Effect of Vitex agnus-castus ethanolic extract on hypothalamic KISS-1 gene expression in a rat model of polycystic ovary syndrome
Objective: Polycystic ovary syndrome (PCOS) is an endocrine system disruption that affects 6-10% of women. Some studies have reported the effect of Vitex agnus-castus (Vitagnus) on the hypothalamic-pituitary-gonad axis (HPG). This study was conducted to investigate Vitagnus effect on the expression...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2021-05-01
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| Series: | Avicenna Journal of Phytomedicine |
| Subjects: | |
| Online Access: | https://ajp.mums.ac.ir/article_17046_3246f9a4bd7c5d149fc733563ab3b3d2.pdf |
| _version_ | 1819105996144902144 |
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| author | Zoleykha Feizollahi Homa Mohseni Kouchesfahani Hanieh Jalali Delaram Eslimi Esfahani Abbas Sheikh Hosseini |
| author_facet | Zoleykha Feizollahi Homa Mohseni Kouchesfahani Hanieh Jalali Delaram Eslimi Esfahani Abbas Sheikh Hosseini |
| author_sort | Zoleykha Feizollahi |
| collection | DOAJ |
| description | Objective: Polycystic ovary syndrome (PCOS) is an endocrine system disruption that affects 6-10% of women. Some studies have reported the effect of Vitex agnus-castus (Vitagnus) on the hypothalamic-pituitary-gonad axis (HPG). This study was conducted to investigate Vitagnus effect on the expression of kisspeptin gene in a rat model of PCOS.
Materials and Methods: Thirty-two female rats were distributed into: control, Vitagnus-treatment (365 mg/kg for 30 days), PCOS (Letrozole for 28 days) and PCOS animals treated with Vitagnus (30 days of Vitagnus after PCOS induction). At the end of the treatments, serum and ovaries were collected for analysis. Expression level of KISS-1 gene in the hypothalamus was investigated, using Real-Time-PCR.
Results: In the PCOS group compared to control, FSH, progesterone and estradiol levels were decreased, whereas testosterone and LH levels were significantly increased. No significant changes were observed in the Vitagnus-treated animals in compare to control. However, Vitagnus treatment in the PCOS group, resulted in a raise in progesterone, estrogen and FSH levels and a reduction in the levels of testosterone and LH. Quantitative gene expression analysis showed that PCOS induction resulted in over-expression of KISS-1 gene, however, Vitagnus treatment reduced this up-regulated expression to normal level.
Conclusion: In conclusion, our results indicated that Vitagnus extract inhibited downregulation of KISS-1 gene in the hypothalamus of PCOS rats. Because of the master role of kisspeptin in adjusting the HPG axis, Vitagnus is likely to show beneficial effects in the treatment of PCOS via regulation of kisspeptin expression. This finding indicates a new aspect of Vitagnus effect and may be considered in its clinical applications. |
| first_indexed | 2024-12-22T02:31:07Z |
| format | Article |
| id | doaj.art-94b1cf6f1bfd4eed95e6c3b3b1108fc6 |
| institution | Directory Open Access Journal |
| issn | 2228-7930 2228-7949 |
| language | English |
| last_indexed | 2024-12-22T02:31:07Z |
| publishDate | 2021-05-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Avicenna Journal of Phytomedicine |
| spelling | doaj.art-94b1cf6f1bfd4eed95e6c3b3b1108fc62022-12-21T18:41:53ZengMashhad University of Medical SciencesAvicenna Journal of Phytomedicine2228-79302228-79492021-05-0111329230110.22038/ajp.2020.1704617046Effect of Vitex agnus-castus ethanolic extract on hypothalamic KISS-1 gene expression in a rat model of polycystic ovary syndromeZoleykha Feizollahi0Homa Mohseni Kouchesfahani1Hanieh Jalali2Delaram Eslimi Esfahani3Abbas Sheikh Hosseini4Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, IranDepartment of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, IranDepartment of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, IranDepartment of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, IranDepartment of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, IranObjective: Polycystic ovary syndrome (PCOS) is an endocrine system disruption that affects 6-10% of women. Some studies have reported the effect of Vitex agnus-castus (Vitagnus) on the hypothalamic-pituitary-gonad axis (HPG). This study was conducted to investigate Vitagnus effect on the expression of kisspeptin gene in a rat model of PCOS. Materials and Methods: Thirty-two female rats were distributed into: control, Vitagnus-treatment (365 mg/kg for 30 days), PCOS (Letrozole for 28 days) and PCOS animals treated with Vitagnus (30 days of Vitagnus after PCOS induction). At the end of the treatments, serum and ovaries were collected for analysis. Expression level of KISS-1 gene in the hypothalamus was investigated, using Real-Time-PCR. Results: In the PCOS group compared to control, FSH, progesterone and estradiol levels were decreased, whereas testosterone and LH levels were significantly increased. No significant changes were observed in the Vitagnus-treated animals in compare to control. However, Vitagnus treatment in the PCOS group, resulted in a raise in progesterone, estrogen and FSH levels and a reduction in the levels of testosterone and LH. Quantitative gene expression analysis showed that PCOS induction resulted in over-expression of KISS-1 gene, however, Vitagnus treatment reduced this up-regulated expression to normal level. Conclusion: In conclusion, our results indicated that Vitagnus extract inhibited downregulation of KISS-1 gene in the hypothalamus of PCOS rats. Because of the master role of kisspeptin in adjusting the HPG axis, Vitagnus is likely to show beneficial effects in the treatment of PCOS via regulation of kisspeptin expression. This finding indicates a new aspect of Vitagnus effect and may be considered in its clinical applications.https://ajp.mums.ac.ir/article_17046_3246f9a4bd7c5d149fc733563ab3b3d2.pdfkisspeptinshypothalamuspolycystic ovarian syndromevitex agnus castus extractovarian follicles |
| spellingShingle | Zoleykha Feizollahi Homa Mohseni Kouchesfahani Hanieh Jalali Delaram Eslimi Esfahani Abbas Sheikh Hosseini Effect of Vitex agnus-castus ethanolic extract on hypothalamic KISS-1 gene expression in a rat model of polycystic ovary syndrome Avicenna Journal of Phytomedicine kisspeptins hypothalamus polycystic ovarian syndrome vitex agnus castus extract ovarian follicles |
| title | Effect of Vitex agnus-castus ethanolic extract on hypothalamic KISS-1 gene expression in a rat model of polycystic ovary syndrome |
| title_full | Effect of Vitex agnus-castus ethanolic extract on hypothalamic KISS-1 gene expression in a rat model of polycystic ovary syndrome |
| title_fullStr | Effect of Vitex agnus-castus ethanolic extract on hypothalamic KISS-1 gene expression in a rat model of polycystic ovary syndrome |
| title_full_unstemmed | Effect of Vitex agnus-castus ethanolic extract on hypothalamic KISS-1 gene expression in a rat model of polycystic ovary syndrome |
| title_short | Effect of Vitex agnus-castus ethanolic extract on hypothalamic KISS-1 gene expression in a rat model of polycystic ovary syndrome |
| title_sort | effect of vitex agnus castus ethanolic extract on hypothalamic kiss 1 gene expression in a rat model of polycystic ovary syndrome |
| topic | kisspeptins hypothalamus polycystic ovarian syndrome vitex agnus castus extract ovarian follicles |
| url | https://ajp.mums.ac.ir/article_17046_3246f9a4bd7c5d149fc733563ab3b3d2.pdf |
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