The Potassium-Dependent Transcriptome Analysis of Maize Provides Novel Insights into the Rescue Role of Auxin in Responses to Potassium Deficiency
Potassium (K<sup>+</sup>) deficiency is a key factor limiting maize growth and yield. Auxin plays an important role in maize adaptation to K<sup>+</sup> deficiency, but its physiological and molecular mechanisms are largely unclear. In this study, the exogenous application of...
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MDPI AG
2022-05-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/12/6/1318 |
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| author | Dongying Zhou Kai Wang He Zhang Qi Du Yingyan Liu Jing Wang Xiaoguang Wang Haiqiu Yu Xinhua Zhao |
| author_facet | Dongying Zhou Kai Wang He Zhang Qi Du Yingyan Liu Jing Wang Xiaoguang Wang Haiqiu Yu Xinhua Zhao |
| author_sort | Dongying Zhou |
| collection | DOAJ |
| description | Potassium (K<sup>+</sup>) deficiency is a key factor limiting maize growth and yield. Auxin plays an important role in maize adaptation to K<sup>+</sup> deficiency, but its physiological and molecular mechanisms are largely unclear. In this study, the exogenous application of 0.01 μmol·L<sup>–1</sup> α-Naphthalene acetic acid (NAA) could effectively alleviate the growth inhibition of maize roots caused by K<sup>+</sup> deficiency, especially in the low-K-sensitive maize inbred line D937. The transcriptome results showed that 3924 and 5458 genes were differentially expressed by exogenous NAA in D937 (sensitive to K<sup>+</sup> deficiency) and 90-21-3 (tolerant to K<sup>+</sup> deficiency) under K<sup>+</sup> deficiency, respectively. The exogenous application of NAA to D937 results in maintenance of the indole acetic acid (IAA) levels by inducing an upregulation in the expression of YUCCA-encoding genes and decreases abscisic acid (ABA) content by inducing the differential expression of genes encoding NCED (downregulated), ABA2 (downregulated), and PP2C (upregulated), thereby reducing growth damage caused by K<sup>+</sup> deficiency. In 90-21-3, exogenous NAA can decrease ABA content and increase IAA/ABA by inducing the differential expression of CYP707- and ABF-related genes, inhibiting the excess accumulation of reactive oxygen species by inducing the differential expression of genes encoding antioxidant enzymes, and maintain cellular K<sup>+</sup> homeostasis by regulating the expression of genes encoding K<sup>+</sup> channels and transporters, thus enhancing plant tolerance to K<sup>+</sup> deficiency. This study lays the foundation for understanding the molecular mechanisms underlying maize adaptation to K<sup>+</sup> deficiency. |
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| series | Agronomy |
| spelling | doaj.art-94d28f0dbc8a47baa27554370d0317d02023-11-23T15:09:45ZengMDPI AGAgronomy2073-43952022-05-01126131810.3390/agronomy12061318The Potassium-Dependent Transcriptome Analysis of Maize Provides Novel Insights into the Rescue Role of Auxin in Responses to Potassium DeficiencyDongying Zhou0Kai Wang1He Zhang2Qi Du3Yingyan Liu4Jing Wang5Xiaoguang Wang6Haiqiu Yu7Xinhua Zhao8College of Agronomy, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Agronomy, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Agronomy, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Agronomy, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Agronomy, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Agronomy, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Agronomy, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Agronomy, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Agronomy, Shenyang Agricultural University, Shenyang 110866, ChinaPotassium (K<sup>+</sup>) deficiency is a key factor limiting maize growth and yield. Auxin plays an important role in maize adaptation to K<sup>+</sup> deficiency, but its physiological and molecular mechanisms are largely unclear. In this study, the exogenous application of 0.01 μmol·L<sup>–1</sup> α-Naphthalene acetic acid (NAA) could effectively alleviate the growth inhibition of maize roots caused by K<sup>+</sup> deficiency, especially in the low-K-sensitive maize inbred line D937. The transcriptome results showed that 3924 and 5458 genes were differentially expressed by exogenous NAA in D937 (sensitive to K<sup>+</sup> deficiency) and 90-21-3 (tolerant to K<sup>+</sup> deficiency) under K<sup>+</sup> deficiency, respectively. The exogenous application of NAA to D937 results in maintenance of the indole acetic acid (IAA) levels by inducing an upregulation in the expression of YUCCA-encoding genes and decreases abscisic acid (ABA) content by inducing the differential expression of genes encoding NCED (downregulated), ABA2 (downregulated), and PP2C (upregulated), thereby reducing growth damage caused by K<sup>+</sup> deficiency. In 90-21-3, exogenous NAA can decrease ABA content and increase IAA/ABA by inducing the differential expression of CYP707- and ABF-related genes, inhibiting the excess accumulation of reactive oxygen species by inducing the differential expression of genes encoding antioxidant enzymes, and maintain cellular K<sup>+</sup> homeostasis by regulating the expression of genes encoding K<sup>+</sup> channels and transporters, thus enhancing plant tolerance to K<sup>+</sup> deficiency. This study lays the foundation for understanding the molecular mechanisms underlying maize adaptation to K<sup>+</sup> deficiency.https://www.mdpi.com/2073-4395/12/6/1318maizepotassium deficiencyauxinroot growthtranscriptome |
| spellingShingle | Dongying Zhou Kai Wang He Zhang Qi Du Yingyan Liu Jing Wang Xiaoguang Wang Haiqiu Yu Xinhua Zhao The Potassium-Dependent Transcriptome Analysis of Maize Provides Novel Insights into the Rescue Role of Auxin in Responses to Potassium Deficiency Agronomy maize potassium deficiency auxin root growth transcriptome |
| title | The Potassium-Dependent Transcriptome Analysis of Maize Provides Novel Insights into the Rescue Role of Auxin in Responses to Potassium Deficiency |
| title_full | The Potassium-Dependent Transcriptome Analysis of Maize Provides Novel Insights into the Rescue Role of Auxin in Responses to Potassium Deficiency |
| title_fullStr | The Potassium-Dependent Transcriptome Analysis of Maize Provides Novel Insights into the Rescue Role of Auxin in Responses to Potassium Deficiency |
| title_full_unstemmed | The Potassium-Dependent Transcriptome Analysis of Maize Provides Novel Insights into the Rescue Role of Auxin in Responses to Potassium Deficiency |
| title_short | The Potassium-Dependent Transcriptome Analysis of Maize Provides Novel Insights into the Rescue Role of Auxin in Responses to Potassium Deficiency |
| title_sort | potassium dependent transcriptome analysis of maize provides novel insights into the rescue role of auxin in responses to potassium deficiency |
| topic | maize potassium deficiency auxin root growth transcriptome |
| url | https://www.mdpi.com/2073-4395/12/6/1318 |
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