An improved amperometric sarcosine biosensor based on graphene nanoribbon/chitosan nanocomposite for detection of prostate cancer
We describe herein an improved amperometric biosensor for detection of sarcosine, a potential biomarker for prostate cancer. The biosensor is based on covalent immobilization of sarcosine oxidase (SOx) onto nanocomposite of chitosan (CHIT) and graphene nanoribbons (GNRs) electrodeposited onto Au ele...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2022-01-01
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| Series: | Sensors International |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666351122000195 |
| _version_ | 1811177228644384768 |
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| author | Ritu Deswal Vinay Narwal Parveen Kumar Vaishali Verma Amita Suneja Dang C.S. Pundir |
| author_facet | Ritu Deswal Vinay Narwal Parveen Kumar Vaishali Verma Amita Suneja Dang C.S. Pundir |
| author_sort | Ritu Deswal |
| collection | DOAJ |
| description | We describe herein an improved amperometric biosensor for detection of sarcosine, a potential biomarker for prostate cancer. The biosensor is based on covalent immobilization of sarcosine oxidase (SOx) onto nanocomposite of chitosan (CHIT) and graphene nanoribbons (GNRs) electrodeposited onto Au electrode. The GNRs were studied by transmission electron microscopy and UV spectroscopy. The working electrode (SOx/CHIT/GNRs/AuE) exhibited maximum current at a potential of 0.1V against Ag/AgCl, generated from electrochemical oxidation of H2O2, from sarcosine by immobilized SOx. The biosensor showed optimum response i.e. current (mA) within 2s at pH 7.3 and 35 °C. There was a linearity between current (mA) and sarcosine concentration in a wider range 0.001–100 μM with a minimum detection limit of 0.001 μM and a high sensitivity of 277.5 μA/μM/cm. Analytical recoveries of added sarcosine in sera were 97.35%, within and between-batch coefficients of variation were 1.08% and 1.40% respectively. A good correlation (R2 = 0.99) was obtained between sera sarcosine values, as measured by the standard immuno kit method and present biosensor. The biosensor measured sarcosine levels in sera of prostate cancer patients, which was significantly higher than in apparently healthy persons. The enzyme electrode lost 20% of its initial activity during 180days, when stored dry at 4°C. |
| first_indexed | 2024-04-10T22:59:18Z |
| format | Article |
| id | doaj.art-94bde7309f9f4f93bae556aa540e689a |
| institution | Directory Open Access Journal |
| issn | 2666-3511 |
| language | English |
| last_indexed | 2024-04-10T22:59:18Z |
| publishDate | 2022-01-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Sensors International |
| spelling | doaj.art-94bde7309f9f4f93bae556aa540e689a2023-01-14T04:27:22ZengKeAi Communications Co., Ltd.Sensors International2666-35112022-01-013100174An improved amperometric sarcosine biosensor based on graphene nanoribbon/chitosan nanocomposite for detection of prostate cancerRitu Deswal0Vinay Narwal1Parveen Kumar2Vaishali Verma3Amita Suneja Dang4C.S. Pundir5Centre for Medical Biotechnology, M.D. University, Rohtak, 124001, Haryana, IndiaDepartment of Biochemistry, M.D. University Rohtak, 124001, Haryana, IndiaDepartment of Biochemistry, M.D. University Rohtak, 124001, Haryana, IndiaCentre for Medical Biotechnology, M.D. University, Rohtak, 124001, Haryana, IndiaCentre for Medical Biotechnology, M.D. University, Rohtak, 124001, Haryana, IndiaDepartment of Biochemistry, M.D. University Rohtak, 124001, Haryana, India; Corresponding author.We describe herein an improved amperometric biosensor for detection of sarcosine, a potential biomarker for prostate cancer. The biosensor is based on covalent immobilization of sarcosine oxidase (SOx) onto nanocomposite of chitosan (CHIT) and graphene nanoribbons (GNRs) electrodeposited onto Au electrode. The GNRs were studied by transmission electron microscopy and UV spectroscopy. The working electrode (SOx/CHIT/GNRs/AuE) exhibited maximum current at a potential of 0.1V against Ag/AgCl, generated from electrochemical oxidation of H2O2, from sarcosine by immobilized SOx. The biosensor showed optimum response i.e. current (mA) within 2s at pH 7.3 and 35 °C. There was a linearity between current (mA) and sarcosine concentration in a wider range 0.001–100 μM with a minimum detection limit of 0.001 μM and a high sensitivity of 277.5 μA/μM/cm. Analytical recoveries of added sarcosine in sera were 97.35%, within and between-batch coefficients of variation were 1.08% and 1.40% respectively. A good correlation (R2 = 0.99) was obtained between sera sarcosine values, as measured by the standard immuno kit method and present biosensor. The biosensor measured sarcosine levels in sera of prostate cancer patients, which was significantly higher than in apparently healthy persons. The enzyme electrode lost 20% of its initial activity during 180days, when stored dry at 4°C.http://www.sciencedirect.com/science/article/pii/S2666351122000195SarcosineSarcosine-biosensorGraphene-nanoribbonsChitosanSerumProstate-cancer |
| spellingShingle | Ritu Deswal Vinay Narwal Parveen Kumar Vaishali Verma Amita Suneja Dang C.S. Pundir An improved amperometric sarcosine biosensor based on graphene nanoribbon/chitosan nanocomposite for detection of prostate cancer Sensors International Sarcosine Sarcosine-biosensor Graphene-nanoribbons Chitosan Serum Prostate-cancer |
| title | An improved amperometric sarcosine biosensor based on graphene nanoribbon/chitosan nanocomposite for detection of prostate cancer |
| title_full | An improved amperometric sarcosine biosensor based on graphene nanoribbon/chitosan nanocomposite for detection of prostate cancer |
| title_fullStr | An improved amperometric sarcosine biosensor based on graphene nanoribbon/chitosan nanocomposite for detection of prostate cancer |
| title_full_unstemmed | An improved amperometric sarcosine biosensor based on graphene nanoribbon/chitosan nanocomposite for detection of prostate cancer |
| title_short | An improved amperometric sarcosine biosensor based on graphene nanoribbon/chitosan nanocomposite for detection of prostate cancer |
| title_sort | improved amperometric sarcosine biosensor based on graphene nanoribbon chitosan nanocomposite for detection of prostate cancer |
| topic | Sarcosine Sarcosine-biosensor Graphene-nanoribbons Chitosan Serum Prostate-cancer |
| url | http://www.sciencedirect.com/science/article/pii/S2666351122000195 |
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