Comparison of phenotypic characterization between differentiated osteoblasts from stem cells and Calvaria osteoblasts In vitro
Background: Characteristics of differentiated osteoblasts from adipose derived stem cells (ADSCs) in compared with isolated osteoblasts from normal bone such as calvaria are unknown. The aim of this study was determination and comparison of phenotypic characterization between differentiated osteobla...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2013-01-01
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| Series: | International Journal of Preventive Medicine |
| Subjects: | |
| Online Access: | http://www.ijpvmjournal.net/article.asp?issn=2008-7802;year=2013;volume=4;issue=2;spage=180;epage=186;aulast=Hashemibeni |
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| author | Batool Hashemibeni Farzaneh Jafary Nafiseh Esmaeil Vahid Goharian Ghader Feizi Fariba Heidari Sayyedeh Zahra Moosavi-Nejad Sayyed Hamid Zarkesh |
| author_facet | Batool Hashemibeni Farzaneh Jafary Nafiseh Esmaeil Vahid Goharian Ghader Feizi Fariba Heidari Sayyedeh Zahra Moosavi-Nejad Sayyed Hamid Zarkesh |
| author_sort | Batool Hashemibeni |
| collection | DOAJ |
| description | Background: Characteristics of differentiated osteoblasts from adipose derived stem cells (ADSCs) in compared with isolated osteoblasts from normal bone such as calvaria are unknown. The aim of this study was determination and comparison of phenotypic characterization between differentiated osteoblasts from stem cells and calvaria osteoblasts in vitro.
Methods: In this study, mesenchymal stem cells were isolated from adipose tissue of human by enzymatic digestion and were differentiated into osteoblasts using osteogenic medium. Characteristics of these cells at first, second, third and fourth weeks were comprised with calvaria osteoblasts that were isolated from human calvaria by explanation culture method. To screen the characteristics of both calvaria and the differentiated osteoblasts, we used western blot to identify protein levels, von Kossa staining for mineral matrix detection and alkaline phosphatase (ALP) assay kit (Sigma) for ALP activity measurement. Difference between calvaria and differentiated osteoblast cells were analyzed by one-way ANOVA and P0 < 0.05 was considered as statistically significant.
Results: Alkaline phosphatase activity, collagen and mineral material production in differentiated osteoblasts at third week were more significantly than calvaria cells ( P < 0.05). Our results indicated that there was no significant different in osteocalcin (OC) production between differentiated osteoblast at first, second and third weeks and calvaria cells but declined at fourth week (P < 0.05).
Conclusions: Our survey showed that cellular traits of differentiated osteoblasts presented better than calvaria osteoblasts in vitro conditions. Therefore, we suggest that ADSCs could be used in next studies for bone tissue engineering. |
| first_indexed | 2024-12-12T04:00:20Z |
| format | Article |
| id | doaj.art-9499a9a60f22451ca434c110022404ae |
| institution | Directory Open Access Journal |
| issn | 2008-7802 2008-8213 |
| language | English |
| last_indexed | 2024-12-12T04:00:20Z |
| publishDate | 2013-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | International Journal of Preventive Medicine |
| spelling | doaj.art-9499a9a60f22451ca434c110022404ae2022-12-22T00:39:05ZengWolters Kluwer Medknow PublicationsInternational Journal of Preventive Medicine2008-78022008-82132013-01-0142180186Comparison of phenotypic characterization between differentiated osteoblasts from stem cells and Calvaria osteoblasts In vitroBatool HashemibeniFarzaneh JafaryNafiseh EsmaeilVahid GoharianGhader FeiziFariba HeidariSayyedeh Zahra Moosavi-NejadSayyed Hamid ZarkeshBackground: Characteristics of differentiated osteoblasts from adipose derived stem cells (ADSCs) in compared with isolated osteoblasts from normal bone such as calvaria are unknown. The aim of this study was determination and comparison of phenotypic characterization between differentiated osteoblasts from stem cells and calvaria osteoblasts in vitro. Methods: In this study, mesenchymal stem cells were isolated from adipose tissue of human by enzymatic digestion and were differentiated into osteoblasts using osteogenic medium. Characteristics of these cells at first, second, third and fourth weeks were comprised with calvaria osteoblasts that were isolated from human calvaria by explanation culture method. To screen the characteristics of both calvaria and the differentiated osteoblasts, we used western blot to identify protein levels, von Kossa staining for mineral matrix detection and alkaline phosphatase (ALP) assay kit (Sigma) for ALP activity measurement. Difference between calvaria and differentiated osteoblast cells were analyzed by one-way ANOVA and P0 < 0.05 was considered as statistically significant. Results: Alkaline phosphatase activity, collagen and mineral material production in differentiated osteoblasts at third week were more significantly than calvaria cells ( P < 0.05). Our results indicated that there was no significant different in osteocalcin (OC) production between differentiated osteoblast at first, second and third weeks and calvaria cells but declined at fourth week (P < 0.05). Conclusions: Our survey showed that cellular traits of differentiated osteoblasts presented better than calvaria osteoblasts in vitro conditions. Therefore, we suggest that ADSCs could be used in next studies for bone tissue engineering.http://www.ijpvmjournal.net/article.asp?issn=2008-7802;year=2013;volume=4;issue=2;spage=180;epage=186;aulast=HashemibeniAdipose derived stem cellsbonecalvariaosteogenesis |
| spellingShingle | Batool Hashemibeni Farzaneh Jafary Nafiseh Esmaeil Vahid Goharian Ghader Feizi Fariba Heidari Sayyedeh Zahra Moosavi-Nejad Sayyed Hamid Zarkesh Comparison of phenotypic characterization between differentiated osteoblasts from stem cells and Calvaria osteoblasts In vitro International Journal of Preventive Medicine Adipose derived stem cells bone calvaria osteogenesis |
| title | Comparison of phenotypic characterization between differentiated osteoblasts from stem cells and Calvaria osteoblasts In vitro |
| title_full | Comparison of phenotypic characterization between differentiated osteoblasts from stem cells and Calvaria osteoblasts In vitro |
| title_fullStr | Comparison of phenotypic characterization between differentiated osteoblasts from stem cells and Calvaria osteoblasts In vitro |
| title_full_unstemmed | Comparison of phenotypic characterization between differentiated osteoblasts from stem cells and Calvaria osteoblasts In vitro |
| title_short | Comparison of phenotypic characterization between differentiated osteoblasts from stem cells and Calvaria osteoblasts In vitro |
| title_sort | comparison of phenotypic characterization between differentiated osteoblasts from stem cells and calvaria osteoblasts in vitro |
| topic | Adipose derived stem cells bone calvaria osteogenesis |
| url | http://www.ijpvmjournal.net/article.asp?issn=2008-7802;year=2013;volume=4;issue=2;spage=180;epage=186;aulast=Hashemibeni |
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