Adoptive cell transfer of macrophages following peripheral nerve injury in mice
Summary: Macrophages are key innate immune cells involved in multiple biological processes, including peripheral nerve regeneration. Here, we describe a protocol for the adoptive cell transfer of bone-marrow-derived macrophages (BMDMs) following sciatic nerve crush injury (SNCI). This procedure invo...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166723005919 |
| _version_ | 1797449432213487616 |
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| author | Atul Rawat Mithilesh Kumar Jha Brett Michael Morrison |
| author_facet | Atul Rawat Mithilesh Kumar Jha Brett Michael Morrison |
| author_sort | Atul Rawat |
| collection | DOAJ |
| description | Summary: Macrophages are key innate immune cells involved in multiple biological processes, including peripheral nerve regeneration. Here, we describe a protocol for the adoptive cell transfer of bone-marrow-derived macrophages (BMDMs) following sciatic nerve crush injury (SNCI). This procedure involves isolating BMDMs from a donor mouse, potentially manipulating them ex vivo, and reintroducing them into an animal following SNCI. Preclinical studies show that BMDMs can infiltrate injured nerves and impact functional recovery, potentially providing a novel therapy for nerve injuries.For complete details on the use and execution of this protocol, please refer to Jha et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-03-09T14:25:03Z |
| format | Article |
| id | doaj.art-94a17eb00ef14f60ac65ccfbaf2b6ba5 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-03-09T14:25:03Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-94a17eb00ef14f60ac65ccfbaf2b6ba52023-11-28T07:27:02ZengElsevierSTAR Protocols2666-16672023-12-0144102624Adoptive cell transfer of macrophages following peripheral nerve injury in miceAtul Rawat0Mithilesh Kumar Jha1Brett Michael Morrison2Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USADepartment of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USADepartment of Neurology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA; Corresponding authorSummary: Macrophages are key innate immune cells involved in multiple biological processes, including peripheral nerve regeneration. Here, we describe a protocol for the adoptive cell transfer of bone-marrow-derived macrophages (BMDMs) following sciatic nerve crush injury (SNCI). This procedure involves isolating BMDMs from a donor mouse, potentially manipulating them ex vivo, and reintroducing them into an animal following SNCI. Preclinical studies show that BMDMs can infiltrate injured nerves and impact functional recovery, potentially providing a novel therapy for nerve injuries.For complete details on the use and execution of this protocol, please refer to Jha et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166723005919Cell CultureImmunologyModel Organisms |
| spellingShingle | Atul Rawat Mithilesh Kumar Jha Brett Michael Morrison Adoptive cell transfer of macrophages following peripheral nerve injury in mice STAR Protocols Cell Culture Immunology Model Organisms |
| title | Adoptive cell transfer of macrophages following peripheral nerve injury in mice |
| title_full | Adoptive cell transfer of macrophages following peripheral nerve injury in mice |
| title_fullStr | Adoptive cell transfer of macrophages following peripheral nerve injury in mice |
| title_full_unstemmed | Adoptive cell transfer of macrophages following peripheral nerve injury in mice |
| title_short | Adoptive cell transfer of macrophages following peripheral nerve injury in mice |
| title_sort | adoptive cell transfer of macrophages following peripheral nerve injury in mice |
| topic | Cell Culture Immunology Model Organisms |
| url | http://www.sciencedirect.com/science/article/pii/S2666166723005919 |
| work_keys_str_mv | AT atulrawat adoptivecelltransferofmacrophagesfollowingperipheralnerveinjuryinmice AT mithileshkumarjha adoptivecelltransferofmacrophagesfollowingperipheralnerveinjuryinmice AT brettmichaelmorrison adoptivecelltransferofmacrophagesfollowingperipheralnerveinjuryinmice |