N1-methyladenosine methylation-related metabolic genes signature and subtypes for predicting prognosis and immune microenvironment in osteosarcoma
N1-methyladenosine methylation (m1A), as an important RNA methylation modification, regulates the development of many tumours. Metabolic reprogramming is one of the important features of tumour cells, and it plays a crucial role in tumour development and metastasis. The role of RNA methylation and m...
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2022.993594/full |
| _version_ | 1798031953034739712 |
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| author | Guowei Wang Hongyi Wang Sha Cheng Xiaobo Zhang Wanjiang Feng Pan Zhang Jianlong Wang |
| author_facet | Guowei Wang Hongyi Wang Sha Cheng Xiaobo Zhang Wanjiang Feng Pan Zhang Jianlong Wang |
| author_sort | Guowei Wang |
| collection | DOAJ |
| description | N1-methyladenosine methylation (m1A), as an important RNA methylation modification, regulates the development of many tumours. Metabolic reprogramming is one of the important features of tumour cells, and it plays a crucial role in tumour development and metastasis. The role of RNA methylation and metabolic reprogramming in osteosarcoma has been widely reported. However, the potential roles and mechanisms of m1A-related metabolic genes (MRmetabolism) in osteosarcoma have not been currently described. All of MRmetabolism were screened, then selected two MRmetabolism by least absolute shrinkage and selection operator and multifactorial regression analysis to construct a prognostic signature. Patients were divided into high-risk and low-risk groups based on the median riskscore of all patients. After randomizing patients into train and test cohorts, the reliability of the prognostic signature was validated in the whole, train and test cohort, respectively. Subsequently, based on the expression profiles of the two MRmetabolism, we performed consensus clustering to classify patients into two clusters. In addition, we explored the immune infiltration status of different risk groups and different clusters by CIBERSORT and single sample gene set enrichment analysis. Also, to better guide individualized treatment, we analyzed the immune checkpoint expression differences and drug sensitivity in the different risk groups and clusters. In conclusion, we constructed a MRmetabolism prognostic signature, which may help to assess patient prognosis, immunotherapy response. |
| first_indexed | 2024-04-11T20:06:03Z |
| format | Article |
| id | doaj.art-94af7d602dd04b3aaa9feb2151677705 |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-04-11T20:06:03Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Genetics |
| spelling | doaj.art-94af7d602dd04b3aaa9feb21516777052022-12-22T04:05:20ZengFrontiers Media S.A.Frontiers in Genetics1664-80212022-09-011310.3389/fgene.2022.993594993594N1-methyladenosine methylation-related metabolic genes signature and subtypes for predicting prognosis and immune microenvironment in osteosarcomaGuowei Wang0Hongyi Wang1Sha Cheng2Xiaobo Zhang3Wanjiang Feng4Pan Zhang5Jianlong Wang6Department of Spine Surgery, Third Xiangya Hospital, Central South University, Changsha, Hunan, ChinaMedical College, Hunan Normal University, Changsha, Hunan, ChinaDepartment of Gastroenterology, Third Xiangya Hospital, Central South University, Changsha, Hunan, ChinaDepartment of Spine Surgery, Third Xiangya Hospital, Central South University, Changsha, Hunan, ChinaDepartment of Spine Surgery, Third Xiangya Hospital, Central South University, Changsha, Hunan, ChinaDepartment of Infectious Disease, Third Xiangya Hospital, Central South University, Changsha, Hunan, ChinaDepartment of Spine Surgery, Third Xiangya Hospital, Central South University, Changsha, Hunan, ChinaN1-methyladenosine methylation (m1A), as an important RNA methylation modification, regulates the development of many tumours. Metabolic reprogramming is one of the important features of tumour cells, and it plays a crucial role in tumour development and metastasis. The role of RNA methylation and metabolic reprogramming in osteosarcoma has been widely reported. However, the potential roles and mechanisms of m1A-related metabolic genes (MRmetabolism) in osteosarcoma have not been currently described. All of MRmetabolism were screened, then selected two MRmetabolism by least absolute shrinkage and selection operator and multifactorial regression analysis to construct a prognostic signature. Patients were divided into high-risk and low-risk groups based on the median riskscore of all patients. After randomizing patients into train and test cohorts, the reliability of the prognostic signature was validated in the whole, train and test cohort, respectively. Subsequently, based on the expression profiles of the two MRmetabolism, we performed consensus clustering to classify patients into two clusters. In addition, we explored the immune infiltration status of different risk groups and different clusters by CIBERSORT and single sample gene set enrichment analysis. Also, to better guide individualized treatment, we analyzed the immune checkpoint expression differences and drug sensitivity in the different risk groups and clusters. In conclusion, we constructed a MRmetabolism prognostic signature, which may help to assess patient prognosis, immunotherapy response.https://www.frontiersin.org/articles/10.3389/fgene.2022.993594/fullN1-methyladenosine methylationmetabolismosteosarcomaimmune infiltrationimmunotherapy |
| spellingShingle | Guowei Wang Hongyi Wang Sha Cheng Xiaobo Zhang Wanjiang Feng Pan Zhang Jianlong Wang N1-methyladenosine methylation-related metabolic genes signature and subtypes for predicting prognosis and immune microenvironment in osteosarcoma Frontiers in Genetics N1-methyladenosine methylation metabolism osteosarcoma immune infiltration immunotherapy |
| title | N1-methyladenosine methylation-related metabolic genes signature and subtypes for predicting prognosis and immune microenvironment in osteosarcoma |
| title_full | N1-methyladenosine methylation-related metabolic genes signature and subtypes for predicting prognosis and immune microenvironment in osteosarcoma |
| title_fullStr | N1-methyladenosine methylation-related metabolic genes signature and subtypes for predicting prognosis and immune microenvironment in osteosarcoma |
| title_full_unstemmed | N1-methyladenosine methylation-related metabolic genes signature and subtypes for predicting prognosis and immune microenvironment in osteosarcoma |
| title_short | N1-methyladenosine methylation-related metabolic genes signature and subtypes for predicting prognosis and immune microenvironment in osteosarcoma |
| title_sort | n1 methyladenosine methylation related metabolic genes signature and subtypes for predicting prognosis and immune microenvironment in osteosarcoma |
| topic | N1-methyladenosine methylation metabolism osteosarcoma immune infiltration immunotherapy |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2022.993594/full |
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