SRA-domain proteins required for DRM2-mediated de novo DNA methylation.
De novo DNA methylation and the maintenance of DNA methylation in asymmetrical sequence contexts is catalyzed by homologous proteins in plants (DRM2) and animals (DNMT3a/b). In plants, targeting of DRM2 depends on small interfering RNAs (siRNAs), although the molecular details are still unclear. Her...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2008-11-01
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| Series: | PLoS Genetics |
| Online Access: | http://europepmc.org/articles/PMC2582956?pdf=render |
| _version_ | 1818290122653696000 |
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| author | Lianna M Johnson Julie A Law Anuj Khattar Ian R Henderson Steven E Jacobsen |
| author_facet | Lianna M Johnson Julie A Law Anuj Khattar Ian R Henderson Steven E Jacobsen |
| author_sort | Lianna M Johnson |
| collection | DOAJ |
| description | De novo DNA methylation and the maintenance of DNA methylation in asymmetrical sequence contexts is catalyzed by homologous proteins in plants (DRM2) and animals (DNMT3a/b). In plants, targeting of DRM2 depends on small interfering RNAs (siRNAs), although the molecular details are still unclear. Here, we show that two SRA-domain proteins (SUVH9 and SUVH2) are also essential for DRM2-mediated de novo and maintenance DNA methylation in Arabidopsis thaliana. At some loci, SUVH9 and SUVH2 act redundantly, while at other loci only SUVH2 is required, and this locus specificity correlates with the differing DNA-binding affinity of the SRA domains within SUVH9 and SUVH2. Specifically, SUVH9 preferentially binds methylated asymmetric sites, while SUVH2 preferentially binds methylated CG sites. The suvh9 and suvh2 mutations do not eliminate siRNAs, suggesting a role for SUVH9 and SUVH2 late in the RNA-directed DNA methylation pathway. With these new results, it is clear that SRA-domain proteins are involved in each of the three pathways leading to DNA methylation in Arabidopsis. |
| first_indexed | 2024-12-13T02:23:09Z |
| format | Article |
| id | doaj.art-94c427a6bc0540fda44fc76602859a6f |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-12-13T02:23:09Z |
| publishDate | 2008-11-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-94c427a6bc0540fda44fc76602859a6f2022-12-22T00:02:44ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042008-11-01411e100028010.1371/journal.pgen.1000280SRA-domain proteins required for DRM2-mediated de novo DNA methylation.Lianna M JohnsonJulie A LawAnuj KhattarIan R HendersonSteven E JacobsenDe novo DNA methylation and the maintenance of DNA methylation in asymmetrical sequence contexts is catalyzed by homologous proteins in plants (DRM2) and animals (DNMT3a/b). In plants, targeting of DRM2 depends on small interfering RNAs (siRNAs), although the molecular details are still unclear. Here, we show that two SRA-domain proteins (SUVH9 and SUVH2) are also essential for DRM2-mediated de novo and maintenance DNA methylation in Arabidopsis thaliana. At some loci, SUVH9 and SUVH2 act redundantly, while at other loci only SUVH2 is required, and this locus specificity correlates with the differing DNA-binding affinity of the SRA domains within SUVH9 and SUVH2. Specifically, SUVH9 preferentially binds methylated asymmetric sites, while SUVH2 preferentially binds methylated CG sites. The suvh9 and suvh2 mutations do not eliminate siRNAs, suggesting a role for SUVH9 and SUVH2 late in the RNA-directed DNA methylation pathway. With these new results, it is clear that SRA-domain proteins are involved in each of the three pathways leading to DNA methylation in Arabidopsis.http://europepmc.org/articles/PMC2582956?pdf=render |
| spellingShingle | Lianna M Johnson Julie A Law Anuj Khattar Ian R Henderson Steven E Jacobsen SRA-domain proteins required for DRM2-mediated de novo DNA methylation. PLoS Genetics |
| title | SRA-domain proteins required for DRM2-mediated de novo DNA methylation. |
| title_full | SRA-domain proteins required for DRM2-mediated de novo DNA methylation. |
| title_fullStr | SRA-domain proteins required for DRM2-mediated de novo DNA methylation. |
| title_full_unstemmed | SRA-domain proteins required for DRM2-mediated de novo DNA methylation. |
| title_short | SRA-domain proteins required for DRM2-mediated de novo DNA methylation. |
| title_sort | sra domain proteins required for drm2 mediated de novo dna methylation |
| url | http://europepmc.org/articles/PMC2582956?pdf=render |
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