Fucoxanthinol from the Diatom <i>Nitzschia Laevis</i> Ameliorates Neuroinflammatory Responses in Lipopolysaccharide-Stimulated BV-2 Microglia
In recent years, microalgae have drawn increasing attention as a valuable source of functional food ingredients. Intriguingly, <i>Nitzschia laevis</i> is rich in fucoxanthinol that is seldom found in natural sources. Fucoxanthinol, a marine xanthophyll carotenoid, possesses various benef...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-02-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/18/2/116 |
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| author | Yuelian Li Lu Liu Peipei Sun Yifeng Zhang Tao Wu Han Sun Ka-Wing Cheng Feng Chen |
| author_facet | Yuelian Li Lu Liu Peipei Sun Yifeng Zhang Tao Wu Han Sun Ka-Wing Cheng Feng Chen |
| author_sort | Yuelian Li |
| collection | DOAJ |
| description | In recent years, microalgae have drawn increasing attention as a valuable source of functional food ingredients. Intriguingly, <i>Nitzschia laevis</i> is rich in fucoxanthinol that is seldom found in natural sources. Fucoxanthinol, a marine xanthophyll carotenoid, possesses various beneficial bioactivities. Nevertheless, it’s not clear whether fucoxanthinol could exert anti-neuroinflammatory function. In light of these premises, the aim of the present study was to investigate the anti-inflammatory role of fucoxanthinol purified from <i>Nitzschia laevis</i> in Lipopolysaccharide (LPS)-stimulated microglia. The results showed that pre-treatment of fucoxanthinol remarkably attenuated the expression of LPS-induced nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), and the production of tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), prostaglandin E2 (PGE-2), nitric oxide (NO) and reactive oxygen species (ROS) induction. Modulation mechanism studies revealed that fucoxanthinol hampered nuclear factor-kappa B (NF-κB), Akt, and mitogen-activated protein kinase (MAPK) pathways. Meanwhile, fucoxanthinol led to the enhancement of nuclear translocation of NF-E2-related factor 2 (Nrf2), and the upregulation of heme oxygenase-1 (HO-1) and NAD(P)H: quinone oxidoreductase 1 (NQO-1). Taken together, the results indicated that fucoxanthinol obtained from <i>Nitzschia laevis</i> had great potential as a neuroprotective agent in neuroinflammation and neurodegenerative disorders. |
| first_indexed | 2024-04-11T12:42:14Z |
| format | Article |
| id | doaj.art-94af31cde38f434b9848f9a2e0a99184 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-11T12:42:14Z |
| publishDate | 2020-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-94af31cde38f434b9848f9a2e0a991842022-12-22T04:23:28ZengMDPI AGMarine Drugs1660-33972020-02-0118211610.3390/md18020116md18020116Fucoxanthinol from the Diatom <i>Nitzschia Laevis</i> Ameliorates Neuroinflammatory Responses in Lipopolysaccharide-Stimulated BV-2 MicrogliaYuelian Li0Lu Liu1Peipei Sun2Yifeng Zhang3Tao Wu4Han Sun5Ka-Wing Cheng6Feng Chen7Institute for Food and Bioresource Engineering, College of Engineering, Peking University, Beijing 100871, ChinaInstitute for Food and Bioresource Engineering, College of Engineering, Peking University, Beijing 100871, ChinaInstitute for Food and Bioresource Engineering, College of Engineering, Peking University, Beijing 100871, ChinaInstitute for Food and Bioresource Engineering, College of Engineering, Peking University, Beijing 100871, ChinaInstitute for Food and Bioresource Engineering, College of Engineering, Peking University, Beijing 100871, ChinaInstitute for Food and Bioresource Engineering, College of Engineering, Peking University, Beijing 100871, ChinaInstitute for Advanced Study, Shenzhen University, Shenzhen 518060, ChinaInstitute for Advanced Study, Shenzhen University, Shenzhen 518060, ChinaIn recent years, microalgae have drawn increasing attention as a valuable source of functional food ingredients. Intriguingly, <i>Nitzschia laevis</i> is rich in fucoxanthinol that is seldom found in natural sources. Fucoxanthinol, a marine xanthophyll carotenoid, possesses various beneficial bioactivities. Nevertheless, it’s not clear whether fucoxanthinol could exert anti-neuroinflammatory function. In light of these premises, the aim of the present study was to investigate the anti-inflammatory role of fucoxanthinol purified from <i>Nitzschia laevis</i> in Lipopolysaccharide (LPS)-stimulated microglia. The results showed that pre-treatment of fucoxanthinol remarkably attenuated the expression of LPS-induced nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2), and the production of tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), prostaglandin E2 (PGE-2), nitric oxide (NO) and reactive oxygen species (ROS) induction. Modulation mechanism studies revealed that fucoxanthinol hampered nuclear factor-kappa B (NF-κB), Akt, and mitogen-activated protein kinase (MAPK) pathways. Meanwhile, fucoxanthinol led to the enhancement of nuclear translocation of NF-E2-related factor 2 (Nrf2), and the upregulation of heme oxygenase-1 (HO-1) and NAD(P)H: quinone oxidoreductase 1 (NQO-1). Taken together, the results indicated that fucoxanthinol obtained from <i>Nitzschia laevis</i> had great potential as a neuroprotective agent in neuroinflammation and neurodegenerative disorders.https://www.mdpi.com/1660-3397/18/2/116<i>nitzschia laevis</i>fucoxanthinolanti-inflammationmapksaktnf-κbnrf2 |
| spellingShingle | Yuelian Li Lu Liu Peipei Sun Yifeng Zhang Tao Wu Han Sun Ka-Wing Cheng Feng Chen Fucoxanthinol from the Diatom <i>Nitzschia Laevis</i> Ameliorates Neuroinflammatory Responses in Lipopolysaccharide-Stimulated BV-2 Microglia Marine Drugs <i>nitzschia laevis</i> fucoxanthinol anti-inflammation mapks akt nf-κb nrf2 |
| title | Fucoxanthinol from the Diatom <i>Nitzschia Laevis</i> Ameliorates Neuroinflammatory Responses in Lipopolysaccharide-Stimulated BV-2 Microglia |
| title_full | Fucoxanthinol from the Diatom <i>Nitzschia Laevis</i> Ameliorates Neuroinflammatory Responses in Lipopolysaccharide-Stimulated BV-2 Microglia |
| title_fullStr | Fucoxanthinol from the Diatom <i>Nitzschia Laevis</i> Ameliorates Neuroinflammatory Responses in Lipopolysaccharide-Stimulated BV-2 Microglia |
| title_full_unstemmed | Fucoxanthinol from the Diatom <i>Nitzschia Laevis</i> Ameliorates Neuroinflammatory Responses in Lipopolysaccharide-Stimulated BV-2 Microglia |
| title_short | Fucoxanthinol from the Diatom <i>Nitzschia Laevis</i> Ameliorates Neuroinflammatory Responses in Lipopolysaccharide-Stimulated BV-2 Microglia |
| title_sort | fucoxanthinol from the diatom i nitzschia laevis i ameliorates neuroinflammatory responses in lipopolysaccharide stimulated bv 2 microglia |
| topic | <i>nitzschia laevis</i> fucoxanthinol anti-inflammation mapks akt nf-κb nrf2 |
| url | https://www.mdpi.com/1660-3397/18/2/116 |
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