynthesis. It plays a vital role in plant growth, development, and stress response. Although <i>FtsH</i> genes have been identified in a wide range of plants, there is no detailed study of the <i>FtsH</i> gene family in soybean (<i>Glycine max</i>). Here, we identified 34 <i>GmFtsH</i> genes, which could be categorized into eight groups, and <i>GmFtsH</i> genes in the same group had similar structures and conserved protein motifs. We also performed intraspecific and interspecific collinearity analysis and found that the <i>GmFtsH</i> family has large-scale gene duplication and is more closely related to <i>Arabidopsis thaliana</i>. Cis-acting elements analysis in the promoter region of the <i>GmFtsH</i> genes revealed that most genes contain developmental and stress response elements. Expression patterns based on transcriptome data and real-time reverse transcription quantitative PCR (qRT-PCR) showed that most of the <i>GmFtsH</i> genes were expressed at the highest levels in leaves. Then, GO enrichment analysis indicated that <i>GmFtsH</i> genes might function as a protein hydrolase. In addition, the GmFtsH13 protein was confirmed to be localized in chloroplasts by a transient expression experiment in tobacco. Taken together, the results of this study lay the foundation for the functional determination of <i>GmFtsH</i> genes and help researchers further understand the regulatory network in soybean leaf development.
ISSN:1661-6596
1422-0067

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