Effect of Pregnane X Receptor on <i>CYP3A29</i> Expression in Porcine Alveolar Macrophages during <i>Mycoplasma hyopneumoniae</i> Infection
<i>Mycoplasma hyopneumoniae</i> (<i>M. hyopneumoniae</i>, <i>Mhp</i>) is the causative agent of mycoplasma pneumonia of swine (MPS). <i>M. hyopneumoniae</i> infection causes inflammation in pigs and leads to considerable economic losses in the pig indu...
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MDPI AG
2021-01-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/11/2/349 |
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| author | Xiaoyang Yang Fei Xing Li Wang Weimin Zhao Yanfeng Fu Feng Tu Bixia Li Xiaomin Fang Shouwen Ren |
| author_facet | Xiaoyang Yang Fei Xing Li Wang Weimin Zhao Yanfeng Fu Feng Tu Bixia Li Xiaomin Fang Shouwen Ren |
| author_sort | Xiaoyang Yang |
| collection | DOAJ |
| description | <i>Mycoplasma hyopneumoniae</i> (<i>M. hyopneumoniae</i>, <i>Mhp</i>) is the causative agent of mycoplasma pneumonia of swine (MPS). <i>M. hyopneumoniae</i> infection causes inflammation in pigs and leads to considerable economic losses in the pig industry. Pregnane X receptor (<i>PXR</i>) is a pluripotent gene regulatory protein that plays an important role in regulating cytochrome P-450 (CYP) in pigs in the context of inflammatory responses, drug metabolism, homeostasis, etc. We previously reported that cytochrome P450 3A29 (<i>CYP3A29</i>) expression was significantly upregulated in pigs infected with <i>M. hyopneumoniae</i> compared with healthy control pigs. This experiment mainly focused on identifying the role of <i>PXR</i> in the regulation of <i>CYP3A29</i> and inflammatory factors after <i>M. hyopneumoniae</i> infection by establishing pig alveolar macrophage (PAM) cells in which <i>PXR</i> was overexpressed or silenced. Our results showed that the overexpression of <i>PXR</i> could significantly improve the protein and the mRNA expression levels of <i>CYP3A29</i> with and without <i>M. hyopneumoniae</i> infection in PAM cells. After the expression of <i>PXR</i> was inhibited, protein and mRNA expression levels of <i>CYP3A29</i> were significantly reduced with and without <i>M. hyopneumoniae</i> infection in PAM cells. Moreover, <i>PXR</i> can regulate the mRNA expression levels of <i>IL-6</i> and <i>IL-8</i> during <i>M. hyopneumoniae</i> infection of PAM cells. In conclusion, these results suggest that <i>PXR</i> positively regulates <i>CYP3A29</i> expression during the inflammatory response caused by <i>M. hyopneumoniae</i> infection. |
| first_indexed | 2024-03-09T03:16:04Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T03:16:04Z |
| publishDate | 2021-01-01 |
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| spelling | doaj.art-94bafd46cbdf44d7b01055cbe041ecff2023-12-03T15:22:14ZengMDPI AGAnimals2076-26152021-01-0111234910.3390/ani11020349Effect of Pregnane X Receptor on <i>CYP3A29</i> Expression in Porcine Alveolar Macrophages during <i>Mycoplasma hyopneumoniae</i> InfectionXiaoyang Yang0Fei Xing1Li Wang2Weimin Zhao3Yanfeng Fu4Feng Tu5Bixia Li6Xiaomin Fang7Shouwen Ren8Institute of Animal Science, Jiangsu Academy of Agricultural Sciences/Jiangsu Germplasm Resources Protection and Utilization Platform, Nanjing 210014, ChinaInstitute of Animal Science, Jiangsu Academy of Agricultural Sciences/Jiangsu Germplasm Resources Protection and Utilization Platform, Nanjing 210014, ChinaInstitute of Animal Science, Jiangsu Academy of Agricultural Sciences/Jiangsu Germplasm Resources Protection and Utilization Platform, Nanjing 210014, ChinaInstitute of Animal Science, Jiangsu Academy of Agricultural Sciences/Jiangsu Germplasm Resources Protection and Utilization Platform, Nanjing 210014, ChinaInstitute of Animal Science, Jiangsu Academy of Agricultural Sciences/Jiangsu Germplasm Resources Protection and Utilization Platform, Nanjing 210014, ChinaInstitute of Animal Science, Jiangsu Academy of Agricultural Sciences/Jiangsu Germplasm Resources Protection and Utilization Platform, Nanjing 210014, ChinaInstitute of Animal Science, Jiangsu Academy of Agricultural Sciences/Jiangsu Germplasm Resources Protection and Utilization Platform, Nanjing 210014, ChinaInstitute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, ChinaInstitute of Animal Science, Jiangsu Academy of Agricultural Sciences/Jiangsu Germplasm Resources Protection and Utilization Platform, Nanjing 210014, China<i>Mycoplasma hyopneumoniae</i> (<i>M. hyopneumoniae</i>, <i>Mhp</i>) is the causative agent of mycoplasma pneumonia of swine (MPS). <i>M. hyopneumoniae</i> infection causes inflammation in pigs and leads to considerable economic losses in the pig industry. Pregnane X receptor (<i>PXR</i>) is a pluripotent gene regulatory protein that plays an important role in regulating cytochrome P-450 (CYP) in pigs in the context of inflammatory responses, drug metabolism, homeostasis, etc. We previously reported that cytochrome P450 3A29 (<i>CYP3A29</i>) expression was significantly upregulated in pigs infected with <i>M. hyopneumoniae</i> compared with healthy control pigs. This experiment mainly focused on identifying the role of <i>PXR</i> in the regulation of <i>CYP3A29</i> and inflammatory factors after <i>M. hyopneumoniae</i> infection by establishing pig alveolar macrophage (PAM) cells in which <i>PXR</i> was overexpressed or silenced. Our results showed that the overexpression of <i>PXR</i> could significantly improve the protein and the mRNA expression levels of <i>CYP3A29</i> with and without <i>M. hyopneumoniae</i> infection in PAM cells. After the expression of <i>PXR</i> was inhibited, protein and mRNA expression levels of <i>CYP3A29</i> were significantly reduced with and without <i>M. hyopneumoniae</i> infection in PAM cells. Moreover, <i>PXR</i> can regulate the mRNA expression levels of <i>IL-6</i> and <i>IL-8</i> during <i>M. hyopneumoniae</i> infection of PAM cells. In conclusion, these results suggest that <i>PXR</i> positively regulates <i>CYP3A29</i> expression during the inflammatory response caused by <i>M. hyopneumoniae</i> infection.https://www.mdpi.com/2076-2615/11/2/349<i>Mycoplasma hyopneumoniae</i>pregnane X receptorcytochrome P450 3A29<i>IL-6</i><i>IL-8</i> |
| spellingShingle | Xiaoyang Yang Fei Xing Li Wang Weimin Zhao Yanfeng Fu Feng Tu Bixia Li Xiaomin Fang Shouwen Ren Effect of Pregnane X Receptor on <i>CYP3A29</i> Expression in Porcine Alveolar Macrophages during <i>Mycoplasma hyopneumoniae</i> Infection Animals <i>Mycoplasma hyopneumoniae</i> pregnane X receptor cytochrome P450 3A29 <i>IL-6</i> <i>IL-8</i> |
| title | Effect of Pregnane X Receptor on <i>CYP3A29</i> Expression in Porcine Alveolar Macrophages during <i>Mycoplasma hyopneumoniae</i> Infection |
| title_full | Effect of Pregnane X Receptor on <i>CYP3A29</i> Expression in Porcine Alveolar Macrophages during <i>Mycoplasma hyopneumoniae</i> Infection |
| title_fullStr | Effect of Pregnane X Receptor on <i>CYP3A29</i> Expression in Porcine Alveolar Macrophages during <i>Mycoplasma hyopneumoniae</i> Infection |
| title_full_unstemmed | Effect of Pregnane X Receptor on <i>CYP3A29</i> Expression in Porcine Alveolar Macrophages during <i>Mycoplasma hyopneumoniae</i> Infection |
| title_short | Effect of Pregnane X Receptor on <i>CYP3A29</i> Expression in Porcine Alveolar Macrophages during <i>Mycoplasma hyopneumoniae</i> Infection |
| title_sort | effect of pregnane x receptor on i cyp3a29 i expression in porcine alveolar macrophages during i mycoplasma hyopneumoniae i infection |
| topic | <i>Mycoplasma hyopneumoniae</i> pregnane X receptor cytochrome P450 3A29 <i>IL-6</i> <i>IL-8</i> |
| url | https://www.mdpi.com/2076-2615/11/2/349 |
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