Development of a Hadal Microbial In Situ Multi-Stage Filtering and Preserving Device
The unique environment of the hadal zone has created material circulation patterns and biological gene characteristics. Microbes play an irreplaceable role in the ocean ecological environment and material circulation due to their pervasiveness, abundance, and metabolic diversity. In this paper, we d...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-12-01
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| Series: | Journal of Marine Science and Engineering |
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| Online Access: | https://www.mdpi.com/2077-1312/9/12/1424 |
| _version_ | 1797503260892856320 |
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| author | Dongrui Ruan Jiawang Chen Hao Wang Xiaoqing Peng Peng Zhou Weitao He |
| author_facet | Dongrui Ruan Jiawang Chen Hao Wang Xiaoqing Peng Peng Zhou Weitao He |
| author_sort | Dongrui Ruan |
| collection | DOAJ |
| description | The unique environment of the hadal zone has created material circulation patterns and biological gene characteristics. Microbes play an irreplaceable role in the ocean ecological environment and material circulation due to their pervasiveness, abundance, and metabolic diversity. In this paper, we designed and developed a microbial sampling device that can be used in a depth of 10,000 m, with its working parts suitable for the full-sea depth. The multi-stage membrane realized the in situ multi-stage filtrations. The samples were in situ fixedly preserved by RNAlater storage solution. At the same time, we modeled and calculated the multi-stage membrane separation and filtration process, simulated the interception phenomenon of particles with different sizes passing through the multi-stage membrane area, and explored the influence of varying inlet velocities. A multi-stage membrane separation and filtration test system was built. The operational characteristics of different filters were compared and analyzed, and the appropriate filter material was selected according to the flow capacity and physical properties. A 100 MPa high-pressure test was carried out to check the device’s performance under a high-pressure environment. The sampler prototype was constructed and tested in the Mariana Trench. The results indicated that the device could work at the deepest point of the Mariana trench. |
| first_indexed | 2024-03-10T03:48:02Z |
| format | Article |
| id | doaj.art-94c148ffb79f48db9b2a54f1a0a77559 |
| institution | Directory Open Access Journal |
| issn | 2077-1312 |
| language | English |
| last_indexed | 2024-03-10T03:48:02Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Marine Science and Engineering |
| spelling | doaj.art-94c148ffb79f48db9b2a54f1a0a775592023-11-23T09:03:34ZengMDPI AGJournal of Marine Science and Engineering2077-13122021-12-01912142410.3390/jmse9121424Development of a Hadal Microbial In Situ Multi-Stage Filtering and Preserving DeviceDongrui Ruan0Jiawang Chen1Hao Wang2Xiaoqing Peng3Peng Zhou4Weitao He5Ocean College, Zhejiang University, Zhoushan 316021, ChinaOcean College, Zhejiang University, Zhoushan 316021, ChinaOcean College, Zhejiang University, Zhoushan 316021, ChinaOcean College, Zhejiang University, Zhoushan 316021, ChinaOcean College, Zhejiang University, Zhoushan 316021, ChinaOcean College, Zhejiang University, Zhoushan 316021, ChinaThe unique environment of the hadal zone has created material circulation patterns and biological gene characteristics. Microbes play an irreplaceable role in the ocean ecological environment and material circulation due to their pervasiveness, abundance, and metabolic diversity. In this paper, we designed and developed a microbial sampling device that can be used in a depth of 10,000 m, with its working parts suitable for the full-sea depth. The multi-stage membrane realized the in situ multi-stage filtrations. The samples were in situ fixedly preserved by RNAlater storage solution. At the same time, we modeled and calculated the multi-stage membrane separation and filtration process, simulated the interception phenomenon of particles with different sizes passing through the multi-stage membrane area, and explored the influence of varying inlet velocities. A multi-stage membrane separation and filtration test system was built. The operational characteristics of different filters were compared and analyzed, and the appropriate filter material was selected according to the flow capacity and physical properties. A 100 MPa high-pressure test was carried out to check the device’s performance under a high-pressure environment. The sampler prototype was constructed and tested in the Mariana Trench. The results indicated that the device could work at the deepest point of the Mariana trench.https://www.mdpi.com/2077-1312/9/12/1424hadalin situmulti-stage membranemicroorganismshadal microbial samplers |
| spellingShingle | Dongrui Ruan Jiawang Chen Hao Wang Xiaoqing Peng Peng Zhou Weitao He Development of a Hadal Microbial In Situ Multi-Stage Filtering and Preserving Device Journal of Marine Science and Engineering hadal in situ multi-stage membrane microorganisms hadal microbial samplers |
| title | Development of a Hadal Microbial In Situ Multi-Stage Filtering and Preserving Device |
| title_full | Development of a Hadal Microbial In Situ Multi-Stage Filtering and Preserving Device |
| title_fullStr | Development of a Hadal Microbial In Situ Multi-Stage Filtering and Preserving Device |
| title_full_unstemmed | Development of a Hadal Microbial In Situ Multi-Stage Filtering and Preserving Device |
| title_short | Development of a Hadal Microbial In Situ Multi-Stage Filtering and Preserving Device |
| title_sort | development of a hadal microbial in situ multi stage filtering and preserving device |
| topic | hadal in situ multi-stage membrane microorganisms hadal microbial samplers |
| url | https://www.mdpi.com/2077-1312/9/12/1424 |
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