Metabolism of the Flavonol Kaempferol in Kidney Cells Liberates the B-ring to Enter Coenzyme Q Biosynthesis
Coenzyme Q (CoQ) is an essential component of the mitochondrial electron transport chain and an important antioxidant present in all cellular membranes. CoQ deficiencies are frequent in aging and in age-related diseases, and current treatments are limited to CoQ supplementation. Strategies that rely...
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MDPI AG
2020-06-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/13/2955 |
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| author | Lucía Fernández-del-Río Eric Soubeyrand Gilles J. Basset Catherine F. Clarke |
| author_facet | Lucía Fernández-del-Río Eric Soubeyrand Gilles J. Basset Catherine F. Clarke |
| author_sort | Lucía Fernández-del-Río |
| collection | DOAJ |
| description | Coenzyme Q (CoQ) is an essential component of the mitochondrial electron transport chain and an important antioxidant present in all cellular membranes. CoQ deficiencies are frequent in aging and in age-related diseases, and current treatments are limited to CoQ supplementation. Strategies that rely on CoQ supplementation suffer from poor uptake and trafficking of this very hydrophobic molecule. In a previous study, the dietary flavonol kaempferol was reported to serve as a CoQ ring precursor and to increase the CoQ content in kidney cells, but neither the part of the molecule entering CoQ biosynthesis nor the mechanism were described. In this study, kaempferol labeled specifically in the B-ring was isolated from <i>Arabidopsis</i> plants. Kidney cells treated with this compound incorporated the B-ring of kaempferol into newly synthesized CoQ, suggesting that the B-ring is metabolized via a mechanism described in plant cells. Kaempferol is a natural flavonoid present in fruits and vegetables and possesses antioxidant, anticancer, and anti-inflammatory therapeutic properties. A better understanding of the role of kaempferol as a CoQ ring precursor makes this bioactive compound a potential candidate for the design of interventions aiming to increase endogenous CoQ biosynthesis and may improve CoQ deficient phenotypes in aging and disease. |
| first_indexed | 2024-03-10T18:51:32Z |
| format | Article |
| id | doaj.art-94a229cef9bf47c7b14d4f1ca1c23a89 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T18:51:32Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-94a229cef9bf47c7b14d4f1ca1c23a892023-11-20T05:06:52ZengMDPI AGMolecules1420-30492020-06-012513295510.3390/molecules25132955Metabolism of the Flavonol Kaempferol in Kidney Cells Liberates the B-ring to Enter Coenzyme Q BiosynthesisLucía Fernández-del-Río0Eric Soubeyrand1Gilles J. Basset2Catherine F. Clarke3Department of Chemistry and Biochemistry and the Molecular Biology Institute, University of California, Los Angeles, CA 90095, USADepartment of Horticultural Sciences, University of Florida, Gainesville, FL 32611, USADepartment of Horticultural Sciences, University of Florida, Gainesville, FL 32611, USADepartment of Chemistry and Biochemistry and the Molecular Biology Institute, University of California, Los Angeles, CA 90095, USACoenzyme Q (CoQ) is an essential component of the mitochondrial electron transport chain and an important antioxidant present in all cellular membranes. CoQ deficiencies are frequent in aging and in age-related diseases, and current treatments are limited to CoQ supplementation. Strategies that rely on CoQ supplementation suffer from poor uptake and trafficking of this very hydrophobic molecule. In a previous study, the dietary flavonol kaempferol was reported to serve as a CoQ ring precursor and to increase the CoQ content in kidney cells, but neither the part of the molecule entering CoQ biosynthesis nor the mechanism were described. In this study, kaempferol labeled specifically in the B-ring was isolated from <i>Arabidopsis</i> plants. Kidney cells treated with this compound incorporated the B-ring of kaempferol into newly synthesized CoQ, suggesting that the B-ring is metabolized via a mechanism described in plant cells. Kaempferol is a natural flavonoid present in fruits and vegetables and possesses antioxidant, anticancer, and anti-inflammatory therapeutic properties. A better understanding of the role of kaempferol as a CoQ ring precursor makes this bioactive compound a potential candidate for the design of interventions aiming to increase endogenous CoQ biosynthesis and may improve CoQ deficient phenotypes in aging and disease.https://www.mdpi.com/1420-3049/25/13/2955flavonoidsflavonolkaempferolcoenzyme Qkidney cellsprecursor |
| spellingShingle | Lucía Fernández-del-Río Eric Soubeyrand Gilles J. Basset Catherine F. Clarke Metabolism of the Flavonol Kaempferol in Kidney Cells Liberates the B-ring to Enter Coenzyme Q Biosynthesis Molecules flavonoids flavonol kaempferol coenzyme Q kidney cells precursor |
| title | Metabolism of the Flavonol Kaempferol in Kidney Cells Liberates the B-ring to Enter Coenzyme Q Biosynthesis |
| title_full | Metabolism of the Flavonol Kaempferol in Kidney Cells Liberates the B-ring to Enter Coenzyme Q Biosynthesis |
| title_fullStr | Metabolism of the Flavonol Kaempferol in Kidney Cells Liberates the B-ring to Enter Coenzyme Q Biosynthesis |
| title_full_unstemmed | Metabolism of the Flavonol Kaempferol in Kidney Cells Liberates the B-ring to Enter Coenzyme Q Biosynthesis |
| title_short | Metabolism of the Flavonol Kaempferol in Kidney Cells Liberates the B-ring to Enter Coenzyme Q Biosynthesis |
| title_sort | metabolism of the flavonol kaempferol in kidney cells liberates the b ring to enter coenzyme q biosynthesis |
| topic | flavonoids flavonol kaempferol coenzyme Q kidney cells precursor |
| url | https://www.mdpi.com/1420-3049/25/13/2955 |
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