Shear-Wave Elastography Using Commercially Available Ultrasound in a Mouse Model of Chronic Liver Disease
Elastography is currently used clinically to diagnose the degree of liver stiffness. We sought to develop a shear-wave elastography (SWE) measurement method using ultrasound in mice and to compare its results with those of other noninvasive tests for liver fibrosis. We divided male mice into three g...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-07-01
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| Series: | Gastrointestinal Disorders |
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| Online Access: | https://www.mdpi.com/2624-5647/4/3/15 |
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| author | Yoko Futani Megumi Hamano Riku Matsumoto Saya Hashimoto Rikuto Nishimura Mika Ueda Narumi Arihara Hideki Fujii Masafumi Ono Eiji Miyoshi Shigeyoshi Saito Yoshihiro Kamada |
| author_facet | Yoko Futani Megumi Hamano Riku Matsumoto Saya Hashimoto Rikuto Nishimura Mika Ueda Narumi Arihara Hideki Fujii Masafumi Ono Eiji Miyoshi Shigeyoshi Saito Yoshihiro Kamada |
| author_sort | Yoko Futani |
| collection | DOAJ |
| description | Elastography is currently used clinically to diagnose the degree of liver stiffness. We sought to develop a shear-wave elastography (SWE) measurement method using ultrasound in mice and to compare its results with those of other noninvasive tests for liver fibrosis. We divided male mice into three groups (normal (G1), liver fibrosis (G2), and fatty liver (G3)). We measured mouse liver SWE values and compared them with T<sub>1rho</sub> and T<sub>2</sub> values from magnetic resonance imaging results. We also compared the SWE values with the expression levels of a serum liver fibrosis biomarker (Mac-2-binding protein (M2BP)) and hepatic genes. SWE values significantly increased over time in G2 but did not change in G3. T<sub>1rho</sub> values in G2 and G3 were significantly increased compared with those in G1. T<sub>2</sub> values in G2 did not increase compared with those in group 1. T<sub>2</sub> values in G3 significantly increased compared with those in groups 1 and 2. In G2, SWE values significantly and positively correlated with T<sub>1rho</sub> values. SWE values significantly correlated with serum M2BP levels in G2 but did not correlate with inflammatory gene expression. We could measure SWE values to assess the degree of liver fibrosis in mouse models of liver disease. |
| first_indexed | 2024-03-09T23:58:33Z |
| format | Article |
| id | doaj.art-94ba32e6607847e1a1472845b7593d49 |
| institution | Directory Open Access Journal |
| issn | 2624-5647 |
| language | English |
| last_indexed | 2024-03-09T23:58:33Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Gastrointestinal Disorders |
| spelling | doaj.art-94ba32e6607847e1a1472845b7593d492023-11-23T16:21:24ZengMDPI AGGastrointestinal Disorders2624-56472022-07-014315316410.3390/gidisord4030015Shear-Wave Elastography Using Commercially Available Ultrasound in a Mouse Model of Chronic Liver DiseaseYoko Futani0Megumi Hamano1Riku Matsumoto2Saya Hashimoto3Rikuto Nishimura4Mika Ueda5Narumi Arihara6Hideki Fujii7Masafumi Ono8Eiji Miyoshi9Shigeyoshi Saito10Yoshihiro Kamada11Department of Advanced Metabolic Hepatology, Osaka University Graduate School of Medicine, Suita 565-0871, JapanDepartment of Advanced Metabolic Hepatology, Osaka University Graduate School of Medicine, Suita 565-0871, JapanDepartment of Advanced Metabolic Hepatology, Osaka University Graduate School of Medicine, Suita 565-0871, JapanDepartment of Advanced Metabolic Hepatology, Osaka University Graduate School of Medicine, Suita 565-0871, JapanDepartment of Advanced Metabolic Hepatology, Osaka University Graduate School of Medicine, Suita 565-0871, JapanDepartment of Advanced Metabolic Hepatology, Osaka University Graduate School of Medicine, Suita 565-0871, JapanDepartment of Medical Physics and Engineering, Division of Health Sciences, Osaka University Graduate School of Medicine, Suita 565-0871, JapanDepartment of Hepatology, Graduate School of Medicine, Osaka Metropolitan University, Osaka 545-8585, JapanDivision of Innovative Medicine for Hepatobiliary & Pancreatology, Faculty of Medicine, Kagawa University, Kita-gun, Kagawa 761-0793, JapanDepartment of Molecular Biochemistry & Clinical Investigation, Osaka University Graduate School of Medicine, Suita 565-0871, JapanDepartment of Medical Physics and Engineering, Division of Health Sciences, Osaka University Graduate School of Medicine, Suita 565-0871, JapanDepartment of Advanced Metabolic Hepatology, Osaka University Graduate School of Medicine, Suita 565-0871, JapanElastography is currently used clinically to diagnose the degree of liver stiffness. We sought to develop a shear-wave elastography (SWE) measurement method using ultrasound in mice and to compare its results with those of other noninvasive tests for liver fibrosis. We divided male mice into three groups (normal (G1), liver fibrosis (G2), and fatty liver (G3)). We measured mouse liver SWE values and compared them with T<sub>1rho</sub> and T<sub>2</sub> values from magnetic resonance imaging results. We also compared the SWE values with the expression levels of a serum liver fibrosis biomarker (Mac-2-binding protein (M2BP)) and hepatic genes. SWE values significantly increased over time in G2 but did not change in G3. T<sub>1rho</sub> values in G2 and G3 were significantly increased compared with those in G1. T<sub>2</sub> values in G2 did not increase compared with those in group 1. T<sub>2</sub> values in G3 significantly increased compared with those in groups 1 and 2. In G2, SWE values significantly and positively correlated with T<sub>1rho</sub> values. SWE values significantly correlated with serum M2BP levels in G2 but did not correlate with inflammatory gene expression. We could measure SWE values to assess the degree of liver fibrosis in mouse models of liver disease.https://www.mdpi.com/2624-5647/4/3/15mouse liver disease modelshear-wave elastographymagnetic resonance imagingT<sub>1rho</sub> mapT<sub>2</sub> mapnoninvasive test |
| spellingShingle | Yoko Futani Megumi Hamano Riku Matsumoto Saya Hashimoto Rikuto Nishimura Mika Ueda Narumi Arihara Hideki Fujii Masafumi Ono Eiji Miyoshi Shigeyoshi Saito Yoshihiro Kamada Shear-Wave Elastography Using Commercially Available Ultrasound in a Mouse Model of Chronic Liver Disease Gastrointestinal Disorders mouse liver disease model shear-wave elastography magnetic resonance imaging T<sub>1rho</sub> map T<sub>2</sub> map noninvasive test |
| title | Shear-Wave Elastography Using Commercially Available Ultrasound in a Mouse Model of Chronic Liver Disease |
| title_full | Shear-Wave Elastography Using Commercially Available Ultrasound in a Mouse Model of Chronic Liver Disease |
| title_fullStr | Shear-Wave Elastography Using Commercially Available Ultrasound in a Mouse Model of Chronic Liver Disease |
| title_full_unstemmed | Shear-Wave Elastography Using Commercially Available Ultrasound in a Mouse Model of Chronic Liver Disease |
| title_short | Shear-Wave Elastography Using Commercially Available Ultrasound in a Mouse Model of Chronic Liver Disease |
| title_sort | shear wave elastography using commercially available ultrasound in a mouse model of chronic liver disease |
| topic | mouse liver disease model shear-wave elastography magnetic resonance imaging T<sub>1rho</sub> map T<sub>2</sub> map noninvasive test |
| url | https://www.mdpi.com/2624-5647/4/3/15 |
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