Connective tissue mast cells store and release noradrenaline
Abstract Mast cells are present in mucosal and connective tissues throughout the body. They synthesize and release a wide variety of bioactive molecules, such as histamine, proteases, and cytokines. In this study, we found that a population of connective tissue mast cells (CTMCs) stores and releases...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-10-01
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| Series: | Journal of Physiological Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12576-023-00883-3 |
| _version_ | 1797453620616101888 |
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| author | Yusuke Otani Soichiro Yoshikawa Kei Nagao Takehiro Tanaka Shinichi Toyooka Atsushi Fujimura |
| author_facet | Yusuke Otani Soichiro Yoshikawa Kei Nagao Takehiro Tanaka Shinichi Toyooka Atsushi Fujimura |
| author_sort | Yusuke Otani |
| collection | DOAJ |
| description | Abstract Mast cells are present in mucosal and connective tissues throughout the body. They synthesize and release a wide variety of bioactive molecules, such as histamine, proteases, and cytokines. In this study, we found that a population of connective tissue mast cells (CTMCs) stores and releases noradrenaline, originating from sympathetic nerves. Noradrenaline-storing cells, not neuronal fibers, were predominantly identified in the connective tissues of the skin, mammary gland, gastrointestinal tract, bronchus, thymus, and pancreas in wild-type mice but were absent in mast cell–deficient W-sash c-kit mutant Kit W−sh/W−sh mice. In vitro studies using bone marrow–derived mast cells revealed that extracellular noradrenaline was taken up but not synthesized. Upon ionomycin stimulation, noradrenaline was released. Electron microscopy analyses further suggested that noradrenaline is stored in and released from the secretory granules of mast cells. Finally, we found that noradrenaline-storing CTMCs express organic cation transporter 3 (Oct3), which is also known as an extraneuronal monoamine transporter, SLC22A3. Our findings indicate that mast cells may play a role in regulating noradrenaline concentration by storing and releasing it in somatic tissues. |
| first_indexed | 2024-03-09T15:25:27Z |
| format | Article |
| id | doaj.art-9497b0ef078f4d3aa4a2210098957a8a |
| institution | Directory Open Access Journal |
| issn | 1880-6562 |
| language | English |
| last_indexed | 2024-03-09T15:25:27Z |
| publishDate | 2023-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Physiological Sciences |
| spelling | doaj.art-9497b0ef078f4d3aa4a2210098957a8a2023-11-26T12:34:15ZengBMCJournal of Physiological Sciences1880-65622023-10-0173111410.1186/s12576-023-00883-3Connective tissue mast cells store and release noradrenalineYusuke Otani0Soichiro Yoshikawa1Kei Nagao2Takehiro Tanaka3Shinichi Toyooka4Atsushi Fujimura5Department of General Thoracic Surgery and Breast and Endocrinological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesDepartment of Cellular Physiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesDepartment of Cellular Physiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesDepartment of Pathology and Oncology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesDepartment of General Thoracic Surgery and Breast and Endocrinological Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesDepartment of Cellular Physiology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical SciencesAbstract Mast cells are present in mucosal and connective tissues throughout the body. They synthesize and release a wide variety of bioactive molecules, such as histamine, proteases, and cytokines. In this study, we found that a population of connective tissue mast cells (CTMCs) stores and releases noradrenaline, originating from sympathetic nerves. Noradrenaline-storing cells, not neuronal fibers, were predominantly identified in the connective tissues of the skin, mammary gland, gastrointestinal tract, bronchus, thymus, and pancreas in wild-type mice but were absent in mast cell–deficient W-sash c-kit mutant Kit W−sh/W−sh mice. In vitro studies using bone marrow–derived mast cells revealed that extracellular noradrenaline was taken up but not synthesized. Upon ionomycin stimulation, noradrenaline was released. Electron microscopy analyses further suggested that noradrenaline is stored in and released from the secretory granules of mast cells. Finally, we found that noradrenaline-storing CTMCs express organic cation transporter 3 (Oct3), which is also known as an extraneuronal monoamine transporter, SLC22A3. Our findings indicate that mast cells may play a role in regulating noradrenaline concentration by storing and releasing it in somatic tissues.https://doi.org/10.1186/s12576-023-00883-3Mast cellsConnective tissue mast cellsNoradrenalineImmunoelectron microscopySLC22A3 |
| spellingShingle | Yusuke Otani Soichiro Yoshikawa Kei Nagao Takehiro Tanaka Shinichi Toyooka Atsushi Fujimura Connective tissue mast cells store and release noradrenaline Journal of Physiological Sciences Mast cells Connective tissue mast cells Noradrenaline Immunoelectron microscopy SLC22A3 |
| title | Connective tissue mast cells store and release noradrenaline |
| title_full | Connective tissue mast cells store and release noradrenaline |
| title_fullStr | Connective tissue mast cells store and release noradrenaline |
| title_full_unstemmed | Connective tissue mast cells store and release noradrenaline |
| title_short | Connective tissue mast cells store and release noradrenaline |
| title_sort | connective tissue mast cells store and release noradrenaline |
| topic | Mast cells Connective tissue mast cells Noradrenaline Immunoelectron microscopy SLC22A3 |
| url | https://doi.org/10.1186/s12576-023-00883-3 |
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