Evaluation of Carboxymethyl-Hexanoyl Chitosan as a Protein Nanocarrier
Carboxymethyl‐hexanoyl chitosan (CHC) has the ability to self‐assemble into nanocapsules in an aqueous solution and it has recently shown potential in numerous biomedical applications. Here we investigate the protein loading efficiency and release, as well as the structural properties of CHC protein...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Hindawi - SAGE Publishing
2013-06-01
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| Series: | Nanomaterials and Nanotechnology |
| Subjects: | |
| Online Access: | http://www.intechopen.com/journals/nanomaterials_and_nanotechnology/evaluation-of-carboxymethyl-hexanoyl-chitosan-as-a-protein-nanocarrier |
| _version_ | 1797642452619755520 |
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| author | Mikael Larsson Annika Borde Emma Mattisson Dean-Mo Liu Anette Larsson |
| author_facet | Mikael Larsson Annika Borde Emma Mattisson Dean-Mo Liu Anette Larsson |
| author_sort | Mikael Larsson |
| collection | DOAJ |
| description | Carboxymethyl‐hexanoyl chitosan (CHC) has
the ability to self‐assemble into nanocapsules in an
aqueous solution and it has recently shown potential
in numerous biomedical applications. Here we
investigate the protein loading efficiency and release,
as well as the structural properties of CHC protein
nanocarriers. Bovine serum albumin (BSA) or its
chromophore labelled version, fluorescein‐BSA, was
used as a model protein and the loading was
performed with a simple mixing of pre‐formed
nanocapsules and protein. Dynamic light scattering
and zeta potential analysis revealed that protein
loaded nanocarriers with high positive zeta potential
were formed. The protein loaded nanocarriers
displayed a loading efficiency of 75% and a very slow
protein release. In summary, our results highlight the
potential of CHC as a protein nanocarrier, but also
indicate that protein‐CHC interactions need to be
considered in protein containing CHC formulations
where protein release is not the main function. |
| first_indexed | 2024-03-11T14:00:25Z |
| format | Article |
| id | doaj.art-949922293a1145b4ac11237978ead541 |
| institution | Directory Open Access Journal |
| issn | 1847-9804 |
| language | English |
| last_indexed | 2024-03-11T14:00:25Z |
| publishDate | 2013-06-01 |
| publisher | Hindawi - SAGE Publishing |
| record_format | Article |
| series | Nanomaterials and Nanotechnology |
| spelling | doaj.art-949922293a1145b4ac11237978ead5412023-11-02T04:55:23ZengHindawi - SAGE PublishingNanomaterials and Nanotechnology1847-98042013-06-0137http://dx.doi.org/10.5772/5676845356Evaluation of Carboxymethyl-Hexanoyl Chitosan as a Protein NanocarrierMikael LarssonAnnika BordeEmma MattissonDean-Mo LiuAnette LarssonCarboxymethyl‐hexanoyl chitosan (CHC) has the ability to self‐assemble into nanocapsules in an aqueous solution and it has recently shown potential in numerous biomedical applications. Here we investigate the protein loading efficiency and release, as well as the structural properties of CHC protein nanocarriers. Bovine serum albumin (BSA) or its chromophore labelled version, fluorescein‐BSA, was used as a model protein and the loading was performed with a simple mixing of pre‐formed nanocapsules and protein. Dynamic light scattering and zeta potential analysis revealed that protein loaded nanocarriers with high positive zeta potential were formed. The protein loaded nanocarriers displayed a loading efficiency of 75% and a very slow protein release. In summary, our results highlight the potential of CHC as a protein nanocarrier, but also indicate that protein‐CHC interactions need to be considered in protein containing CHC formulations where protein release is not the main function.http://www.intechopen.com/journals/nanomaterials_and_nanotechnology/evaluation-of-carboxymethyl-hexanoyl-chitosan-as-a-protein-nanocarrierCarboxymethyl‐Hexanoyl ChitosanAmphiphilic ChitosanNanocarrierProtein DeliveryNanoparticle |
| spellingShingle | Mikael Larsson Annika Borde Emma Mattisson Dean-Mo Liu Anette Larsson Evaluation of Carboxymethyl-Hexanoyl Chitosan as a Protein Nanocarrier Nanomaterials and Nanotechnology Carboxymethyl‐Hexanoyl Chitosan Amphiphilic Chitosan Nanocarrier Protein Delivery Nanoparticle |
| title | Evaluation of Carboxymethyl-Hexanoyl Chitosan as a Protein Nanocarrier |
| title_full | Evaluation of Carboxymethyl-Hexanoyl Chitosan as a Protein Nanocarrier |
| title_fullStr | Evaluation of Carboxymethyl-Hexanoyl Chitosan as a Protein Nanocarrier |
| title_full_unstemmed | Evaluation of Carboxymethyl-Hexanoyl Chitosan as a Protein Nanocarrier |
| title_short | Evaluation of Carboxymethyl-Hexanoyl Chitosan as a Protein Nanocarrier |
| title_sort | evaluation of carboxymethyl hexanoyl chitosan as a protein nanocarrier |
| topic | Carboxymethyl‐Hexanoyl Chitosan Amphiphilic Chitosan Nanocarrier Protein Delivery Nanoparticle |
| url | http://www.intechopen.com/journals/nanomaterials_and_nanotechnology/evaluation-of-carboxymethyl-hexanoyl-chitosan-as-a-protein-nanocarrier |
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