Identification and characterization of the glucose-6-phosphate dehydrogenase gene family in the rubber tree, Hevea brasiliensis: role in latex regeneration and stress response
As the key enzyme in the pentose phosphate pathway (PPP), glucose-6-phosphate dehydrogenase (G6PDH) provides NADPH and intermediary metabolites for rubber biosynthesis, and plays an important role in plant development and stress responses. In this study, four Hevea brasiliensis (para rubber tree) Hb...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2016-02-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fpls.2016.00215/full |
| _version_ | 1819149136753065984 |
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| author | Xiangyu eLong Bin eHe Yongjun eFang Chaorong eTang |
| author_facet | Xiangyu eLong Bin eHe Yongjun eFang Chaorong eTang |
| author_sort | Xiangyu eLong |
| collection | DOAJ |
| description | As the key enzyme in the pentose phosphate pathway (PPP), glucose-6-phosphate dehydrogenase (G6PDH) provides NADPH and intermediary metabolites for rubber biosynthesis, and plays an important role in plant development and stress responses. In this study, four Hevea brasiliensis (para rubber tree) HbG6PDH genes were identified and cloned using a genome-wide scanning approach. On the basis of phylogeny analysis and subcellular localization predictions, HbG6PDH3 is a cytosolic isoform, while the other three genes (HbG6PDH1, 2 and 4) are plastidic molecular variants. Enzyme activity assay and expression pattern analysis showed HbG6PDHs to be involved in latex regeneration, and to influence rubber production positively in the rubber tree. The cytosolic HbG6PDH3 is the predominant isoform in latex, implying a principal role for this isoform in controlling carbon flow and NADPH production in the pentose phosphate pathway during latex regeneration. The expression pattern of the plastidic HbG6PDH4 correlates well with the degree of tapping panel dryness, a physiological disorder that stops the flow of latex from affected rubber trees. In addition, the four HbG6PDHs responded to temperature change and drought stress in the root, bark and leaves, implicating their possible roles in maintaining redox balance and defending against oxidative stress. |
| first_indexed | 2024-12-22T13:56:49Z |
| format | Article |
| id | doaj.art-94bdf5acf9044bf9a0b8d65dd4a2a2aa |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-22T13:56:49Z |
| publishDate | 2016-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-94bdf5acf9044bf9a0b8d65dd4a2a2aa2022-12-21T18:23:31ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2016-02-01710.3389/fpls.2016.00215168820Identification and characterization of the glucose-6-phosphate dehydrogenase gene family in the rubber tree, Hevea brasiliensis: role in latex regeneration and stress responseXiangyu eLong0Bin eHe1Yongjun eFang2Chaorong eTang3Rubber Research Institute Chinese Academy of Tropical Agricultural SciencesRubber Research Institute Chinese Academy of Tropical Agricultural SciencesRubber Research Institute Chinese Academy of Tropical Agricultural SciencesRubber Research Institute Chinese Academy of Tropical Agricultural SciencesAs the key enzyme in the pentose phosphate pathway (PPP), glucose-6-phosphate dehydrogenase (G6PDH) provides NADPH and intermediary metabolites for rubber biosynthesis, and plays an important role in plant development and stress responses. In this study, four Hevea brasiliensis (para rubber tree) HbG6PDH genes were identified and cloned using a genome-wide scanning approach. On the basis of phylogeny analysis and subcellular localization predictions, HbG6PDH3 is a cytosolic isoform, while the other three genes (HbG6PDH1, 2 and 4) are plastidic molecular variants. Enzyme activity assay and expression pattern analysis showed HbG6PDHs to be involved in latex regeneration, and to influence rubber production positively in the rubber tree. The cytosolic HbG6PDH3 is the predominant isoform in latex, implying a principal role for this isoform in controlling carbon flow and NADPH production in the pentose phosphate pathway during latex regeneration. The expression pattern of the plastidic HbG6PDH4 correlates well with the degree of tapping panel dryness, a physiological disorder that stops the flow of latex from affected rubber trees. In addition, the four HbG6PDHs responded to temperature change and drought stress in the root, bark and leaves, implicating their possible roles in maintaining redox balance and defending against oxidative stress.http://journal.frontiersin.org/Journal/10.3389/fpls.2016.00215/fullExpressionabiotic stressesHevea brasiliensisglucose-6-phosphate dehydrogenaseLatex regeneration |
| spellingShingle | Xiangyu eLong Bin eHe Yongjun eFang Chaorong eTang Identification and characterization of the glucose-6-phosphate dehydrogenase gene family in the rubber tree, Hevea brasiliensis: role in latex regeneration and stress response Frontiers in Plant Science Expression abiotic stresses Hevea brasiliensis glucose-6-phosphate dehydrogenase Latex regeneration |
| title | Identification and characterization of the glucose-6-phosphate dehydrogenase gene family in the rubber tree, Hevea brasiliensis: role in latex regeneration and stress response |
| title_full | Identification and characterization of the glucose-6-phosphate dehydrogenase gene family in the rubber tree, Hevea brasiliensis: role in latex regeneration and stress response |
| title_fullStr | Identification and characterization of the glucose-6-phosphate dehydrogenase gene family in the rubber tree, Hevea brasiliensis: role in latex regeneration and stress response |
| title_full_unstemmed | Identification and characterization of the glucose-6-phosphate dehydrogenase gene family in the rubber tree, Hevea brasiliensis: role in latex regeneration and stress response |
| title_short | Identification and characterization of the glucose-6-phosphate dehydrogenase gene family in the rubber tree, Hevea brasiliensis: role in latex regeneration and stress response |
| title_sort | identification and characterization of the glucose 6 phosphate dehydrogenase gene family in the rubber tree hevea brasiliensis role in latex regeneration and stress response |
| topic | Expression abiotic stresses Hevea brasiliensis glucose-6-phosphate dehydrogenase Latex regeneration |
| url | http://journal.frontiersin.org/Journal/10.3389/fpls.2016.00215/full |
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