Tangshen formula improves diabetic nephropathy in STZ-induced diabetes rats fed with hyper-methionine by regulating the methylation status of kidney
Abstract Background The objective of this study was to examine and analyze differential methylation profiles in order to investigate the influence of hyper-methioninemia (HM) on the development of diabetic nephropathy (DN). Male Wistar rats, aged eight weeks and weighing 250–300 g, were randomly ass...
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| Format: | Article |
| Language: | English |
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BMC
2024-01-01
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| Series: | Clinical Epigenetics |
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| Online Access: | https://doi.org/10.1186/s13148-023-01620-8 |
| _version_ | 1827388417696071680 |
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| author | Yongwei Jiang GuoXiong Deng Chengyin Liu Han Tang Jing Zheng Xiaomu Kong Meimei Zhao Yi Liu Peng Gao Tianbao Li Hailing Zhao Yongtong Cao Ping Li Liang Ma |
| author_facet | Yongwei Jiang GuoXiong Deng Chengyin Liu Han Tang Jing Zheng Xiaomu Kong Meimei Zhao Yi Liu Peng Gao Tianbao Li Hailing Zhao Yongtong Cao Ping Li Liang Ma |
| author_sort | Yongwei Jiang |
| collection | DOAJ |
| description | Abstract Background The objective of this study was to examine and analyze differential methylation profiles in order to investigate the influence of hyper-methioninemia (HM) on the development of diabetic nephropathy (DN). Male Wistar rats, aged eight weeks and weighing 250–300 g, were randomly assigned into four groups: a control group (Healthy, n = 8), streptozocin-induced rats (STZ group, n = 8), HM + STZ group (n = 8), and the Tangshen Formula (TSF) treatment group (TSF group, n = 8). Blood glucose levels and other metabolic indicators were monitored before treatment and at four-week intervals until 12 weeks. Total DNA was extracted from the aforementioned groups, and DNA methylation landscapes were analyzed via reduced representative bisulfite sequencing. Results Both the STZ group and HM + STZ group exhibited increased blood glucose levels and urinary albumin/creatinine ratios in comparison with the control group. Notably, the HM + STZ group exhibited a markedly elevated urinary albumin/creatinine ratio (411.90 ± 88.86 mg/g) compared to the STZ group (238.41 ± 62.52 mg/g). TSF-treated rats demonstrated substantial reductions in both blood glucose levels and urinary albumin/creatinine ratios in comparison with the HM + STZ group. In-depth analysis of DNA methylation profiles revealed 797 genes with potential therapeutic effects related to TSF, among which approximately 2.3% had been previously reported as homologous genes. Conclusion While HM exacerbates DN through altered methylation patterns at specific CpG sites, TSF holds promise as a viable treatment for DN by restoring abnormal methylation levels. The identification of specific genes provides valuable insights into the underlying mechanisms of DN pathogenesis and offers potential therapeutic targets for further investigation. |
| first_indexed | 2024-03-08T16:17:30Z |
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| id | doaj.art-94bfd5649024458a81592fcc774f2dd3 |
| institution | Directory Open Access Journal |
| issn | 1868-7083 |
| language | English |
| last_indexed | 2024-03-08T16:17:30Z |
| publishDate | 2024-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Clinical Epigenetics |
| spelling | doaj.art-94bfd5649024458a81592fcc774f2dd32024-01-07T12:31:17ZengBMCClinical Epigenetics1868-70832024-01-0116111010.1186/s13148-023-01620-8Tangshen formula improves diabetic nephropathy in STZ-induced diabetes rats fed with hyper-methionine by regulating the methylation status of kidneyYongwei Jiang0GuoXiong Deng1Chengyin Liu2Han Tang3Jing Zheng4Xiaomu Kong5Meimei Zhao6Yi Liu7Peng Gao8Tianbao Li9Hailing Zhao10Yongtong Cao11Ping Li12Liang Ma13Clinical Laboratory, China-Japan Friendship HospitalClinical Laboratory, China-Japan Friendship HospitalBioChain (Beijing) Science and Technology Inc.BioChain (Beijing) Science and Technology Inc.Clinical Laboratory, China-Japan Friendship HospitalClinical Laboratory, China-Japan Friendship HospitalClinical Laboratory, China-Japan Friendship HospitalClinical Laboratory, China-Japan Friendship HospitalClinical Laboratory, China-Japan Friendship HospitalBioChain (Beijing) Science and Technology Inc.Beijing Key Lab Immune-Mediated Inflammatory Diseases, Institute of Clinical Medical Science, China-Japan Friendship HospitalClinical Laboratory, China-Japan Friendship HospitalBeijing Key Lab Immune-Mediated Inflammatory Diseases, Institute of Clinical Medical Science, China-Japan Friendship HospitalClinical Laboratory, China-Japan Friendship HospitalAbstract Background The objective of this study was to examine and analyze differential methylation profiles in order to investigate the influence of hyper-methioninemia (HM) on the development of diabetic nephropathy (DN). Male Wistar rats, aged eight weeks and weighing 250–300 g, were randomly assigned into four groups: a control group (Healthy, n = 8), streptozocin-induced rats (STZ group, n = 8), HM + STZ group (n = 8), and the Tangshen Formula (TSF) treatment group (TSF group, n = 8). Blood glucose levels and other metabolic indicators were monitored before treatment and at four-week intervals until 12 weeks. Total DNA was extracted from the aforementioned groups, and DNA methylation landscapes were analyzed via reduced representative bisulfite sequencing. Results Both the STZ group and HM + STZ group exhibited increased blood glucose levels and urinary albumin/creatinine ratios in comparison with the control group. Notably, the HM + STZ group exhibited a markedly elevated urinary albumin/creatinine ratio (411.90 ± 88.86 mg/g) compared to the STZ group (238.41 ± 62.52 mg/g). TSF-treated rats demonstrated substantial reductions in both blood glucose levels and urinary albumin/creatinine ratios in comparison with the HM + STZ group. In-depth analysis of DNA methylation profiles revealed 797 genes with potential therapeutic effects related to TSF, among which approximately 2.3% had been previously reported as homologous genes. Conclusion While HM exacerbates DN through altered methylation patterns at specific CpG sites, TSF holds promise as a viable treatment for DN by restoring abnormal methylation levels. The identification of specific genes provides valuable insights into the underlying mechanisms of DN pathogenesis and offers potential therapeutic targets for further investigation.https://doi.org/10.1186/s13148-023-01620-8Hyper-methionineDiabetic nephropathyDifferential methylation profilesTangshen formulaMethylation patterns |
| spellingShingle | Yongwei Jiang GuoXiong Deng Chengyin Liu Han Tang Jing Zheng Xiaomu Kong Meimei Zhao Yi Liu Peng Gao Tianbao Li Hailing Zhao Yongtong Cao Ping Li Liang Ma Tangshen formula improves diabetic nephropathy in STZ-induced diabetes rats fed with hyper-methionine by regulating the methylation status of kidney Clinical Epigenetics Hyper-methionine Diabetic nephropathy Differential methylation profiles Tangshen formula Methylation patterns |
| title | Tangshen formula improves diabetic nephropathy in STZ-induced diabetes rats fed with hyper-methionine by regulating the methylation status of kidney |
| title_full | Tangshen formula improves diabetic nephropathy in STZ-induced diabetes rats fed with hyper-methionine by regulating the methylation status of kidney |
| title_fullStr | Tangshen formula improves diabetic nephropathy in STZ-induced diabetes rats fed with hyper-methionine by regulating the methylation status of kidney |
| title_full_unstemmed | Tangshen formula improves diabetic nephropathy in STZ-induced diabetes rats fed with hyper-methionine by regulating the methylation status of kidney |
| title_short | Tangshen formula improves diabetic nephropathy in STZ-induced diabetes rats fed with hyper-methionine by regulating the methylation status of kidney |
| title_sort | tangshen formula improves diabetic nephropathy in stz induced diabetes rats fed with hyper methionine by regulating the methylation status of kidney |
| topic | Hyper-methionine Diabetic nephropathy Differential methylation profiles Tangshen formula Methylation patterns |
| url | https://doi.org/10.1186/s13148-023-01620-8 |
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