¹H-NMR Metabolic Profiling, Antioxidant Activity, and Docking Study of Common Medicinal Plant-Derived Honey
The purpose of this investigation was to determine ¹H-NMR profiling and antioxidant activity of the most common types of honey, namely, citrus honey (HC1) (<i>Morcott tangerine</i> L. and <i>Jaffa orange</i> L.), marjoram honey (HM1) (<i>Origanum majorana</i> L.),...
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MDPI AG
2022-09-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/10/1880 |
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| author | Maha Montaser Asmaa T. Ali Ahmed M. Sayed Usama Ramadan Abdelmohsen Ehab W. Zidan Raha Orfali Mostafa E. Rateb Mohamed A. Zaki Hossam M. Hassan Rabab Mohammed Mohamed S. Hifnawy |
| author_facet | Maha Montaser Asmaa T. Ali Ahmed M. Sayed Usama Ramadan Abdelmohsen Ehab W. Zidan Raha Orfali Mostafa E. Rateb Mohamed A. Zaki Hossam M. Hassan Rabab Mohammed Mohamed S. Hifnawy |
| author_sort | Maha Montaser |
| collection | DOAJ |
| description | The purpose of this investigation was to determine ¹H-NMR profiling and antioxidant activity of the most common types of honey, namely, citrus honey (HC1) (<i>Morcott tangerine</i> L. and <i>Jaffa orange</i> L.), marjoram honey (HM1) (<i>Origanum majorana</i> L.), and clover honey (HT1) (<i>Trifolium alexandrinum</i> L.), compared to their secondary metabolites (HC2, HM2, HT2, respectively). By using a ¹H-NMR-based metabolomic technique, PCA, and PLS-DA multivariate analysis, we found that HC2, HM2, HC1, and HM1 were clustered together. However, HT1 and HT2 were quite far from these and each other. This indicated that HC1, HM1, HC2, and HM2 have similar chemical compositions, while HT1 and HT2 were unique in their chemical profiles. Antioxidation potentials were determined colorimetrically for scavenging activities against DPPH, ABTS, ORAC, 5-LOX, and metal chelating activity in all honey extract samples and their secondary metabolites. Our results revealed that HC2 and HM2 possessed more antioxidant activities than HT2 in vitro. HC2 demonstrated the highest antioxidant effect in all assays, followed by HM2 (DPPH assay: IC<sub>50</sub> 2.91, 10.7 μg/mL; ABTS assay: 431.2, 210.24 at 50 ug/mL Trolox equivalent; ORAC assay: 259.5, 234.8 at 50 ug/mL Trolox equivalent; 5-LOX screening assay/IC<sub>50</sub>: 2.293, 6.136 ug/mL; and metal chelating activity at 50 ug/mL: 73.34526%, 63.75881% inhibition). We suggest that the presence of some secondary metabolites in HC and HM, such as hesperetin, linalool, and caffeic acid, increased the antioxidant activity in citrus and marjoram compared to clover honey. |
| first_indexed | 2024-03-09T20:49:44Z |
| format | Article |
| id | doaj.art-94acfb4632514ff2b4fe3b3163a2cb36 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T20:49:44Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-94acfb4632514ff2b4fe3b3163a2cb362023-11-23T22:37:14ZengMDPI AGAntioxidants2076-39212022-09-011110188010.3390/antiox11101880¹H-NMR Metabolic Profiling, Antioxidant Activity, and Docking Study of Common Medicinal Plant-Derived HoneyMaha Montaser0Asmaa T. Ali1Ahmed M. Sayed2Usama Ramadan Abdelmohsen3Ehab W. Zidan4Raha Orfali5Mostafa E. Rateb6Mohamed A. Zaki7Hossam M. Hassan8Rabab Mohammed9Mohamed S. Hifnawy10Department of Pharmacognosy, Faculty of Pharmacy, Nahda University, Beni-Suef 62513, EgyptDepartment of Biochemistry, Faculty of Pharmacy, Nahda University, Beni-Suef 62511, EgyptDepartment of Pharmacognosy, Faculty of Pharmacy, Nahda University, Beni-Suef 62513, EgyptDepartment of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia 61519, EgyptAgricultural Research Centre, Department of Bee Research, Plant Protection Research Institute, Giza 12618, EgyptDepartment of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaSchool of Computing, Engineering & Physical Sciences, University of the West of Scotland, Paisley PA1 2BE, UKDepartment of Pharmacognosy, Faculty of Pharmacy, Beni-Suef University, Beni-Suef 62514, EgyptDepartment of Pharmacognosy, Faculty of Pharmacy, Beni-Suef University, Beni-Suef 62514, EgyptDepartment of Pharmacognosy, Faculty of Pharmacy, Beni-Suef University, Beni-Suef 62514, EgyptDepartment of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo 11787, EgyptThe purpose of this investigation was to determine ¹H-NMR profiling and antioxidant activity of the most common types of honey, namely, citrus honey (HC1) (<i>Morcott tangerine</i> L. and <i>Jaffa orange</i> L.), marjoram honey (HM1) (<i>Origanum majorana</i> L.), and clover honey (HT1) (<i>Trifolium alexandrinum</i> L.), compared to their secondary metabolites (HC2, HM2, HT2, respectively). By using a ¹H-NMR-based metabolomic technique, PCA, and PLS-DA multivariate analysis, we found that HC2, HM2, HC1, and HM1 were clustered together. However, HT1 and HT2 were quite far from these and each other. This indicated that HC1, HM1, HC2, and HM2 have similar chemical compositions, while HT1 and HT2 were unique in their chemical profiles. Antioxidation potentials were determined colorimetrically for scavenging activities against DPPH, ABTS, ORAC, 5-LOX, and metal chelating activity in all honey extract samples and their secondary metabolites. Our results revealed that HC2 and HM2 possessed more antioxidant activities than HT2 in vitro. HC2 demonstrated the highest antioxidant effect in all assays, followed by HM2 (DPPH assay: IC<sub>50</sub> 2.91, 10.7 μg/mL; ABTS assay: 431.2, 210.24 at 50 ug/mL Trolox equivalent; ORAC assay: 259.5, 234.8 at 50 ug/mL Trolox equivalent; 5-LOX screening assay/IC<sub>50</sub>: 2.293, 6.136 ug/mL; and metal chelating activity at 50 ug/mL: 73.34526%, 63.75881% inhibition). We suggest that the presence of some secondary metabolites in HC and HM, such as hesperetin, linalool, and caffeic acid, increased the antioxidant activity in citrus and marjoram compared to clover honey.https://www.mdpi.com/2076-3921/11/10/1880honeyantioxidant5-LOXmetabolomicsNMRdocking |
| spellingShingle | Maha Montaser Asmaa T. Ali Ahmed M. Sayed Usama Ramadan Abdelmohsen Ehab W. Zidan Raha Orfali Mostafa E. Rateb Mohamed A. Zaki Hossam M. Hassan Rabab Mohammed Mohamed S. Hifnawy ¹H-NMR Metabolic Profiling, Antioxidant Activity, and Docking Study of Common Medicinal Plant-Derived Honey Antioxidants honey antioxidant 5-LOX metabolomics NMR docking |
| title | ¹H-NMR Metabolic Profiling, Antioxidant Activity, and Docking Study of Common Medicinal Plant-Derived Honey |
| title_full | ¹H-NMR Metabolic Profiling, Antioxidant Activity, and Docking Study of Common Medicinal Plant-Derived Honey |
| title_fullStr | ¹H-NMR Metabolic Profiling, Antioxidant Activity, and Docking Study of Common Medicinal Plant-Derived Honey |
| title_full_unstemmed | ¹H-NMR Metabolic Profiling, Antioxidant Activity, and Docking Study of Common Medicinal Plant-Derived Honey |
| title_short | ¹H-NMR Metabolic Profiling, Antioxidant Activity, and Docking Study of Common Medicinal Plant-Derived Honey |
| title_sort | ¹h nmr metabolic profiling antioxidant activity and docking study of common medicinal plant derived honey |
| topic | honey antioxidant 5-LOX metabolomics NMR docking |
| url | https://www.mdpi.com/2076-3921/11/10/1880 |
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