TAF15 promotes cell proliferation, migration and invasion of gastric cancer via activation of the RAF1/MEK/ERK signalling pathway
Abstract TATA-box-binding protein-associated Factor 15 (TAF15), a member of the FUS/EWS/TAF15 (FET) family, contributes to the progression of various tumours. However, the role and molecular mechanism of TAF15 in gastric cancer (GC) progression are still unknown. In this study, we found that TAF15 w...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-04-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-31959-0 |
| _version_ | 1797846024156348416 |
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| author | Li Tang Chengming Guo Xu Li Bo Zhang Liuye Huang |
| author_facet | Li Tang Chengming Guo Xu Li Bo Zhang Liuye Huang |
| author_sort | Li Tang |
| collection | DOAJ |
| description | Abstract TATA-box-binding protein-associated Factor 15 (TAF15), a member of the FUS/EWS/TAF15 (FET) family, contributes to the progression of various tumours. However, the role and molecular mechanism of TAF15 in gastric cancer (GC) progression are still unknown. In this study, we found that TAF15 was significantly upregulated in GC tumour tissues and cell lines. Overexpression of TAF15 was associated with a larger tumour size, high pathologic stage and high T stage of GC. TAF15 knockdown suppressed the proliferation, migration and invasion of GC cells in vitro and inhibited the tumour growth in vivo. Additionally, TAF15 knockdown led to the significant reductions in the phosphorylation levels of RAF1, MEK and ERK1/2, while total RAF1, MEK and ERK1/2 exhibited no significant change in GC cell lines. In summary, TAF15 is overexpressed in GC tumour tissues and cell lines, and promotes cell proliferation, migration and invasion in GC via the RAF1/MEK/ERK signaling pathway, which suggests that TAF15 might be a potential molecular diagnostic marker or therapeutic target for GC. |
| first_indexed | 2024-04-09T17:48:23Z |
| format | Article |
| id | doaj.art-94886044b7ad4e67aef4b69e8f327a54 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-09T17:48:23Z |
| publishDate | 2023-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-94886044b7ad4e67aef4b69e8f327a542023-04-16T11:11:02ZengNature PortfolioScientific Reports2045-23222023-04-0113111310.1038/s41598-023-31959-0TAF15 promotes cell proliferation, migration and invasion of gastric cancer via activation of the RAF1/MEK/ERK signalling pathwayLi Tang0Chengming Guo1Xu Li2Bo Zhang3Liuye Huang4Department of Gastroenterology, The Affiliated Yantai Yuhuangding Hospital of Qingdao UniversityDepartment of Gastroenterology, The Affiliated Yantai Yuhuangding Hospital of Qingdao UniversityDepartment of Gastroenterology, The Affiliated Yantai Yuhuangding Hospital of Qingdao UniversityDepartment of Gastroenterology, The Affiliated Yantai Yuhuangding Hospital of Qingdao UniversityDepartment of Gastroenterology, The Affiliated Yantai Yuhuangding Hospital of Qingdao UniversityAbstract TATA-box-binding protein-associated Factor 15 (TAF15), a member of the FUS/EWS/TAF15 (FET) family, contributes to the progression of various tumours. However, the role and molecular mechanism of TAF15 in gastric cancer (GC) progression are still unknown. In this study, we found that TAF15 was significantly upregulated in GC tumour tissues and cell lines. Overexpression of TAF15 was associated with a larger tumour size, high pathologic stage and high T stage of GC. TAF15 knockdown suppressed the proliferation, migration and invasion of GC cells in vitro and inhibited the tumour growth in vivo. Additionally, TAF15 knockdown led to the significant reductions in the phosphorylation levels of RAF1, MEK and ERK1/2, while total RAF1, MEK and ERK1/2 exhibited no significant change in GC cell lines. In summary, TAF15 is overexpressed in GC tumour tissues and cell lines, and promotes cell proliferation, migration and invasion in GC via the RAF1/MEK/ERK signaling pathway, which suggests that TAF15 might be a potential molecular diagnostic marker or therapeutic target for GC.https://doi.org/10.1038/s41598-023-31959-0 |
| spellingShingle | Li Tang Chengming Guo Xu Li Bo Zhang Liuye Huang TAF15 promotes cell proliferation, migration and invasion of gastric cancer via activation of the RAF1/MEK/ERK signalling pathway Scientific Reports |
| title | TAF15 promotes cell proliferation, migration and invasion of gastric cancer via activation of the RAF1/MEK/ERK signalling pathway |
| title_full | TAF15 promotes cell proliferation, migration and invasion of gastric cancer via activation of the RAF1/MEK/ERK signalling pathway |
| title_fullStr | TAF15 promotes cell proliferation, migration and invasion of gastric cancer via activation of the RAF1/MEK/ERK signalling pathway |
| title_full_unstemmed | TAF15 promotes cell proliferation, migration and invasion of gastric cancer via activation of the RAF1/MEK/ERK signalling pathway |
| title_short | TAF15 promotes cell proliferation, migration and invasion of gastric cancer via activation of the RAF1/MEK/ERK signalling pathway |
| title_sort | taf15 promotes cell proliferation migration and invasion of gastric cancer via activation of the raf1 mek erk signalling pathway |
| url | https://doi.org/10.1038/s41598-023-31959-0 |
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