Development of Insulin Resistance through Induction of miRNA-135 in C2C12 Cells
Objective: Micro-RNAs (miRNAs) are a class of posttranscriptional regulators that play crucial roles in various biological processes. Emerging evidence suggests a direct link between miRNAs and development of several diseases including type 2 diabetes (T2D). In this study, we aimed to investigate...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Royan Institute (ACECR), Tehran
2016-09-01
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| Series: | Cell Journal |
| Subjects: | |
| Online Access: | http://celljournal.org/web/journal/article/6104/download |
| _version_ | 1818035921228922880 |
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| author | Maryam Honardoost Ehsan Arefian Masoud Soleimani Sara Soudi Mohammad Reza Sarookhani |
| author_facet | Maryam Honardoost Ehsan Arefian Masoud Soleimani Sara Soudi Mohammad Reza Sarookhani |
| author_sort | Maryam Honardoost |
| collection | DOAJ |
| description | Objective: Micro-RNAs (miRNAs) are a class of posttranscriptional regulators that play
crucial roles in various biological processes. Emerging evidence suggests a direct link
between miRNAs and development of several diseases including type 2 diabetes (T2D).
In this study, we aimed to investigate the effect of predicted miRNA and target genes on
insulin resistance.
Materials and Methods: This experimental study was conducted on the C2C12 cell line.
Using bioinformatics tools miRNA-135 and two respective target genes-insulin receptor
(Insr) and vesicle associated membrane protein 2 (Vamp2)- were selected as potential
factors involved in insulin resistance process. Levels of glucose uptake miRNA expression
and respective gene targets were determined after cell transfaction by miR-135.
Results: It was determined that Insr gene expression was significantly down-regulated
in miR-135 transfected C2C12 cell line (P≤0.05). Interestingly; these transfected cells
have shown a significant difference in glucose uptake incomparision the positive control
cells, while it was similar to the insulin resistant cell line (P≤0.05). In contrast, no significant
alteration of Vamp2 gene expression was observed.
Conclusion: Our data indicated no change on the Vamp2 expression level after miRNA
transfection, while expression level of Insr was reduced and miR-135 expression
was contrarily increased leading to poor stimulation of glucose uptake through insulin,
and development of insulin resistance phenotype in C2C12 cell line. |
| first_indexed | 2024-12-10T07:02:44Z |
| format | Article |
| id | doaj.art-9497be9b4ea240529fd222d68b4830f3 |
| institution | Directory Open Access Journal |
| issn | 2228-5806 2228-5814 |
| language | English |
| last_indexed | 2024-12-10T07:02:44Z |
| publishDate | 2016-09-01 |
| publisher | Royan Institute (ACECR), Tehran |
| record_format | Article |
| series | Cell Journal |
| spelling | doaj.art-9497be9b4ea240529fd222d68b4830f32022-12-22T01:58:17ZengRoyan Institute (ACECR), TehranCell Journal2228-58062228-58142016-09-01183353361Development of Insulin Resistance through Induction of miRNA-135 in C2C12 CellsMaryam Honardoost0Ehsan Arefian1Masoud Soleimani2Sara Soudi3Mohammad Reza Sarookhani4Department of Molecular Medicine, School of Medicine, Qazvin University of Medical Sciences, Qazvin, IranDepartment of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, IranDepartment of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IranDepartment of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, IranDepartment of Molecular Medicine, School of Medicine, Qazvin University of Medical Sciences, Qazvin, IranObjective: Micro-RNAs (miRNAs) are a class of posttranscriptional regulators that play crucial roles in various biological processes. Emerging evidence suggests a direct link between miRNAs and development of several diseases including type 2 diabetes (T2D). In this study, we aimed to investigate the effect of predicted miRNA and target genes on insulin resistance. Materials and Methods: This experimental study was conducted on the C2C12 cell line. Using bioinformatics tools miRNA-135 and two respective target genes-insulin receptor (Insr) and vesicle associated membrane protein 2 (Vamp2)- were selected as potential factors involved in insulin resistance process. Levels of glucose uptake miRNA expression and respective gene targets were determined after cell transfaction by miR-135. Results: It was determined that Insr gene expression was significantly down-regulated in miR-135 transfected C2C12 cell line (P≤0.05). Interestingly; these transfected cells have shown a significant difference in glucose uptake incomparision the positive control cells, while it was similar to the insulin resistant cell line (P≤0.05). In contrast, no significant alteration of Vamp2 gene expression was observed. Conclusion: Our data indicated no change on the Vamp2 expression level after miRNA transfection, while expression level of Insr was reduced and miR-135 expression was contrarily increased leading to poor stimulation of glucose uptake through insulin, and development of insulin resistance phenotype in C2C12 cell line.http://celljournal.org/web/journal/article/6104/downloadInsulin ResistanceMiR-135Insulin ReceptorC2C12 |
| spellingShingle | Maryam Honardoost Ehsan Arefian Masoud Soleimani Sara Soudi Mohammad Reza Sarookhani Development of Insulin Resistance through Induction of miRNA-135 in C2C12 Cells Cell Journal Insulin Resistance MiR-135 Insulin Receptor C2C12 |
| title | Development of Insulin Resistance through Induction of miRNA-135 in C2C12 Cells |
| title_full | Development of Insulin Resistance through Induction of miRNA-135 in C2C12 Cells |
| title_fullStr | Development of Insulin Resistance through Induction of miRNA-135 in C2C12 Cells |
| title_full_unstemmed | Development of Insulin Resistance through Induction of miRNA-135 in C2C12 Cells |
| title_short | Development of Insulin Resistance through Induction of miRNA-135 in C2C12 Cells |
| title_sort | development of insulin resistance through induction of mirna 135 in c2c12 cells |
| topic | Insulin Resistance MiR-135 Insulin Receptor C2C12 |
| url | http://celljournal.org/web/journal/article/6104/download |
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