Molecular identification of sharks from the genus Sphyrna (Elasmobranchii: Chondrichthyes) in Maranhão Coast (Brazil)
Abstract Sharks of the genus Sphyrna are under intense exploitation globally. In Brazil’s northern coast, this genus represents a high proportion of fisheries landings and comprises four species. However, due to difficulty of specific identification when specimens are landed, most of the records are...
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| Format: | Article |
| Language: | English |
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Instituto Internacional de Ecologia
2024-03-01
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| Series: | Brazilian Journal of Biology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842024000101111&lng=en&tlng=en |
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| author | C. F. L. Sodré W. Macedo L. M. Feitosa N. S. M. Sousa R. N. F. Carvalho-Neta L. F. Carvalho Costa J. L. S. Nunes L. Tchaicka |
| author_facet | C. F. L. Sodré W. Macedo L. M. Feitosa N. S. M. Sousa R. N. F. Carvalho-Neta L. F. Carvalho Costa J. L. S. Nunes L. Tchaicka |
| author_sort | C. F. L. Sodré |
| collection | DOAJ |
| description | Abstract Sharks of the genus Sphyrna are under intense exploitation globally. In Brazil’s northern coast, this genus represents a high proportion of fisheries landings and comprises four species. However, due to difficulty of specific identification when specimens are landed, most of the records are limited to the genus level. Here we analyzed the effectiveness of ITS2 (Internal Transcribed Spacer 2 of rDNA) fragment length protocol (Abercrombie et al., 2005) for identifying hammerhead shark species, comparing with the analysis of COI (Cytochrome oxidase subunit I) and ITS2 sequences. We evaluated samples of muscle tissue acquired in the main fishing ports of Maranhão: Carutapera, Raposa e Tutóia. Sampling was conducted between March 2017 to March 2018 and complemented with material deposited in collection (2015). COI results indicated the occurrence of endangered species which are prohibited to be landed. These include Sphyrna mokarran (67%), S. lewini (15%), S. tudes (3%), and S. tiburo (15%). For the ITS2 marker, we investigated the optimization of the protocol developed by Abercrombie (2005) for to improve the use in this geographical area througout design of a new primers. |
| first_indexed | 2024-04-24T22:38:07Z |
| format | Article |
| id | doaj.art-94c2a709d95a41be88ce4898dccd3afe |
| institution | Directory Open Access Journal |
| issn | 1678-4375 |
| language | English |
| last_indexed | 2024-04-24T22:38:07Z |
| publishDate | 2024-03-01 |
| publisher | Instituto Internacional de Ecologia |
| record_format | Article |
| series | Brazilian Journal of Biology |
| spelling | doaj.art-94c2a709d95a41be88ce4898dccd3afe2024-03-19T07:38:03ZengInstituto Internacional de EcologiaBrazilian Journal of Biology1678-43752024-03-018410.1590/1519-6984.274862Molecular identification of sharks from the genus Sphyrna (Elasmobranchii: Chondrichthyes) in Maranhão Coast (Brazil)C. F. L. Sodréhttps://orcid.org/0000-0003-1346-830XW. Macedohttps://orcid.org/0009-0000-0009-4164L. M. Feitosahttps://orcid.org/0000-0002-1988-5326N. S. M. Sousahttps://orcid.org/0000-0002-0772-6598R. N. F. Carvalho-Netahttps://orcid.org/0000-0002-3519-5237L. F. Carvalho Costahttps://orcid.org/0000-0002-9590-1797J. L. S. Nuneshttps://orcid.org/0000-0001-6223-1785L. Tchaickahttps://orcid.org/0000-0003-1993-1377Abstract Sharks of the genus Sphyrna are under intense exploitation globally. In Brazil’s northern coast, this genus represents a high proportion of fisheries landings and comprises four species. However, due to difficulty of specific identification when specimens are landed, most of the records are limited to the genus level. Here we analyzed the effectiveness of ITS2 (Internal Transcribed Spacer 2 of rDNA) fragment length protocol (Abercrombie et al., 2005) for identifying hammerhead shark species, comparing with the analysis of COI (Cytochrome oxidase subunit I) and ITS2 sequences. We evaluated samples of muscle tissue acquired in the main fishing ports of Maranhão: Carutapera, Raposa e Tutóia. Sampling was conducted between March 2017 to March 2018 and complemented with material deposited in collection (2015). COI results indicated the occurrence of endangered species which are prohibited to be landed. These include Sphyrna mokarran (67%), S. lewini (15%), S. tudes (3%), and S. tiburo (15%). For the ITS2 marker, we investigated the optimization of the protocol developed by Abercrombie (2005) for to improve the use in this geographical area througout design of a new primers.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842024000101111&lng=en&tlng=enElasmobranchiiSphyrnidaeCOIITS2forensic identification |
| spellingShingle | C. F. L. Sodré W. Macedo L. M. Feitosa N. S. M. Sousa R. N. F. Carvalho-Neta L. F. Carvalho Costa J. L. S. Nunes L. Tchaicka Molecular identification of sharks from the genus Sphyrna (Elasmobranchii: Chondrichthyes) in Maranhão Coast (Brazil) Brazilian Journal of Biology Elasmobranchii Sphyrnidae COI ITS2 forensic identification |
| title | Molecular identification of sharks from the genus Sphyrna (Elasmobranchii: Chondrichthyes) in Maranhão Coast (Brazil) |
| title_full | Molecular identification of sharks from the genus Sphyrna (Elasmobranchii: Chondrichthyes) in Maranhão Coast (Brazil) |
| title_fullStr | Molecular identification of sharks from the genus Sphyrna (Elasmobranchii: Chondrichthyes) in Maranhão Coast (Brazil) |
| title_full_unstemmed | Molecular identification of sharks from the genus Sphyrna (Elasmobranchii: Chondrichthyes) in Maranhão Coast (Brazil) |
| title_short | Molecular identification of sharks from the genus Sphyrna (Elasmobranchii: Chondrichthyes) in Maranhão Coast (Brazil) |
| title_sort | molecular identification of sharks from the genus sphyrna elasmobranchii chondrichthyes in maranhao coast brazil |
| topic | Elasmobranchii Sphyrnidae COI ITS2 forensic identification |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-69842024000101111&lng=en&tlng=en |
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