Decreased Expression of Estrogen Receptors Is Associated with Tumorigenesis in Papillary Thyroid Carcinoma
Papillary thyroid carcinomas (PTC), which is derived from thyroid follicular cells, is the most commonly differentiated thyroid cancer with sex disparity. However, the role of estrogen receptors (ERs) in the pathogenesis of PTC remains unclear. The present study aimed to determine the association of...
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MDPI AG
2022-01-01
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author | Chen-Kai Chou Shun-Yu Chi Yi-Yung Hung Yi-Chien Yang Hung-Chun Fu Jia-He Wang Chueh-Chen Chen Hong-Yo Kang |
author_facet | Chen-Kai Chou Shun-Yu Chi Yi-Yung Hung Yi-Chien Yang Hung-Chun Fu Jia-He Wang Chueh-Chen Chen Hong-Yo Kang |
author_sort | Chen-Kai Chou |
collection | DOAJ |
description | Papillary thyroid carcinomas (PTC), which is derived from thyroid follicular cells, is the most commonly differentiated thyroid cancer with sex disparity. However, the role of estrogen receptors (ERs) in the pathogenesis of PTC remains unclear. The present study aimed to determine the association of ER mRNA expression levels with clinicopathologic features in PTC. To that aim, the mRNA levels of <i>ESR1</i> (ERα66), <i>ESR1</i> (ERα36), <i>ESR2</i>, and G-protein-coupled estrogen receptor 1 (<i>GPER1</i>) in snap-frozen tissue samples from PTCs and adjacent normal thyroid tissues were determined using quantitative reverse transcription polymerase chain reaction (RT-qPCR), and the correlation between ER mRNA expression levels and clinicopathologic features was analyzed. The expression of ERα66, ERα36, ERβ, and <i>GPER1</i> was lower in PTC specimens than in adjacent normal thyroid tissues. Moreover, low <i>GPER1</i> expression was associated with extrathyroidal extension. There was no obvious difference in expression of ERs between PTC specimens from male and female patients. In conclusion, our findings highlight the importance of ERs in PTC tumorigenesis. |
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last_indexed | 2024-03-09T23:51:46Z |
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spelling | doaj.art-94876fab1fe9453e997aa88d201aac152023-11-23T16:33:38ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-01-01233101510.3390/ijms23031015Decreased Expression of Estrogen Receptors Is Associated with Tumorigenesis in Papillary Thyroid CarcinomaChen-Kai Chou0Shun-Yu Chi1Yi-Yung Hung2Yi-Chien Yang3Hung-Chun Fu4Jia-He Wang5Chueh-Chen Chen6Hong-Yo Kang7Division of Endocrinology and Metabolism, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, College of Medicine, Chang Gung University, Kaohsiung 83301, TaiwanDepartment of Surgery, Kaohsiung Chang Gung Memorial Hospital, College of Medicine, Chang Gung University, Kaohsiung 83301, TaiwanDepartment of Psychiatry, Kaohsiung Chang Gung Memorial Hospital, College of Medicine, Chang Gung University, Kaohsiung 83301, TaiwanDepartment of Dermatology, Kaohsiung Chang Gung Memorial Hospital, College of Medicine, Chang Gung University, Kaohsiung 83301, TaiwanDepartment of Obstetrics and Gynecology, Kaohsiung Chang Gung Memorial Hospital, College of Medicine, Chang Gung University, Kaohsiung 83301, TaiwanDivision of Endocrinology and Metabolism, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, College of Medicine, Chang Gung University, Kaohsiung 83301, TaiwanDivision of Endocrinology and Metabolism, Department of Internal Medicine, Kaohsiung Chang Gung Memorial Hospital, College of Medicine, Chang Gung University, Kaohsiung 83301, TaiwanDepartment of Obstetrics and Gynecology, Kaohsiung Chang Gung Memorial Hospital, College of Medicine, Chang Gung University, Kaohsiung 83301, TaiwanPapillary thyroid carcinomas (PTC), which is derived from thyroid follicular cells, is the most commonly differentiated thyroid cancer with sex disparity. However, the role of estrogen receptors (ERs) in the pathogenesis of PTC remains unclear. The present study aimed to determine the association of ER mRNA expression levels with clinicopathologic features in PTC. To that aim, the mRNA levels of <i>ESR1</i> (ERα66), <i>ESR1</i> (ERα36), <i>ESR2</i>, and G-protein-coupled estrogen receptor 1 (<i>GPER1</i>) in snap-frozen tissue samples from PTCs and adjacent normal thyroid tissues were determined using quantitative reverse transcription polymerase chain reaction (RT-qPCR), and the correlation between ER mRNA expression levels and clinicopathologic features was analyzed. The expression of ERα66, ERα36, ERβ, and <i>GPER1</i> was lower in PTC specimens than in adjacent normal thyroid tissues. Moreover, low <i>GPER1</i> expression was associated with extrathyroidal extension. There was no obvious difference in expression of ERs between PTC specimens from male and female patients. In conclusion, our findings highlight the importance of ERs in PTC tumorigenesis.https://www.mdpi.com/1422-0067/23/3/1015papillary thyroid carcinomaestrogen receptorstumorigenesissex difference |
spellingShingle | Chen-Kai Chou Shun-Yu Chi Yi-Yung Hung Yi-Chien Yang Hung-Chun Fu Jia-He Wang Chueh-Chen Chen Hong-Yo Kang Decreased Expression of Estrogen Receptors Is Associated with Tumorigenesis in Papillary Thyroid Carcinoma International Journal of Molecular Sciences papillary thyroid carcinoma estrogen receptors tumorigenesis sex difference |
title | Decreased Expression of Estrogen Receptors Is Associated with Tumorigenesis in Papillary Thyroid Carcinoma |
title_full | Decreased Expression of Estrogen Receptors Is Associated with Tumorigenesis in Papillary Thyroid Carcinoma |
title_fullStr | Decreased Expression of Estrogen Receptors Is Associated with Tumorigenesis in Papillary Thyroid Carcinoma |
title_full_unstemmed | Decreased Expression of Estrogen Receptors Is Associated with Tumorigenesis in Papillary Thyroid Carcinoma |
title_short | Decreased Expression of Estrogen Receptors Is Associated with Tumorigenesis in Papillary Thyroid Carcinoma |
title_sort | decreased expression of estrogen receptors is associated with tumorigenesis in papillary thyroid carcinoma |
topic | papillary thyroid carcinoma estrogen receptors tumorigenesis sex difference |
url | https://www.mdpi.com/1422-0067/23/3/1015 |
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