miR-29b and miR-29c are involved in Toll-like receptor control of glucocorticoid-induced apoptosis in human plasmacytoid dendritic cells.
Glucocorticoids (GCs) are frequently used to treat many of the acute disease manifestations associated with inflammatory and autoimmune disorders. However, Toll-like receptor (TLR) pathway-activated plasmacytoid dendritic cells (pDCs) are resistant to GC-induced apoptosis, which leads to the ineffic...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3720938?pdf=render |
| _version_ | 1811292422933577728 |
|---|---|
| author | Yongfeng Hong Jianxian Wu Jingpu Zhao Huiping Wang Yi Liu Tianping Chen Xiuli Kan Qianshan Tao Xianshan Shen Kaili Yan Zhimin Zhai |
| author_facet | Yongfeng Hong Jianxian Wu Jingpu Zhao Huiping Wang Yi Liu Tianping Chen Xiuli Kan Qianshan Tao Xianshan Shen Kaili Yan Zhimin Zhai |
| author_sort | Yongfeng Hong |
| collection | DOAJ |
| description | Glucocorticoids (GCs) are frequently used to treat many of the acute disease manifestations associated with inflammatory and autoimmune disorders. However, Toll-like receptor (TLR) pathway-activated plasmacytoid dendritic cells (pDCs) are resistant to GC-induced apoptosis, which leads to the inefficiency of GCs in the treatment of type I interferon-related autoimmune diseases, such as systemic lupus erythematosus (SLE). Therefore, compounds promoting pDC apoptosis may be helpful for improving the efficacy of GCs. In this study, we performed screening to identify microRNAs (miRNAs) involved in TLR-inhibited GC-induced pDC apoptosis and found an array of miRNAs that may regulate pDC apoptosis. Among those demonstrating altered expression, 6 miRNAs were inhibited in TLR-activated pDCs. Bioinformatics analysis and functional studies indicated that miR-29b and miR-29c were 2 key miRNAs involved in TLR-inhibited GC-induced pDC apoptosis. Furthermore, both of these miRNAs promoted pDC apoptosis by directly targeting Mcl-1 and Bcl-2 in human primary pDCs. Our findings provide new targets that could improve the efficacy of GCs for the treatment of SLE. |
| first_indexed | 2024-04-13T04:45:12Z |
| format | Article |
| id | doaj.art-949d085152214a23b0f6e88e4ee7c38d |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-13T04:45:12Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-949d085152214a23b0f6e88e4ee7c38d2022-12-22T03:01:52ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0187e6992610.1371/journal.pone.0069926miR-29b and miR-29c are involved in Toll-like receptor control of glucocorticoid-induced apoptosis in human plasmacytoid dendritic cells.Yongfeng HongJianxian WuJingpu ZhaoHuiping WangYi LiuTianping ChenXiuli KanQianshan TaoXianshan ShenKaili YanZhimin ZhaiGlucocorticoids (GCs) are frequently used to treat many of the acute disease manifestations associated with inflammatory and autoimmune disorders. However, Toll-like receptor (TLR) pathway-activated plasmacytoid dendritic cells (pDCs) are resistant to GC-induced apoptosis, which leads to the inefficiency of GCs in the treatment of type I interferon-related autoimmune diseases, such as systemic lupus erythematosus (SLE). Therefore, compounds promoting pDC apoptosis may be helpful for improving the efficacy of GCs. In this study, we performed screening to identify microRNAs (miRNAs) involved in TLR-inhibited GC-induced pDC apoptosis and found an array of miRNAs that may regulate pDC apoptosis. Among those demonstrating altered expression, 6 miRNAs were inhibited in TLR-activated pDCs. Bioinformatics analysis and functional studies indicated that miR-29b and miR-29c were 2 key miRNAs involved in TLR-inhibited GC-induced pDC apoptosis. Furthermore, both of these miRNAs promoted pDC apoptosis by directly targeting Mcl-1 and Bcl-2 in human primary pDCs. Our findings provide new targets that could improve the efficacy of GCs for the treatment of SLE.http://europepmc.org/articles/PMC3720938?pdf=render |
| spellingShingle | Yongfeng Hong Jianxian Wu Jingpu Zhao Huiping Wang Yi Liu Tianping Chen Xiuli Kan Qianshan Tao Xianshan Shen Kaili Yan Zhimin Zhai miR-29b and miR-29c are involved in Toll-like receptor control of glucocorticoid-induced apoptosis in human plasmacytoid dendritic cells. PLoS ONE |
| title | miR-29b and miR-29c are involved in Toll-like receptor control of glucocorticoid-induced apoptosis in human plasmacytoid dendritic cells. |
| title_full | miR-29b and miR-29c are involved in Toll-like receptor control of glucocorticoid-induced apoptosis in human plasmacytoid dendritic cells. |
| title_fullStr | miR-29b and miR-29c are involved in Toll-like receptor control of glucocorticoid-induced apoptosis in human plasmacytoid dendritic cells. |
| title_full_unstemmed | miR-29b and miR-29c are involved in Toll-like receptor control of glucocorticoid-induced apoptosis in human plasmacytoid dendritic cells. |
| title_short | miR-29b and miR-29c are involved in Toll-like receptor control of glucocorticoid-induced apoptosis in human plasmacytoid dendritic cells. |
| title_sort | mir 29b and mir 29c are involved in toll like receptor control of glucocorticoid induced apoptosis in human plasmacytoid dendritic cells |
| url | http://europepmc.org/articles/PMC3720938?pdf=render |
| work_keys_str_mv | AT yongfenghong mir29bandmir29careinvolvedintolllikereceptorcontrolofglucocorticoidinducedapoptosisinhumanplasmacytoiddendriticcells AT jianxianwu mir29bandmir29careinvolvedintolllikereceptorcontrolofglucocorticoidinducedapoptosisinhumanplasmacytoiddendriticcells AT jingpuzhao mir29bandmir29careinvolvedintolllikereceptorcontrolofglucocorticoidinducedapoptosisinhumanplasmacytoiddendriticcells AT huipingwang mir29bandmir29careinvolvedintolllikereceptorcontrolofglucocorticoidinducedapoptosisinhumanplasmacytoiddendriticcells AT yiliu mir29bandmir29careinvolvedintolllikereceptorcontrolofglucocorticoidinducedapoptosisinhumanplasmacytoiddendriticcells AT tianpingchen mir29bandmir29careinvolvedintolllikereceptorcontrolofglucocorticoidinducedapoptosisinhumanplasmacytoiddendriticcells AT xiulikan mir29bandmir29careinvolvedintolllikereceptorcontrolofglucocorticoidinducedapoptosisinhumanplasmacytoiddendriticcells AT qianshantao mir29bandmir29careinvolvedintolllikereceptorcontrolofglucocorticoidinducedapoptosisinhumanplasmacytoiddendriticcells AT xianshanshen mir29bandmir29careinvolvedintolllikereceptorcontrolofglucocorticoidinducedapoptosisinhumanplasmacytoiddendriticcells AT kailiyan mir29bandmir29careinvolvedintolllikereceptorcontrolofglucocorticoidinducedapoptosisinhumanplasmacytoiddendriticcells AT zhiminzhai mir29bandmir29careinvolvedintolllikereceptorcontrolofglucocorticoidinducedapoptosisinhumanplasmacytoiddendriticcells |