Decreased Expression of Estrogen Receptors Is Associated with Tumorigenesis in Papillary Thyroid Carcinoma

Papillary thyroid carcinomas (PTC), which is derived from thyroid follicular cells, is the most commonly differentiated thyroid cancer with sex disparity. However, the role of estrogen receptors (ERs) in the pathogenesis of PTC remains unclear. The present study aimed to determine the association of...

Full description

Bibliographic Details
Main Authors: Chen-Kai Chou, Shun-Yu Chi, Yi-Yung Hung, Yi-Chien Yang, Hung-Chun Fu, Jia-He Wang, Chueh-Chen Chen, Hong-Yo Kang
Format: Article
Language:English
Published: MDPI AG 2022-01-01
Series:International Journal of Molecular Sciences
Subjects:
Online Access:https://www.mdpi.com/1422-0067/23/3/1015
_version_ 1797487655514013696
author Chen-Kai Chou
Shun-Yu Chi
Yi-Yung Hung
Yi-Chien Yang
Hung-Chun Fu
Jia-He Wang
Chueh-Chen Chen
Hong-Yo Kang
author_facet Chen-Kai Chou
Shun-Yu Chi
Yi-Yung Hung
Yi-Chien Yang
Hung-Chun Fu
Jia-He Wang
Chueh-Chen Chen
Hong-Yo Kang
author_sort Chen-Kai Chou
collection DOAJ
description Papillary thyroid carcinomas (PTC), which is derived from thyroid follicular cells, is the most commonly differentiated thyroid cancer with sex disparity. However, the role of estrogen receptors (ERs) in the pathogenesis of PTC remains unclear. The present study aimed to determine the association of ER mRNA expression levels with clinicopathologic features in PTC. To that aim, the mRNA levels of <i>ESR1</i> (ERα66), <i>ESR1</i> (ERα36), <i>ESR2</i>, and G-protein-coupled estrogen receptor 1 (<i>GPER1</i>) in snap-frozen tissue samples from PTCs and adjacent normal thyroid tissues were determined using quantitative reverse transcription polymerase chain reaction (RT-qPCR), and the correlation between ER mRNA expression levels and clinicopathologic features was analyzed. The expression of ERα66, ERα36, ERβ, and <i>GPER1</i> was lower in PTC specimens than in adjacent normal thyroid tissues. Moreover, low <i>GPER1</i> expression was associated with extrathyroidal extension. There was no obvious difference in expression of ERs between PTC specimens from male and female patients. In conclusion, our findings highlight the importance of ERs in PTC tumorigenesis.
first_indexed 2024-03-09T23:51:46Z
format Article
id doaj.art-94876fab1fe9453e997aa88d201aac15
institution Directory Open Access Journal
issn 1661-6596
1422-0067
language English
last_indexed 2024-03-09T23:51:46Z
publishDate 2022-01-01
publisher MDPI AG
record_format Article
series International Journal of Molecular Sciences
spelling doaj.art-94876fab1fe9453e997aa88d201aac152023-11-23T16:33:38ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-01-01233101510.3390/ijms23031015Decreased Expression of Estrogen Receptors Is Associated with Tumorigenesis in Papillary Thyroid CarcinomaChen-Kai Chou0Shun-Yu Chi1Yi-Yung Hung2Yi-Chien Yang3Hung-Chun Fu4Jia-He Wang5Chueh-Chen Chen6Hong-Yo Kang7Division of Endocrinology and Metabolism, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, College of Medicine, Chang Gung University, Kaohsiung 83301, TaiwanDepartment of Surgery, Kaohsiung Chang Gung Memorial Hospital, College of Medicine, Chang Gung University, Kaohsiung 83301, TaiwanDepartment of Psychiatry, Kaohsiung Chang Gung Memorial Hospital, College of Medicine, Chang Gung University, Kaohsiung 83301, TaiwanDepartment of Dermatology, Kaohsiung Chang Gung Memorial Hospital, College of Medicine, Chang Gung University, Kaohsiung 83301, TaiwanDepartment of Obstetrics and Gynecology, Kaohsiung Chang Gung Memorial Hospital, College of Medicine, Chang Gung University, Kaohsiung 83301, TaiwanDivision of Endocrinology and Metabolism, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, College of Medicine, Chang Gung University, Kaohsiung 83301, TaiwanDivision of Endocrinology and Metabolism, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, College of Medicine, Chang Gung University, Kaohsiung 83301, TaiwanDepartment of Obstetrics and Gynecology, Kaohsiung Chang Gung Memorial Hospital, College of Medicine, Chang Gung University, Kaohsiung 83301, TaiwanPapillary thyroid carcinomas (PTC), which is derived from thyroid follicular cells, is the most commonly differentiated thyroid cancer with sex disparity. However, the role of estrogen receptors (ERs) in the pathogenesis of PTC remains unclear. The present study aimed to determine the association of ER mRNA expression levels with clinicopathologic features in PTC. To that aim, the mRNA levels of <i>ESR1</i> (ERα66), <i>ESR1</i> (ERα36), <i>ESR2</i>, and G-protein-coupled estrogen receptor 1 (<i>GPER1</i>) in snap-frozen tissue samples from PTCs and adjacent normal thyroid tissues were determined using quantitative reverse transcription polymerase chain reaction (RT-qPCR), and the correlation between ER mRNA expression levels and clinicopathologic features was analyzed. The expression of ERα66, ERα36, ERβ, and <i>GPER1</i> was lower in PTC specimens than in adjacent normal thyroid tissues. Moreover, low <i>GPER1</i> expression was associated with extrathyroidal extension. There was no obvious difference in expression of ERs between PTC specimens from male and female patients. In conclusion, our findings highlight the importance of ERs in PTC tumorigenesis.https://www.mdpi.com/1422-0067/23/3/1015papillary thyroid carcinomaestrogen receptorstumorigenesissex difference
spellingShingle Chen-Kai Chou
Shun-Yu Chi
Yi-Yung Hung
Yi-Chien Yang
Hung-Chun Fu
Jia-He Wang
Chueh-Chen Chen
Hong-Yo Kang
Decreased Expression of Estrogen Receptors Is Associated with Tumorigenesis in Papillary Thyroid Carcinoma
International Journal of Molecular Sciences
papillary thyroid carcinoma
estrogen receptors
tumorigenesis
sex difference
title Decreased Expression of Estrogen Receptors Is Associated with Tumorigenesis in Papillary Thyroid Carcinoma
title_full Decreased Expression of Estrogen Receptors Is Associated with Tumorigenesis in Papillary Thyroid Carcinoma
title_fullStr Decreased Expression of Estrogen Receptors Is Associated with Tumorigenesis in Papillary Thyroid Carcinoma
title_full_unstemmed Decreased Expression of Estrogen Receptors Is Associated with Tumorigenesis in Papillary Thyroid Carcinoma
title_short Decreased Expression of Estrogen Receptors Is Associated with Tumorigenesis in Papillary Thyroid Carcinoma
title_sort decreased expression of estrogen receptors is associated with tumorigenesis in papillary thyroid carcinoma
topic papillary thyroid carcinoma
estrogen receptors
tumorigenesis
sex difference
url https://www.mdpi.com/1422-0067/23/3/1015
work_keys_str_mv AT chenkaichou decreasedexpressionofestrogenreceptorsisassociatedwithtumorigenesisinpapillarythyroidcarcinoma
AT shunyuchi decreasedexpressionofestrogenreceptorsisassociatedwithtumorigenesisinpapillarythyroidcarcinoma
AT yiyunghung decreasedexpressionofestrogenreceptorsisassociatedwithtumorigenesisinpapillarythyroidcarcinoma
AT yichienyang decreasedexpressionofestrogenreceptorsisassociatedwithtumorigenesisinpapillarythyroidcarcinoma
AT hungchunfu decreasedexpressionofestrogenreceptorsisassociatedwithtumorigenesisinpapillarythyroidcarcinoma
AT jiahewang decreasedexpressionofestrogenreceptorsisassociatedwithtumorigenesisinpapillarythyroidcarcinoma
AT chuehchenchen decreasedexpressionofestrogenreceptorsisassociatedwithtumorigenesisinpapillarythyroidcarcinoma
AT hongyokang decreasedexpressionofestrogenreceptorsisassociatedwithtumorigenesisinpapillarythyroidcarcinoma