Effects of active, inactive, and derivatives of Akkermansia muciniphila on the expression of the endocannabinoid system and PPARs genes
Abstract This study aimed to investigate the effects of active and heat-inactivated forms of Akkermansia muciniphila, bacterium-derived outer membrane vesicles (OMVs), and cell-free supernatant on the transcription of endocannabinoid system (ECS) members, including cannabinoid receptors 1 and 2 (CB1...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-13840-8 |
| _version_ | 1818236848636428288 |
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| author | Farinaz Ghaderi Fattah Sotoodehnejadnematalahi Zahra Hajebrahimi Abolfazl Fateh Seyed Davar Siadat |
| author_facet | Farinaz Ghaderi Fattah Sotoodehnejadnematalahi Zahra Hajebrahimi Abolfazl Fateh Seyed Davar Siadat |
| author_sort | Farinaz Ghaderi |
| collection | DOAJ |
| description | Abstract This study aimed to investigate the effects of active and heat-inactivated forms of Akkermansia muciniphila, bacterium-derived outer membrane vesicles (OMVs), and cell-free supernatant on the transcription of endocannabinoid system (ECS) members, including cannabinoid receptors 1 and 2 (CB1 and CB2), fatty acid amide hydrolase (FAAH), and peroxisome proliferator-activated receptors (PPARs) genes (i.e., α, β/δ, and δ) in Caco-2 and HepG-2 cell lines. After the inoculation of A. muciniphila in brain heart infusion enriched medium, OMVs and cell-free supernatant were extracted. For the investigation of the effects of bacteria and its derivatives on the expression of ECS and PPARs genes, the aforementioned cells were treated by active and heat-inactivated bacteria, OMVs, and cell-free supernatant. Quantitative real-time polymerase chain reaction analysis revealed that both forms of the bacterium, bacterial-derived OMVs, and cell-free supernatant could affect the expression of CB1, CB2, FAAH, and PPARs genes (i.e., α, β/δ, and δ) significantly (P < 0.05). Considering the engagement of the aforementioned genes in metabolic pathways, it might be suggested that both forms of the bacterium, OMVs, and cell-free supernatant might have the potential to serve as a probiotic, paraprobiotic, and postbiotic candidate to prevent obesity, metabolic disorders, and liver diseases. |
| first_indexed | 2024-12-12T12:16:23Z |
| format | Article |
| id | doaj.art-94d3318ff68a496b90c020df8735c805 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-12T12:16:23Z |
| publishDate | 2022-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-94d3318ff68a496b90c020df8735c8052022-12-22T00:24:45ZengNature PortfolioScientific Reports2045-23222022-06-0112111210.1038/s41598-022-13840-8Effects of active, inactive, and derivatives of Akkermansia muciniphila on the expression of the endocannabinoid system and PPARs genesFarinaz Ghaderi0Fattah Sotoodehnejadnematalahi1Zahra Hajebrahimi2Abolfazl Fateh3Seyed Davar Siadat4Department of Biology, Science and Research Branch, Islamic Azad UniversityDepartment of Biology, Science and Research Branch, Islamic Azad UniversityA&S Research Institute, Ministry of Science Research and TechnologyDepartment of Mycobacteriology and Pulmonary Research and Microbiology Research Center (MRC), Pasteur Institute of IranDepartment of Mycobacteriology and Pulmonary Research and Microbiology Research Center (MRC), Pasteur Institute of IranAbstract This study aimed to investigate the effects of active and heat-inactivated forms of Akkermansia muciniphila, bacterium-derived outer membrane vesicles (OMVs), and cell-free supernatant on the transcription of endocannabinoid system (ECS) members, including cannabinoid receptors 1 and 2 (CB1 and CB2), fatty acid amide hydrolase (FAAH), and peroxisome proliferator-activated receptors (PPARs) genes (i.e., α, β/δ, and δ) in Caco-2 and HepG-2 cell lines. After the inoculation of A. muciniphila in brain heart infusion enriched medium, OMVs and cell-free supernatant were extracted. For the investigation of the effects of bacteria and its derivatives on the expression of ECS and PPARs genes, the aforementioned cells were treated by active and heat-inactivated bacteria, OMVs, and cell-free supernatant. Quantitative real-time polymerase chain reaction analysis revealed that both forms of the bacterium, bacterial-derived OMVs, and cell-free supernatant could affect the expression of CB1, CB2, FAAH, and PPARs genes (i.e., α, β/δ, and δ) significantly (P < 0.05). Considering the engagement of the aforementioned genes in metabolic pathways, it might be suggested that both forms of the bacterium, OMVs, and cell-free supernatant might have the potential to serve as a probiotic, paraprobiotic, and postbiotic candidate to prevent obesity, metabolic disorders, and liver diseases.https://doi.org/10.1038/s41598-022-13840-8 |
| spellingShingle | Farinaz Ghaderi Fattah Sotoodehnejadnematalahi Zahra Hajebrahimi Abolfazl Fateh Seyed Davar Siadat Effects of active, inactive, and derivatives of Akkermansia muciniphila on the expression of the endocannabinoid system and PPARs genes Scientific Reports |
| title | Effects of active, inactive, and derivatives of Akkermansia muciniphila on the expression of the endocannabinoid system and PPARs genes |
| title_full | Effects of active, inactive, and derivatives of Akkermansia muciniphila on the expression of the endocannabinoid system and PPARs genes |
| title_fullStr | Effects of active, inactive, and derivatives of Akkermansia muciniphila on the expression of the endocannabinoid system and PPARs genes |
| title_full_unstemmed | Effects of active, inactive, and derivatives of Akkermansia muciniphila on the expression of the endocannabinoid system and PPARs genes |
| title_short | Effects of active, inactive, and derivatives of Akkermansia muciniphila on the expression of the endocannabinoid system and PPARs genes |
| title_sort | effects of active inactive and derivatives of akkermansia muciniphila on the expression of the endocannabinoid system and ppars genes |
| url | https://doi.org/10.1038/s41598-022-13840-8 |
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