Impacts of pre‐existing diabetes mellitus on colorectal cancer in a mice model
Abstract Background Although diabetes mellitus (DM) is regarded as a risk factor of colorectal cancer (CRC), the impacts of pre‐existing DM on CRC without drug intervention remain unknown. The purpose of this study was to investigate and analyze the effects of diabetes mellitus (DM) on colorectal ca...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-05-01
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| Series: | Cancer Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/cam4.5868 |
| _version_ | 1797810438007685120 |
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| author | Yangbo Lv Shuiquan Lin Mingsheng Liu Lihui Wang Xiaoyu Wang Li Cui Jianguang Xu |
| author_facet | Yangbo Lv Shuiquan Lin Mingsheng Liu Lihui Wang Xiaoyu Wang Li Cui Jianguang Xu |
| author_sort | Yangbo Lv |
| collection | DOAJ |
| description | Abstract Background Although diabetes mellitus (DM) is regarded as a risk factor of colorectal cancer (CRC), the impacts of pre‐existing DM on CRC without drug intervention remain unknown. The purpose of this study was to investigate and analyze the effects of diabetes mellitus (DM) on colorectal cancer (CRC). And, to further explore the influencing factors and the mechanisms of DM affects CRC progression. Methods In this study, we investigated the effects of DM on CRC progression in a streptozotocin‐induced DM mice model. Furthermore, we evaluated the change of T cells levels using flow cytometry and indirect immunofluorescence. We assessed the alternation of gut microbiome and the transcriptional response using 16s rRNA sequencing and RNA‐seq. Results Results showed that the mice survival time was significantly decreased in CRC complicated with DM group (DM‐CRC), compared with only tumor bearing mice (CRC group). Furthermore, we found that DM could affect the immune response by changing the infiltration of CD4+ T cells, CD8+ T cells and mucosal‐associated invariant T cell (MAIT) in the CRC progression. In addition, DM could induce gut microbiome dysbiosis and change the transcriptional response in CRC complicated with DM. Conclusion For the first time, the effects of DM on CRC were systematically characterized in a mice model. Our findings highlight the effects of pre‐existing DM on CRC, and these findings should facilitate further studies in exploring and developing potentially targeted therapy for CRC in diabetic patients. Our results suggest that the effects induced by DM should be considered in the treatment for CRC complicated with DM patients. |
| first_indexed | 2024-03-13T07:07:46Z |
| format | Article |
| id | doaj.art-94bd8cd02cbc44f78dd65178b4760951 |
| institution | Directory Open Access Journal |
| issn | 2045-7634 |
| language | English |
| last_indexed | 2024-03-13T07:07:46Z |
| publishDate | 2023-05-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cancer Medicine |
| spelling | doaj.art-94bd8cd02cbc44f78dd65178b47609512023-06-06T07:30:47ZengWileyCancer Medicine2045-76342023-05-011210116411165010.1002/cam4.5868Impacts of pre‐existing diabetes mellitus on colorectal cancer in a mice modelYangbo Lv0Shuiquan Lin1Mingsheng Liu2Lihui Wang3Xiaoyu Wang4Li Cui5Jianguang Xu6Department of Colorectal Surgery The Quzhou Affiliated Hospital of Wenzhou Medical University, Quzhou People's Hospital Zhejiang Quzhou ChinaDepartment of Colorectal Surgery The Quzhou Affiliated Hospital of Wenzhou Medical University, Quzhou People's Hospital Zhejiang Quzhou ChinaDepartment of Colorectal Surgery The Quzhou Affiliated Hospital of Wenzhou Medical University, Quzhou People's Hospital Zhejiang Quzhou ChinaDepartment of Colorectal Surgery The Quzhou Affiliated Hospital of Wenzhou Medical University, Quzhou People's Hospital Zhejiang Quzhou ChinaShanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology Shanghai Jiao Tong University Shanghai ChinaShanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and Biology Shanghai Jiao Tong University Shanghai ChinaDepartment of Gastroenterology The Quzhou Affiliated Hospital of Wenzhou Medical University, Quzhou People's Hospital Zhejiang Quzhou ChinaAbstract Background Although diabetes mellitus (DM) is regarded as a risk factor of colorectal cancer (CRC), the impacts of pre‐existing DM on CRC without drug intervention remain unknown. The purpose of this study was to investigate and analyze the effects of diabetes mellitus (DM) on colorectal cancer (CRC). And, to further explore the influencing factors and the mechanisms of DM affects CRC progression. Methods In this study, we investigated the effects of DM on CRC progression in a streptozotocin‐induced DM mice model. Furthermore, we evaluated the change of T cells levels using flow cytometry and indirect immunofluorescence. We assessed the alternation of gut microbiome and the transcriptional response using 16s rRNA sequencing and RNA‐seq. Results Results showed that the mice survival time was significantly decreased in CRC complicated with DM group (DM‐CRC), compared with only tumor bearing mice (CRC group). Furthermore, we found that DM could affect the immune response by changing the infiltration of CD4+ T cells, CD8+ T cells and mucosal‐associated invariant T cell (MAIT) in the CRC progression. In addition, DM could induce gut microbiome dysbiosis and change the transcriptional response in CRC complicated with DM. Conclusion For the first time, the effects of DM on CRC were systematically characterized in a mice model. Our findings highlight the effects of pre‐existing DM on CRC, and these findings should facilitate further studies in exploring and developing potentially targeted therapy for CRC in diabetic patients. Our results suggest that the effects induced by DM should be considered in the treatment for CRC complicated with DM patients.https://doi.org/10.1002/cam4.5868colorectal cancerdiabetes mellitusgut microbiotaT‐cell infiltrationtranscriptome |
| spellingShingle | Yangbo Lv Shuiquan Lin Mingsheng Liu Lihui Wang Xiaoyu Wang Li Cui Jianguang Xu Impacts of pre‐existing diabetes mellitus on colorectal cancer in a mice model Cancer Medicine colorectal cancer diabetes mellitus gut microbiota T‐cell infiltration transcriptome |
| title | Impacts of pre‐existing diabetes mellitus on colorectal cancer in a mice model |
| title_full | Impacts of pre‐existing diabetes mellitus on colorectal cancer in a mice model |
| title_fullStr | Impacts of pre‐existing diabetes mellitus on colorectal cancer in a mice model |
| title_full_unstemmed | Impacts of pre‐existing diabetes mellitus on colorectal cancer in a mice model |
| title_short | Impacts of pre‐existing diabetes mellitus on colorectal cancer in a mice model |
| title_sort | impacts of pre existing diabetes mellitus on colorectal cancer in a mice model |
| topic | colorectal cancer diabetes mellitus gut microbiota T‐cell infiltration transcriptome |
| url | https://doi.org/10.1002/cam4.5868 |
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