Does Chronic Pancreatitis in Growing Pigs Lead to Articular Cartilage Degradation and Alterations in Subchondral Bone?
Chronic pancreatitis (CP), a progressive inflammatory disease, poses diagnostic challenges due to its initially asymptomatic nature. While CP’s impact on exocrine and endocrine functions is well-recognized, its potential influence on other body systems, particularly in young individuals, remains und...
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MDPI AG
2024-02-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/25/4/1989 |
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| author | Ewa Tomaszewska Monika Hułas-Stasiak Piotr Dobrowolski Małgorzata Świątkiewicz Siemowit Muszyński Agnieszka Tomczyk-Warunek Tomasz Blicharski Janine Donaldson Marcin B. Arciszewski Michał Świetlicki Iwona Puzio Joanna Bonior |
| author_facet | Ewa Tomaszewska Monika Hułas-Stasiak Piotr Dobrowolski Małgorzata Świątkiewicz Siemowit Muszyński Agnieszka Tomczyk-Warunek Tomasz Blicharski Janine Donaldson Marcin B. Arciszewski Michał Świetlicki Iwona Puzio Joanna Bonior |
| author_sort | Ewa Tomaszewska |
| collection | DOAJ |
| description | Chronic pancreatitis (CP), a progressive inflammatory disease, poses diagnostic challenges due to its initially asymptomatic nature. While CP’s impact on exocrine and endocrine functions is well-recognized, its potential influence on other body systems, particularly in young individuals, remains underexplored. This study investigates the hypothesis that CP in growing pigs leads to alterations in articular cartilage and subchondral bone, potentially contributing to osteoarthritis (OA) development. Utilizing a pig model of cerulein-induced CP, we examined the structural and compositional changes in subchondral bone, articular cartilage, and synovial fluid. Histological analyses, including Picrosirius Red and Safranin-O staining, were employed alongside immuno-histochemistry and Western blotting techniques. Our findings reveal significant changes in the subchondral bone, including reduced bone volume and alterations in collagen fiber composition. Articular cartilage in CP pigs exhibited decreased proteoglycan content and alterations in key proteins such as MMP-13 and TGF-β1, indicative of early cartilage degradation. These changes suggest a link between CP and musculoskeletal alterations, underscoring the need for further research into CP’s systemic effects. Our study provides foundational insights into the relationship between CP and skeletal health, potentially guiding future pediatric healthcare strategies for early CP diagnosis and management. |
| first_indexed | 2024-03-07T22:29:52Z |
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| id | doaj.art-949e0813ec5f42f3bb787bf1ebdef33c |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-07T22:29:52Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-949e0813ec5f42f3bb787bf1ebdef33c2024-02-23T15:19:18ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672024-02-01254198910.3390/ijms25041989Does Chronic Pancreatitis in Growing Pigs Lead to Articular Cartilage Degradation and Alterations in Subchondral Bone?Ewa Tomaszewska0Monika Hułas-Stasiak1Piotr Dobrowolski2Małgorzata Świątkiewicz3Siemowit Muszyński4Agnieszka Tomczyk-Warunek5Tomasz Blicharski6Janine Donaldson7Marcin B. Arciszewski8Michał Świetlicki9Iwona Puzio10Joanna Bonior11Department of Animal Physiology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, 20-950 Lublin, PolandDepartment of Functional Anatomy and Cytobiology, Faculty of Biology and Biotechnology, Maria Curie-Sklodowska University, 20-033 Lublin, PolandDepartment of Functional Anatomy and Cytobiology, Faculty of Biology and Biotechnology, Maria Curie-Sklodowska University, 20-033 Lublin, PolandDepartment of Animal Nutrition and Feed Science, National Research Institute of Animal Production, 32-083 Balice, PolandDepartment of Biophysics, Faculty of Environmental Biology, University of Life Sciences in Lublin, 20-950 Lublin, PolandLaboratory of Locomotor System Research, Department of Rehabilitation and Physiotherapy, Medical University in Lublin, 20-090 Lublin, PolandDepartment of Orthopaedics and Rehabilitation, Medical University in Lublin, 20-090 Lublin, PolandSchool of Physiology, Faculty of Health Sciences, University of the Witwatersrand, Parktown, Johannesburg 2193, South AfricaDepartment of Animal Anatomy and Histology, University of Life Sciences in Lublin, 20-950 Lublin, PolandDepartment of Applied Physics, Faculty of Mechanical Engineering, Lublin University of Technology, 20-618 Lublin, PolandDepartment of Animal Physiology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, 20-950 Lublin, PolandDepartment of Medical Physiology, Chair of Biomedical Sciences, Institute of Physiotherapy, Faculty of Health Sciences, Jagiellonian University Medical College, 31-501 Cracow, PolandChronic pancreatitis (CP), a progressive inflammatory disease, poses diagnostic challenges due to its initially asymptomatic nature. While CP’s impact on exocrine and endocrine functions is well-recognized, its potential influence on other body systems, particularly in young individuals, remains underexplored. This study investigates the hypothesis that CP in growing pigs leads to alterations in articular cartilage and subchondral bone, potentially contributing to osteoarthritis (OA) development. Utilizing a pig model of cerulein-induced CP, we examined the structural and compositional changes in subchondral bone, articular cartilage, and synovial fluid. Histological analyses, including Picrosirius Red and Safranin-O staining, were employed alongside immuno-histochemistry and Western blotting techniques. Our findings reveal significant changes in the subchondral bone, including reduced bone volume and alterations in collagen fiber composition. Articular cartilage in CP pigs exhibited decreased proteoglycan content and alterations in key proteins such as MMP-13 and TGF-β1, indicative of early cartilage degradation. These changes suggest a link between CP and musculoskeletal alterations, underscoring the need for further research into CP’s systemic effects. Our study provides foundational insights into the relationship between CP and skeletal health, potentially guiding future pediatric healthcare strategies for early CP diagnosis and management.https://www.mdpi.com/1422-0067/25/4/1989pigchronic pancreatitisarticular cartilagecytokines |
| spellingShingle | Ewa Tomaszewska Monika Hułas-Stasiak Piotr Dobrowolski Małgorzata Świątkiewicz Siemowit Muszyński Agnieszka Tomczyk-Warunek Tomasz Blicharski Janine Donaldson Marcin B. Arciszewski Michał Świetlicki Iwona Puzio Joanna Bonior Does Chronic Pancreatitis in Growing Pigs Lead to Articular Cartilage Degradation and Alterations in Subchondral Bone? International Journal of Molecular Sciences pig chronic pancreatitis articular cartilage cytokines |
| title | Does Chronic Pancreatitis in Growing Pigs Lead to Articular Cartilage Degradation and Alterations in Subchondral Bone? |
| title_full | Does Chronic Pancreatitis in Growing Pigs Lead to Articular Cartilage Degradation and Alterations in Subchondral Bone? |
| title_fullStr | Does Chronic Pancreatitis in Growing Pigs Lead to Articular Cartilage Degradation and Alterations in Subchondral Bone? |
| title_full_unstemmed | Does Chronic Pancreatitis in Growing Pigs Lead to Articular Cartilage Degradation and Alterations in Subchondral Bone? |
| title_short | Does Chronic Pancreatitis in Growing Pigs Lead to Articular Cartilage Degradation and Alterations in Subchondral Bone? |
| title_sort | does chronic pancreatitis in growing pigs lead to articular cartilage degradation and alterations in subchondral bone |
| topic | pig chronic pancreatitis articular cartilage cytokines |
| url | https://www.mdpi.com/1422-0067/25/4/1989 |
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