Ex Vivo Colonic Fermentation of NUTRIOSE<sup>®</sup> Exerts Immuno-Modulatory Properties and Strong Anti-Inflammatory Effects
NUTRIOSE<sup>®</sup> (Roquette, Lestrem, France) is a resistant dextrin with well-established prebiotic effects. This study evaluated the indirect effects of pre-digested NUTRIOSE<sup>®</sup> on host immune response and gut barrier integrity. Fecal samples from eight healthy...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-09-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/15/19/4229 |
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| author | Caroline Perreau Clementine Thabuis Lynn Verstrepen Jonas Ghyselinck Massimo Marzorati |
| author_facet | Caroline Perreau Clementine Thabuis Lynn Verstrepen Jonas Ghyselinck Massimo Marzorati |
| author_sort | Caroline Perreau |
| collection | DOAJ |
| description | NUTRIOSE<sup>®</sup> (Roquette, Lestrem, France) is a resistant dextrin with well-established prebiotic effects. This study evaluated the indirect effects of pre-digested NUTRIOSE<sup>®</sup> on host immune response and gut barrier integrity. Fecal samples from eight healthy donors were inoculated in a Colon-on-a-plate<sup>®</sup> system (ProDigest, Ghent, Belgium) with or without NUTRIOSE<sup>®</sup> supplementation. Following 48 h fermentation, colonic suspensions were tested in a Caco-2/THP1-Blue™ co-culture system to determine their effects on gut barrier activity (transepithelial electrical resistance) and immune response following lipopolysaccharide stimulation. Additionally, changes in short-chain fatty acid levels (SCFA) and microbial community composition following a 48 h fermentation in the Colon-on-a-plate<sup>®</sup> system were measured. Across all donors, immune-mediated intestinal barrier damage was significantly reduced with NUTRIOSE<sup>®</sup>-supplemented colonic suspensions versus blank. Additionally, IL-6 and IL-10 levels were significantly increased, and the level of the neutrophil chemoattractant IL-8 was significantly decreased with NUTRIOSE<sup>®</sup>-supplemented colonic suspensions versus blank in the co-culture models following lipopolysaccharide stimulation. These beneficial effects of NUTRIOSE<sup>®</sup> supplementation were likely due to increased acetate and propionate levels and the enrichment of SCFA-producing bacteria. NUTRIOSE<sup>®</sup> was well fermented by the colonic bacteria of all eight donors and had protective effects on inflammation-induced disruption of the intestinal epithelial barrier and strong anti-inflammatory effects. |
| first_indexed | 2024-03-10T21:37:53Z |
| format | Article |
| id | doaj.art-94c6de74182f45668c3bc397817f5258 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T21:37:53Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-94c6de74182f45668c3bc397817f52582023-11-19T14:51:59ZengMDPI AGNutrients2072-66432023-09-011519422910.3390/nu15194229Ex Vivo Colonic Fermentation of NUTRIOSE<sup>®</sup> Exerts Immuno-Modulatory Properties and Strong Anti-Inflammatory EffectsCaroline Perreau0Clementine Thabuis1Lynn Verstrepen2Jonas Ghyselinck3Massimo Marzorati4Nutrition and Health R&D, Roquette, 1 rue de la Haute Loge, 62136 Lestrem, FranceNutrition and Health R&D, Roquette, 1 rue de la Haute Loge, 62136 Lestrem, FranceProDigest, Technologiepark 82, 9052 Zwijnaarde, BelgiumProDigest, Technologiepark 82, 9052 Zwijnaarde, BelgiumProDigest, Technologiepark 82, 9052 Zwijnaarde, BelgiumNUTRIOSE<sup>®</sup> (Roquette, Lestrem, France) is a resistant dextrin with well-established prebiotic effects. This study evaluated the indirect effects of pre-digested NUTRIOSE<sup>®</sup> on host immune response and gut barrier integrity. Fecal samples from eight healthy donors were inoculated in a Colon-on-a-plate<sup>®</sup> system (ProDigest, Ghent, Belgium) with or without NUTRIOSE<sup>®</sup> supplementation. Following 48 h fermentation, colonic suspensions were tested in a Caco-2/THP1-Blue™ co-culture system to determine their effects on gut barrier activity (transepithelial electrical resistance) and immune response following lipopolysaccharide stimulation. Additionally, changes in short-chain fatty acid levels (SCFA) and microbial community composition following a 48 h fermentation in the Colon-on-a-plate<sup>®</sup> system were measured. Across all donors, immune-mediated intestinal barrier damage was significantly reduced with NUTRIOSE<sup>®</sup>-supplemented colonic suspensions versus blank. Additionally, IL-6 and IL-10 levels were significantly increased, and the level of the neutrophil chemoattractant IL-8 was significantly decreased with NUTRIOSE<sup>®</sup>-supplemented colonic suspensions versus blank in the co-culture models following lipopolysaccharide stimulation. These beneficial effects of NUTRIOSE<sup>®</sup> supplementation were likely due to increased acetate and propionate levels and the enrichment of SCFA-producing bacteria. NUTRIOSE<sup>®</sup> was well fermented by the colonic bacteria of all eight donors and had protective effects on inflammation-induced disruption of the intestinal epithelial barrier and strong anti-inflammatory effects.https://www.mdpi.com/2072-6643/15/19/4229anti-inflammatoryColon-on-a-plateimmunomodulationprebioticresistant dextrinshort-chain fatty acid |
| spellingShingle | Caroline Perreau Clementine Thabuis Lynn Verstrepen Jonas Ghyselinck Massimo Marzorati Ex Vivo Colonic Fermentation of NUTRIOSE<sup>®</sup> Exerts Immuno-Modulatory Properties and Strong Anti-Inflammatory Effects Nutrients anti-inflammatory Colon-on-a-plate immunomodulation prebiotic resistant dextrin short-chain fatty acid |
| title | Ex Vivo Colonic Fermentation of NUTRIOSE<sup>®</sup> Exerts Immuno-Modulatory Properties and Strong Anti-Inflammatory Effects |
| title_full | Ex Vivo Colonic Fermentation of NUTRIOSE<sup>®</sup> Exerts Immuno-Modulatory Properties and Strong Anti-Inflammatory Effects |
| title_fullStr | Ex Vivo Colonic Fermentation of NUTRIOSE<sup>®</sup> Exerts Immuno-Modulatory Properties and Strong Anti-Inflammatory Effects |
| title_full_unstemmed | Ex Vivo Colonic Fermentation of NUTRIOSE<sup>®</sup> Exerts Immuno-Modulatory Properties and Strong Anti-Inflammatory Effects |
| title_short | Ex Vivo Colonic Fermentation of NUTRIOSE<sup>®</sup> Exerts Immuno-Modulatory Properties and Strong Anti-Inflammatory Effects |
| title_sort | ex vivo colonic fermentation of nutriose sup r sup exerts immuno modulatory properties and strong anti inflammatory effects |
| topic | anti-inflammatory Colon-on-a-plate immunomodulation prebiotic resistant dextrin short-chain fatty acid |
| url | https://www.mdpi.com/2072-6643/15/19/4229 |
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