Optimization of Expression Conditions, Purification and Identification of the Recombinant Cowpea Hemoglobin Lb Ⅱ in Escherichia coli
Cowpea has a red nodule containing large amounts of bean hemoglobin. The protein is a good natural pigment and can be used as color agent in artificial meat. In this study, a 456 bp sequence of cowpea leghemoglobin Ⅱ (Lb II) was obtained from the NCBI database. Using the pET-15b as the expression ve...
Main Authors: | Meng LIU, Congrui WANG, Bo LIU, Xiangzhong ZHAO |
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Format: | Article |
Language: | zho |
Published: |
The editorial department of Science and Technology of Food Industry
2023-02-01
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Series: | Shipin gongye ke-ji |
Subjects: | |
Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022050015 |
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