Sensitive Electrochemical Biosensor for Rapid Screening of Tumor Biomarker TP53 Gene Mutation Hotspot
Rapid and sensitive detection of cancer biomarkers is crucial for cancer screening, early detection, and improving patient survival rate. The present study proposes an electrochemical gene-sensor capable of detecting tumor related TP53 gene mutation hotspots by self-assembly of sulfhydryl ended hair...
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| Format: | Article |
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MDPI AG
2022-08-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/12/8/658 |
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| author | Pengcheng Sun Kai Niu Haiying Du Ruixin Li Jiping Chen Xianbo Lu |
| author_facet | Pengcheng Sun Kai Niu Haiying Du Ruixin Li Jiping Chen Xianbo Lu |
| author_sort | Pengcheng Sun |
| collection | DOAJ |
| description | Rapid and sensitive detection of cancer biomarkers is crucial for cancer screening, early detection, and improving patient survival rate. The present study proposes an electrochemical gene-sensor capable of detecting tumor related TP53 gene mutation hotspots by self-assembly of sulfhydryl ended hairpin DNA probes tagged with methylene blue (MB) onto a gold electrode. By performing a hybridization reaction with the target DNA sequence, the gene-sensor can rearrange the probe’s structure, resulting in significant electrochemical signal differences by differential pulse voltammetry. When the DNA biosensor is hybridized with 1 μM target DNA, the peak current response signal can decrease more than 60%, displaying high sensitivity and specificity for the TP53 gene. The biosensor achieved rapid and sensitive detection of the TP53 gene with a detection limit of 10 nmol L<sup>−1</sup>, and showed good specific recognition ability for single nucleotide polymorphism (SNP) and base sequence mismatches in the TP53 gene affecting residue 248 of the P53 protein. Moreover, the biosensor demonstrated good reproducibility, repeatability, operational stability, and anti-interference ability for target DNA molecule in the complex system of 50% fetal bovine serum. The proposed biosensor provides a powerful tool for the sensitive and specific detection of TP53 gene mutation hotspot sequences and could be used in clinical samples for early diagnosis and detection of cancer. |
| first_indexed | 2024-03-09T04:39:11Z |
| format | Article |
| id | doaj.art-94a09b7decf249de87847704652f0d78 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-09T04:39:11Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-94a09b7decf249de87847704652f0d782023-12-03T13:23:22ZengMDPI AGBiosensors2079-63742022-08-0112865810.3390/bios12080658Sensitive Electrochemical Biosensor for Rapid Screening of Tumor Biomarker TP53 Gene Mutation HotspotPengcheng Sun0Kai Niu1Haiying Du2Ruixin Li3Jiping Chen4Xianbo Lu5College of Mechanical and Electronic Engineering, Dalian Minzu University, Dalian 116600, ChinaCAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, ChinaCollege of Mechanical and Electronic Engineering, Dalian Minzu University, Dalian 116600, ChinaCAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, ChinaCAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, ChinaCAS Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 457 Zhongshan Road, Dalian 116023, ChinaRapid and sensitive detection of cancer biomarkers is crucial for cancer screening, early detection, and improving patient survival rate. The present study proposes an electrochemical gene-sensor capable of detecting tumor related TP53 gene mutation hotspots by self-assembly of sulfhydryl ended hairpin DNA probes tagged with methylene blue (MB) onto a gold electrode. By performing a hybridization reaction with the target DNA sequence, the gene-sensor can rearrange the probe’s structure, resulting in significant electrochemical signal differences by differential pulse voltammetry. When the DNA biosensor is hybridized with 1 μM target DNA, the peak current response signal can decrease more than 60%, displaying high sensitivity and specificity for the TP53 gene. The biosensor achieved rapid and sensitive detection of the TP53 gene with a detection limit of 10 nmol L<sup>−1</sup>, and showed good specific recognition ability for single nucleotide polymorphism (SNP) and base sequence mismatches in the TP53 gene affecting residue 248 of the P53 protein. Moreover, the biosensor demonstrated good reproducibility, repeatability, operational stability, and anti-interference ability for target DNA molecule in the complex system of 50% fetal bovine serum. The proposed biosensor provides a powerful tool for the sensitive and specific detection of TP53 gene mutation hotspot sequences and could be used in clinical samples for early diagnosis and detection of cancer.https://www.mdpi.com/2079-6374/12/8/658biosensorcancer biomarkerelectrochemicaltumor suppressor genehairpin deoxyribonucleic acidsingle nucleotide polymorphism |
| spellingShingle | Pengcheng Sun Kai Niu Haiying Du Ruixin Li Jiping Chen Xianbo Lu Sensitive Electrochemical Biosensor for Rapid Screening of Tumor Biomarker TP53 Gene Mutation Hotspot Biosensors biosensor cancer biomarker electrochemical tumor suppressor gene hairpin deoxyribonucleic acid single nucleotide polymorphism |
| title | Sensitive Electrochemical Biosensor for Rapid Screening of Tumor Biomarker TP53 Gene Mutation Hotspot |
| title_full | Sensitive Electrochemical Biosensor for Rapid Screening of Tumor Biomarker TP53 Gene Mutation Hotspot |
| title_fullStr | Sensitive Electrochemical Biosensor for Rapid Screening of Tumor Biomarker TP53 Gene Mutation Hotspot |
| title_full_unstemmed | Sensitive Electrochemical Biosensor for Rapid Screening of Tumor Biomarker TP53 Gene Mutation Hotspot |
| title_short | Sensitive Electrochemical Biosensor for Rapid Screening of Tumor Biomarker TP53 Gene Mutation Hotspot |
| title_sort | sensitive electrochemical biosensor for rapid screening of tumor biomarker tp53 gene mutation hotspot |
| topic | biosensor cancer biomarker electrochemical tumor suppressor gene hairpin deoxyribonucleic acid single nucleotide polymorphism |
| url | https://www.mdpi.com/2079-6374/12/8/658 |
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