Establishment of Primary Adult Skin Fibroblast Cell Lines from African Savanna Elephants (<i>Loxodonta africana</i>)
Following population declines of the African savanna elephant (<i>Loxodonta africana</i>) across the African continent, the establishment of primary cell lines of endangered wildlife species is paramount for the preservation of their genetic resources. In addition, it allows molecular an...
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MDPI AG
2023-07-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/14/2353 |
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| author | Amèlia Jansen van Vuuren Julie Bolcaen Monique Engelbrecht Willem Burger Maryna De Kock Marco Durante Randall Fisher Wilner Martínez-López Xanthene Miles Farzana Rahiman Walter Tinganelli Charlot Vandevoorde |
| author_facet | Amèlia Jansen van Vuuren Julie Bolcaen Monique Engelbrecht Willem Burger Maryna De Kock Marco Durante Randall Fisher Wilner Martínez-López Xanthene Miles Farzana Rahiman Walter Tinganelli Charlot Vandevoorde |
| author_sort | Amèlia Jansen van Vuuren |
| collection | DOAJ |
| description | Following population declines of the African savanna elephant (<i>Loxodonta africana</i>) across the African continent, the establishment of primary cell lines of endangered wildlife species is paramount for the preservation of their genetic resources. In addition, it allows molecular and functional studies on the cancer suppression mechanisms of elephants, which have previously been linked to a redundancy of tumor suppressor gene <i>TP53</i>. This methodology describes the establishment of primary elephant dermal fibroblast (EDF) cell lines from skin punch biopsy samples (diameter: ±4 mm) of African savanna elephants (<i>n</i> = 4, 14–35 years). The applied tissue collection technique is minimally invasive and paves the way for future remote biopsy darting. On average, the first explant outgrowth was observed after 15.75 ± 6.30 days. The average doubling time (Td) was 93.02 ± 16.94 h and 52.39 ± 0.46 h at passage 1 and 4, respectively. Metaphase spreads confirmed the diploid number of 56 chromosomes. The successful establishment of EDF cell lines allows for future elephant cell characterization studies and for research on the cancer resistance mechanisms of elephants, which can be harnessed for human cancer prevention and treatment and contributes to the conservation of their genetic material. |
| first_indexed | 2024-03-11T01:22:42Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T01:22:42Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-94d229ec1edc41c9983d174b18b3e95a2023-11-18T18:01:13ZengMDPI AGAnimals2076-26152023-07-011314235310.3390/ani13142353Establishment of Primary Adult Skin Fibroblast Cell Lines from African Savanna Elephants (<i>Loxodonta africana</i>)Amèlia Jansen van Vuuren0Julie Bolcaen1Monique Engelbrecht2Willem Burger3Maryna De Kock4Marco Durante5Randall Fisher6Wilner Martínez-López7Xanthene Miles8Farzana Rahiman9Walter Tinganelli10Charlot Vandevoorde11Separated Sector Cyclotron (SSC) Laboratory, Radiation Biophysics Division, National Research Foundation (NRF)-iThemba Laboratories for Accelerator Based Sciences (LABS), Cape Town 7100, South AfricaSeparated Sector Cyclotron (SSC) Laboratory, Radiation Biophysics Division, National Research Foundation (NRF)-iThemba Laboratories for Accelerator Based Sciences (LABS), Cape Town 7100, South AfricaSeparated Sector Cyclotron (SSC) Laboratory, Radiation Biophysics Division, National Research Foundation (NRF)-iThemba Laboratories for Accelerator Based Sciences (LABS), Cape Town 7100, South AfricaDr Willem Burger Consulting, Mossel Bay 6503, South AfricaDepartment of Medical Biosciences (MBS), Faculty of Natural Sciences, University of the Western Cape (UWC), Cape Town 7530, South AfricaBiophysics Department, GSI Helmholtzzentrum für Schwerionenforschung, 64291 Darmstadt, GermanySeparated Sector Cyclotron (SSC) Laboratory, Radiation Biophysics Division, National Research Foundation (NRF)-iThemba Laboratories for Accelerator Based Sciences (LABS), Cape Town 7100, South AfricaGenetics Department and Biodosimetry Service, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo 11600, UruguaySeparated Sector Cyclotron (SSC) Laboratory, Radiation Biophysics Division, National Research Foundation (NRF)-iThemba Laboratories for Accelerator Based Sciences (LABS), Cape Town 7100, South AfricaDepartment of Medical Biosciences (MBS), Faculty of Natural Sciences, University of the Western Cape (UWC), Cape Town 7530, South AfricaBiophysics Department, GSI Helmholtzzentrum für Schwerionenforschung, 64291 Darmstadt, GermanyBiophysics Department, GSI Helmholtzzentrum für Schwerionenforschung, 64291 Darmstadt, GermanyFollowing population declines of the African savanna elephant (<i>Loxodonta africana</i>) across the African continent, the establishment of primary cell lines of endangered wildlife species is paramount for the preservation of their genetic resources. In addition, it allows molecular and functional studies on the cancer suppression mechanisms of elephants, which have previously been linked to a redundancy of tumor suppressor gene <i>TP53</i>. This methodology describes the establishment of primary elephant dermal fibroblast (EDF) cell lines from skin punch biopsy samples (diameter: ±4 mm) of African savanna elephants (<i>n</i> = 4, 14–35 years). The applied tissue collection technique is minimally invasive and paves the way for future remote biopsy darting. On average, the first explant outgrowth was observed after 15.75 ± 6.30 days. The average doubling time (Td) was 93.02 ± 16.94 h and 52.39 ± 0.46 h at passage 1 and 4, respectively. Metaphase spreads confirmed the diploid number of 56 chromosomes. The successful establishment of EDF cell lines allows for future elephant cell characterization studies and for research on the cancer resistance mechanisms of elephants, which can be harnessed for human cancer prevention and treatment and contributes to the conservation of their genetic material.https://www.mdpi.com/2076-2615/13/14/2353African elephant<i>Loxodonta africana</i>dermal fibroblastsprimary cell linemetaphase spreadskin punch biopsy |
| spellingShingle | Amèlia Jansen van Vuuren Julie Bolcaen Monique Engelbrecht Willem Burger Maryna De Kock Marco Durante Randall Fisher Wilner Martínez-López Xanthene Miles Farzana Rahiman Walter Tinganelli Charlot Vandevoorde Establishment of Primary Adult Skin Fibroblast Cell Lines from African Savanna Elephants (<i>Loxodonta africana</i>) Animals African elephant <i>Loxodonta africana</i> dermal fibroblasts primary cell line metaphase spread skin punch biopsy |
| title | Establishment of Primary Adult Skin Fibroblast Cell Lines from African Savanna Elephants (<i>Loxodonta africana</i>) |
| title_full | Establishment of Primary Adult Skin Fibroblast Cell Lines from African Savanna Elephants (<i>Loxodonta africana</i>) |
| title_fullStr | Establishment of Primary Adult Skin Fibroblast Cell Lines from African Savanna Elephants (<i>Loxodonta africana</i>) |
| title_full_unstemmed | Establishment of Primary Adult Skin Fibroblast Cell Lines from African Savanna Elephants (<i>Loxodonta africana</i>) |
| title_short | Establishment of Primary Adult Skin Fibroblast Cell Lines from African Savanna Elephants (<i>Loxodonta africana</i>) |
| title_sort | establishment of primary adult skin fibroblast cell lines from african savanna elephants i loxodonta africana i |
| topic | African elephant <i>Loxodonta africana</i> dermal fibroblasts primary cell line metaphase spread skin punch biopsy |
| url | https://www.mdpi.com/2076-2615/13/14/2353 |
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