Rapid Fabrication of Microfluidic Devices for Biological Mimicking: A Survey of Materials and Biocompatibility

Microfluidics is an essential technique used in the development of in vitro models for mimicking complex biological systems. The microchip with microfluidic flows offers the precise control of the microenvironment where the cells can grow and structure inside channels to resemble in vivo conditions...

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Main Authors: Hui Ling Ma, Ana Carolina Urbaczek, Fayene Zeferino Ribeiro de Souza, Paulo Augusto Gomes Garrido Carneiro Leão, Janice Rodrigues Perussi, Emanuel Carrilho
Format: Article
Language:English
Published: MDPI AG 2021-03-01
Series:Micromachines
Subjects:
Online Access:https://www.mdpi.com/2072-666X/12/3/346
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author Hui Ling Ma
Ana Carolina Urbaczek
Fayene Zeferino Ribeiro de Souza
Paulo Augusto Gomes Garrido Carneiro Leão
Janice Rodrigues Perussi
Emanuel Carrilho
author_facet Hui Ling Ma
Ana Carolina Urbaczek
Fayene Zeferino Ribeiro de Souza
Paulo Augusto Gomes Garrido Carneiro Leão
Janice Rodrigues Perussi
Emanuel Carrilho
author_sort Hui Ling Ma
collection DOAJ
description Microfluidics is an essential technique used in the development of in vitro models for mimicking complex biological systems. The microchip with microfluidic flows offers the precise control of the microenvironment where the cells can grow and structure inside channels to resemble in vivo conditions allowing a proper cellular response investigation. Hence, this study aimed to develop low-cost, simple microchips to simulate the shear stress effect on the human umbilical vein endothelial cells (HUVEC). Differentially from other biological microfluidic devices described in the literature, we used readily available tools like heat-lamination, toner printer, laser cutter and biocompatible double-sided adhesive tapes to bind different layers of materials together, forming a designed composite with a microchannel. In addition, we screened alternative substrates, including polyester-toner, polyester-vinyl, glass, Permanox<sup>®</sup> and polystyrene to compose the microchips for optimizing cell adhesion, then enabling these microdevices when coupled to a syringe pump, the cells can withstand the fluid shear stress range from 1 to 4 dyne cm<sup>2</sup>. The cell viability was monitored by acridine orange/ethidium bromide (AO/EB) staining to detect live and dead cells. As a result, our fabrication processes were cost-effective and straightforward. The materials investigated in the assembling of the microchips exhibited good cell viability and biocompatibility, providing a dynamic microenvironment for cell proliferation. Therefore, we suggest that these microchips could be available everywhere, allowing in vitro assays for daily laboratory experiments and further developing the organ-on-a-chip concept.
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spelling doaj.art-948927eff46b421ba3412fab91c711032023-11-21T11:45:06ZengMDPI AGMicromachines2072-666X2021-03-0112334610.3390/mi12030346Rapid Fabrication of Microfluidic Devices for Biological Mimicking: A Survey of Materials and BiocompatibilityHui Ling Ma0Ana Carolina Urbaczek1Fayene Zeferino Ribeiro de Souza2Paulo Augusto Gomes Garrido Carneiro Leão3Janice Rodrigues Perussi4Emanuel Carrilho5Instituto de Química de São Carlos, Universidade de São Paulo, São Carlos 13566-590, SP, BrazilInstituto de Química de São Carlos, Universidade de São Paulo, São Carlos 13566-590, SP, BrazilInstituto de Química de São Carlos, Universidade de São Paulo, São Carlos 13566-590, SP, BrazilInstituto de Química de São Carlos, Universidade de São Paulo, São Carlos 13566-590, SP, BrazilInstituto de Química de São Carlos, Universidade de São Paulo, São Carlos 13566-590, SP, BrazilInstituto de Química de São Carlos, Universidade de São Paulo, São Carlos 13566-590, SP, BrazilMicrofluidics is an essential technique used in the development of in vitro models for mimicking complex biological systems. The microchip with microfluidic flows offers the precise control of the microenvironment where the cells can grow and structure inside channels to resemble in vivo conditions allowing a proper cellular response investigation. Hence, this study aimed to develop low-cost, simple microchips to simulate the shear stress effect on the human umbilical vein endothelial cells (HUVEC). Differentially from other biological microfluidic devices described in the literature, we used readily available tools like heat-lamination, toner printer, laser cutter and biocompatible double-sided adhesive tapes to bind different layers of materials together, forming a designed composite with a microchannel. In addition, we screened alternative substrates, including polyester-toner, polyester-vinyl, glass, Permanox<sup>®</sup> and polystyrene to compose the microchips for optimizing cell adhesion, then enabling these microdevices when coupled to a syringe pump, the cells can withstand the fluid shear stress range from 1 to 4 dyne cm<sup>2</sup>. The cell viability was monitored by acridine orange/ethidium bromide (AO/EB) staining to detect live and dead cells. As a result, our fabrication processes were cost-effective and straightforward. The materials investigated in the assembling of the microchips exhibited good cell viability and biocompatibility, providing a dynamic microenvironment for cell proliferation. Therefore, we suggest that these microchips could be available everywhere, allowing in vitro assays for daily laboratory experiments and further developing the organ-on-a-chip concept.https://www.mdpi.com/2072-666X/12/3/346organ-on-chipshear stresspolyester-tonerpolyester-vinylpolystyreneglass
spellingShingle Hui Ling Ma
Ana Carolina Urbaczek
Fayene Zeferino Ribeiro de Souza
Paulo Augusto Gomes Garrido Carneiro Leão
Janice Rodrigues Perussi
Emanuel Carrilho
Rapid Fabrication of Microfluidic Devices for Biological Mimicking: A Survey of Materials and Biocompatibility
Micromachines
organ-on-chip
shear stress
polyester-toner
polyester-vinyl
polystyrene
glass
title Rapid Fabrication of Microfluidic Devices for Biological Mimicking: A Survey of Materials and Biocompatibility
title_full Rapid Fabrication of Microfluidic Devices for Biological Mimicking: A Survey of Materials and Biocompatibility
title_fullStr Rapid Fabrication of Microfluidic Devices for Biological Mimicking: A Survey of Materials and Biocompatibility
title_full_unstemmed Rapid Fabrication of Microfluidic Devices for Biological Mimicking: A Survey of Materials and Biocompatibility
title_short Rapid Fabrication of Microfluidic Devices for Biological Mimicking: A Survey of Materials and Biocompatibility
title_sort rapid fabrication of microfluidic devices for biological mimicking a survey of materials and biocompatibility
topic organ-on-chip
shear stress
polyester-toner
polyester-vinyl
polystyrene
glass
url https://www.mdpi.com/2072-666X/12/3/346
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