Electrochemical Characteristics of <i>Shewanella loihica</i> PV-4 on Reticulated Vitreous Carbon (RVC) with Different Potentials Applied
The current output of an anodic bioelectrochemical system (BES) depends upon the extracellular electron transfer (EET) rate from electricigens to the electrodes. Thus, investigation of EET mechanisms between electricigens and solid electrodes is essential. Here, reticulated vitreous carbon (RVC) ele...
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MDPI AG
2022-08-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/16/5330 |
| _version_ | 1797431839847088128 |
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| author | Shixin Wang Xiaoming Zhang Enrico Marsili |
| author_facet | Shixin Wang Xiaoming Zhang Enrico Marsili |
| author_sort | Shixin Wang |
| collection | DOAJ |
| description | The current output of an anodic bioelectrochemical system (BES) depends upon the extracellular electron transfer (EET) rate from electricigens to the electrodes. Thus, investigation of EET mechanisms between electricigens and solid electrodes is essential. Here, reticulated vitreous carbon (RVC) electrodes are used to increase the surface available for biofilm formation of the known electricigen <i>Shewanella loihica</i> PV-4, which is limited in conventional flat electrodes. <i>S. loihica</i> PV-4 utilizes flavin-mediated EET at potential lower than the outer membrane cytochromes (OMC), while at higher potential, both direct electron transfer (DET) and mediated electron transfer (MET) contribute to the current output. Results show that high electrode potential favors cell attachment on RVC, which enhances the current output. DET is the prevailing mechanism in early biofilm, while the contribution of MET to current output increased as the biofilm matured. Electrochemical analysis under starvation shows that the mediators could be confined in the biofilm. The morphology of biofilm shows bacteria distributed on the top layer of honeycomb structures, preferentially on the flat areas. This study provides insights into the EET pathways of <i>S. loihica</i> PV-4 on porous RVC electrodes at different biofilm ages and different set potential, which is important for the design of real-world BES. |
| first_indexed | 2024-03-09T09:51:05Z |
| format | Article |
| id | doaj.art-948e39bf2295425db61273442ae60d34 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T09:51:05Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-948e39bf2295425db61273442ae60d342023-12-02T00:05:28ZengMDPI AGMolecules1420-30492022-08-012716533010.3390/molecules27165330Electrochemical Characteristics of <i>Shewanella loihica</i> PV-4 on Reticulated Vitreous Carbon (RVC) with Different Potentials AppliedShixin Wang0Xiaoming Zhang1Enrico Marsili2School of Science, Minzu University of China, Beijing 100081, ChinaSchool of Science, Minzu University of China, Beijing 100081, ChinaDepartment of Chemical and Materials Engineering, School of Engineering and Digital Sciences, Nazarbayev University, Nur-Sultan 010000, KazakhstanThe current output of an anodic bioelectrochemical system (BES) depends upon the extracellular electron transfer (EET) rate from electricigens to the electrodes. Thus, investigation of EET mechanisms between electricigens and solid electrodes is essential. Here, reticulated vitreous carbon (RVC) electrodes are used to increase the surface available for biofilm formation of the known electricigen <i>Shewanella loihica</i> PV-4, which is limited in conventional flat electrodes. <i>S. loihica</i> PV-4 utilizes flavin-mediated EET at potential lower than the outer membrane cytochromes (OMC), while at higher potential, both direct electron transfer (DET) and mediated electron transfer (MET) contribute to the current output. Results show that high electrode potential favors cell attachment on RVC, which enhances the current output. DET is the prevailing mechanism in early biofilm, while the contribution of MET to current output increased as the biofilm matured. Electrochemical analysis under starvation shows that the mediators could be confined in the biofilm. The morphology of biofilm shows bacteria distributed on the top layer of honeycomb structures, preferentially on the flat areas. This study provides insights into the EET pathways of <i>S. loihica</i> PV-4 on porous RVC electrodes at different biofilm ages and different set potential, which is important for the design of real-world BES.https://www.mdpi.com/1420-3049/27/16/5330reticulated vitreous carbon (RVC)<i>Shewanella loihica</i> PV-4biofilm formationdirect electron transfer (DET)mediated electron transfer (MET)bioelectrochemical systems (BES) |
| spellingShingle | Shixin Wang Xiaoming Zhang Enrico Marsili Electrochemical Characteristics of <i>Shewanella loihica</i> PV-4 on Reticulated Vitreous Carbon (RVC) with Different Potentials Applied Molecules reticulated vitreous carbon (RVC) <i>Shewanella loihica</i> PV-4 biofilm formation direct electron transfer (DET) mediated electron transfer (MET) bioelectrochemical systems (BES) |
| title | Electrochemical Characteristics of <i>Shewanella loihica</i> PV-4 on Reticulated Vitreous Carbon (RVC) with Different Potentials Applied |
| title_full | Electrochemical Characteristics of <i>Shewanella loihica</i> PV-4 on Reticulated Vitreous Carbon (RVC) with Different Potentials Applied |
| title_fullStr | Electrochemical Characteristics of <i>Shewanella loihica</i> PV-4 on Reticulated Vitreous Carbon (RVC) with Different Potentials Applied |
| title_full_unstemmed | Electrochemical Characteristics of <i>Shewanella loihica</i> PV-4 on Reticulated Vitreous Carbon (RVC) with Different Potentials Applied |
| title_short | Electrochemical Characteristics of <i>Shewanella loihica</i> PV-4 on Reticulated Vitreous Carbon (RVC) with Different Potentials Applied |
| title_sort | electrochemical characteristics of i shewanella loihica i pv 4 on reticulated vitreous carbon rvc with different potentials applied |
| topic | reticulated vitreous carbon (RVC) <i>Shewanella loihica</i> PV-4 biofilm formation direct electron transfer (DET) mediated electron transfer (MET) bioelectrochemical systems (BES) |
| url | https://www.mdpi.com/1420-3049/27/16/5330 |
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