cDNA Cloning and Expression of Translationally Controlled Tumour Protein (TCTP) Isolated from Mud Crab Scylla paramamosain in Escherichia coli
Translationally controlled tumour proteins (TCTP) cDNA were cloned from the haemolymph of Scylla paramamosain by reverse transcription PCR using primers derived from Penaeus monodon. Nucleotide sequence analysis revealed 507 bp open reading frame (ORF) encoding 168 amino acid residues with a predict...
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| Format: | Article |
| Language: | English |
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Walailak University
2011-11-01
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| Series: | Walailak Journal of Science and Technology |
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| Online Access: | http://wjst.wu.ac.th/index.php/wjst/article/view/125 |
| _version_ | 1811281665340735488 |
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| author | Phuwadol BANGRAK |
| author_facet | Phuwadol BANGRAK |
| author_sort | Phuwadol BANGRAK |
| collection | DOAJ |
| description | Translationally controlled tumour proteins (TCTP) cDNA were cloned from the haemolymph of Scylla paramamosain by reverse transcription PCR using primers derived from Penaeus monodon. Nucleotide sequence analysis revealed 507 bp open reading frame (ORF) encoding 168 amino acid residues with a predicted molecular mass and pI of approximately 19.2 kDa and 4.5, respectively. Interestingly, mud crab-TCTP shows 99 % homology with Penaeus monodon translationally controlled tumour proteins (Pm-TCTP) and Fenneropenaeus merguiensis TCTP. This gene was also expressed in pQE40 vector as histidine tagged fusion proteins. The recombinant protein has a molecular mass of approximately 25 kDa. From the biological function study of Pm-TCTP, It is proposed that mud crab-TCTP might function as anti-apoptosis like Pm-TCTP does. |
| first_indexed | 2024-04-13T01:37:44Z |
| format | Article |
| id | doaj.art-94aa6f25d1544877857e4be41b3ca2f3 |
| institution | Directory Open Access Journal |
| issn | 1686-3933 2228-835X |
| language | English |
| last_indexed | 2024-04-13T01:37:44Z |
| publishDate | 2011-11-01 |
| publisher | Walailak University |
| record_format | Article |
| series | Walailak Journal of Science and Technology |
| spelling | doaj.art-94aa6f25d1544877857e4be41b3ca2f32022-12-22T03:08:17ZengWalailak UniversityWalailak Journal of Science and Technology1686-39332228-835X2011-11-014110.2004/wjst.v4i1.125118cDNA Cloning and Expression of Translationally Controlled Tumour Protein (TCTP) Isolated from Mud Crab Scylla paramamosain in Escherichia coliPhuwadol BANGRAK0School of Science and Utilization of Natural Products Research Unit, Walailak University, Nakhon Si Thammarat 80161Translationally controlled tumour proteins (TCTP) cDNA were cloned from the haemolymph of Scylla paramamosain by reverse transcription PCR using primers derived from Penaeus monodon. Nucleotide sequence analysis revealed 507 bp open reading frame (ORF) encoding 168 amino acid residues with a predicted molecular mass and pI of approximately 19.2 kDa and 4.5, respectively. Interestingly, mud crab-TCTP shows 99 % homology with Penaeus monodon translationally controlled tumour proteins (Pm-TCTP) and Fenneropenaeus merguiensis TCTP. This gene was also expressed in pQE40 vector as histidine tagged fusion proteins. The recombinant protein has a molecular mass of approximately 25 kDa. From the biological function study of Pm-TCTP, It is proposed that mud crab-TCTP might function as anti-apoptosis like Pm-TCTP does.http://wjst.wu.ac.th/index.php/wjst/article/view/125Scylla paramamosainTCTPbiodefense moleculeRT-PCR |
| spellingShingle | Phuwadol BANGRAK cDNA Cloning and Expression of Translationally Controlled Tumour Protein (TCTP) Isolated from Mud Crab Scylla paramamosain in Escherichia coli Walailak Journal of Science and Technology Scylla paramamosain TCTP biodefense molecule RT-PCR |
| title | cDNA Cloning and Expression of Translationally Controlled Tumour Protein (TCTP) Isolated from Mud Crab Scylla paramamosain in Escherichia coli |
| title_full | cDNA Cloning and Expression of Translationally Controlled Tumour Protein (TCTP) Isolated from Mud Crab Scylla paramamosain in Escherichia coli |
| title_fullStr | cDNA Cloning and Expression of Translationally Controlled Tumour Protein (TCTP) Isolated from Mud Crab Scylla paramamosain in Escherichia coli |
| title_full_unstemmed | cDNA Cloning and Expression of Translationally Controlled Tumour Protein (TCTP) Isolated from Mud Crab Scylla paramamosain in Escherichia coli |
| title_short | cDNA Cloning and Expression of Translationally Controlled Tumour Protein (TCTP) Isolated from Mud Crab Scylla paramamosain in Escherichia coli |
| title_sort | cdna cloning and expression of translationally controlled tumour protein tctp isolated from mud crab scylla paramamosain in escherichia coli |
| topic | Scylla paramamosain TCTP biodefense molecule RT-PCR |
| url | http://wjst.wu.ac.th/index.php/wjst/article/view/125 |
| work_keys_str_mv | AT phuwadolbangrak cdnacloningandexpressionoftranslationallycontrolledtumourproteintctpisolatedfrommudcrabscyllaparamamosaininescherichiacoli |