Perspectives for biocatalytic lignin utilization: cleaving 4-O-5 and Cα–Cβ bonds in dimeric lignin model compounds catalyzed by a promiscuous activity of tyrosinase
Abstract Background In the biorefinery utilizing lignocellulosic biomasses, lignin decomposition to value-added phenolic derivatives is a key issue, and recently biocatalytic delignification is emerging owing to its superior selectivity, low energy consumption, and unparalleled sustainability. Howev...
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| Format: | Article |
| Language: | English |
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BMC
2017-09-01
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| Series: | Biotechnology for Biofuels |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13068-017-0900-3 |
| _version_ | 1811340489647980544 |
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| author | Kyoungseon Min Taewoo Yum Jiye Kim Han Min Woo Yunje Kim Byoung-In Sang Young Je Yoo Yong Hwan Kim Youngsoon Um |
| author_facet | Kyoungseon Min Taewoo Yum Jiye Kim Han Min Woo Yunje Kim Byoung-In Sang Young Je Yoo Yong Hwan Kim Youngsoon Um |
| author_sort | Kyoungseon Min |
| collection | DOAJ |
| description | Abstract Background In the biorefinery utilizing lignocellulosic biomasses, lignin decomposition to value-added phenolic derivatives is a key issue, and recently biocatalytic delignification is emerging owing to its superior selectivity, low energy consumption, and unparalleled sustainability. However, besides heme-containing peroxidases and laccases, information about lignolytic biocatalysts is still limited till date. Results Herein, we report a promiscuous activity of tyrosinase which is closely associated with delignification requiring high redox potentials (>1.4 V vs. normal hydrogen electrode [NHE]). The promiscuous activity of tyrosinase not only oxidizes veratryl alcohol, a commonly used nonphenolic substrate for assaying ligninolytic activity, to veratraldehyde but also cleaves the 4-O-5 and Cα–Cβ bonds in 4-phenoxyphenol and guaiacyl glycerol-β-guaiacyl ether (GGE) that are dimeric lignin model compounds. Cyclic voltammograms additionally verified that the promiscuous activity oxidizes lignin-related high redox potential substrates. Conclusion These results might be applicable for extending the versatility of tyrosinase toward biocatalytic delignification as well as suggesting a new perspective for sustainable lignin utilization. Furthermore, the results provide insight for exploring the previously unknown promiscuous activities of biocatalysts much more diverse than ever thought before, thereby innovatively expanding the applicable area of biocatalysis. |
| first_indexed | 2024-04-13T18:42:44Z |
| format | Article |
| id | doaj.art-94d314fd65134f71b3e69ed43368c81d |
| institution | Directory Open Access Journal |
| issn | 1754-6834 |
| language | English |
| last_indexed | 2024-04-13T18:42:44Z |
| publishDate | 2017-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels |
| spelling | doaj.art-94d314fd65134f71b3e69ed43368c81d2022-12-22T02:34:40ZengBMCBiotechnology for Biofuels1754-68342017-09-011011810.1186/s13068-017-0900-3Perspectives for biocatalytic lignin utilization: cleaving 4-O-5 and Cα–Cβ bonds in dimeric lignin model compounds catalyzed by a promiscuous activity of tyrosinaseKyoungseon Min0Taewoo Yum1Jiye Kim2Han Min Woo3Yunje Kim4Byoung-In Sang5Young Je Yoo6Yong Hwan Kim7Youngsoon Um8Clean Energy Research Center, Korea Institute of Science and Technology (KIST)Clean Energy Research Center, Korea Institute of Science and Technology (KIST)Clean Energy Research Center, Korea Institute of Science and Technology (KIST)Clean Energy Research Center, Korea Institute of Science and Technology (KIST)Clean Energy Research Center, Korea Institute of Science and Technology (KIST)Department of Chemical Engineering, Hanyang UniversitySchool of Chemical and Biological Engineering, Seoul National UniversitySchool of Energy and Chemical Engineering, Ulsan National Institute of Science and Technology (UNIST)Clean Energy Research Center, Korea Institute of Science and Technology (KIST)Abstract Background In the biorefinery utilizing lignocellulosic biomasses, lignin decomposition to value-added phenolic derivatives is a key issue, and recently biocatalytic delignification is emerging owing to its superior selectivity, low energy consumption, and unparalleled sustainability. However, besides heme-containing peroxidases and laccases, information about lignolytic biocatalysts is still limited till date. Results Herein, we report a promiscuous activity of tyrosinase which is closely associated with delignification requiring high redox potentials (>1.4 V vs. normal hydrogen electrode [NHE]). The promiscuous activity of tyrosinase not only oxidizes veratryl alcohol, a commonly used nonphenolic substrate for assaying ligninolytic activity, to veratraldehyde but also cleaves the 4-O-5 and Cα–Cβ bonds in 4-phenoxyphenol and guaiacyl glycerol-β-guaiacyl ether (GGE) that are dimeric lignin model compounds. Cyclic voltammograms additionally verified that the promiscuous activity oxidizes lignin-related high redox potential substrates. Conclusion These results might be applicable for extending the versatility of tyrosinase toward biocatalytic delignification as well as suggesting a new perspective for sustainable lignin utilization. Furthermore, the results provide insight for exploring the previously unknown promiscuous activities of biocatalysts much more diverse than ever thought before, thereby innovatively expanding the applicable area of biocatalysis.http://link.springer.com/article/10.1186/s13068-017-0900-3Sustainable lignin utilizationTyrosinasePromiscuous activity4-PhenoxyphenolGuaiacyl glycerol-β-guaiacyl ether (GGE) |
| spellingShingle | Kyoungseon Min Taewoo Yum Jiye Kim Han Min Woo Yunje Kim Byoung-In Sang Young Je Yoo Yong Hwan Kim Youngsoon Um Perspectives for biocatalytic lignin utilization: cleaving 4-O-5 and Cα–Cβ bonds in dimeric lignin model compounds catalyzed by a promiscuous activity of tyrosinase Biotechnology for Biofuels Sustainable lignin utilization Tyrosinase Promiscuous activity 4-Phenoxyphenol Guaiacyl glycerol-β-guaiacyl ether (GGE) |
| title | Perspectives for biocatalytic lignin utilization: cleaving 4-O-5 and Cα–Cβ bonds in dimeric lignin model compounds catalyzed by a promiscuous activity of tyrosinase |
| title_full | Perspectives for biocatalytic lignin utilization: cleaving 4-O-5 and Cα–Cβ bonds in dimeric lignin model compounds catalyzed by a promiscuous activity of tyrosinase |
| title_fullStr | Perspectives for biocatalytic lignin utilization: cleaving 4-O-5 and Cα–Cβ bonds in dimeric lignin model compounds catalyzed by a promiscuous activity of tyrosinase |
| title_full_unstemmed | Perspectives for biocatalytic lignin utilization: cleaving 4-O-5 and Cα–Cβ bonds in dimeric lignin model compounds catalyzed by a promiscuous activity of tyrosinase |
| title_short | Perspectives for biocatalytic lignin utilization: cleaving 4-O-5 and Cα–Cβ bonds in dimeric lignin model compounds catalyzed by a promiscuous activity of tyrosinase |
| title_sort | perspectives for biocatalytic lignin utilization cleaving 4 o 5 and cα cβ bonds in dimeric lignin model compounds catalyzed by a promiscuous activity of tyrosinase |
| topic | Sustainable lignin utilization Tyrosinase Promiscuous activity 4-Phenoxyphenol Guaiacyl glycerol-β-guaiacyl ether (GGE) |
| url | http://link.springer.com/article/10.1186/s13068-017-0900-3 |
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