A metal tolerance protein, MTP10, is required for the calcium and magnesium homeostasis in Arabidopsis
Nutrient antagonism typically refers to the fact that too high a concentration of one nutrient inhibits the absorption of another nutrient. In plants, Ca2+ (Calcium) and Mg2+ (Magnesium) are the two most abundant divalent cations, which are known to have antagonistic interactions. Hence, maintaining...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2022-12-01
|
| Series: | Plant Signaling & Behavior |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/15592324.2021.2025322 |
| _version_ | 1797351976590114816 |
|---|---|
| author | Haiman Ge Qiaolin Shao Jinlin Chen Jiahong Chen Xueqin Li Yu Tan Wenzhi Lan Lei Yang Yuan Wang |
| author_facet | Haiman Ge Qiaolin Shao Jinlin Chen Jiahong Chen Xueqin Li Yu Tan Wenzhi Lan Lei Yang Yuan Wang |
| author_sort | Haiman Ge |
| collection | DOAJ |
| description | Nutrient antagonism typically refers to the fact that too high a concentration of one nutrient inhibits the absorption of another nutrient. In plants, Ca2+ (Calcium) and Mg2+ (Magnesium) are the two most abundant divalent cations, which are known to have antagonistic interactions. Hence, maintaining their homeostasis is crucial for plant growth and development. In this study, we showed that MTP10 (Metal Tolerance Protein 10) is an important regulator for maintaining homeostasis of Mg and Ca in Arabidopsis. The mtp10 mutant displayed severe growth retardation in the presence of excess Mg2+, to which the addition of Ca2+ was able to rescue the phenotype of mtp10 mutant. Additionally, the deficiency of Ca2+ in the culture medium accelerated the high-Mg sensitivity of the mtp10 mutant. The yeast complementation assay suggested that AtMTP10 had no Ca2+ transport activity. And the ICP-MS data further confirmed the antagonistic relationship between Ca2+ and Mg2+, with the addition of Ca2+ reducing the excessive accumulation of Mg2+ and high-Mg inhibiting the uptake of Ca2+. We conclude that the Arabidopsis MTP10 is essential for the regulation of Mg and Ca homeostasis. |
| first_indexed | 2024-03-08T13:08:31Z |
| format | Article |
| id | doaj.art-94d46c35862e44248b220221d2584937 |
| institution | Directory Open Access Journal |
| issn | 1559-2316 1559-2324 |
| language | English |
| last_indexed | 2024-03-08T13:08:31Z |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Plant Signaling & Behavior |
| spelling | doaj.art-94d46c35862e44248b220221d25849372024-01-18T15:58:21ZengTaylor & Francis GroupPlant Signaling & Behavior1559-23161559-23242022-12-0117110.1080/15592324.2021.20253222025322A metal tolerance protein, MTP10, is required for the calcium and magnesium homeostasis in ArabidopsisHaiman Ge0Qiaolin Shao1Jinlin Chen2Jiahong Chen3Xueqin Li4Yu Tan5Wenzhi Lan6Lei Yang7Yuan Wang8Nanjing UniversityCas Center for Excellence in Molecular Plant Sciences, Chinese Academy of SciencesNanjing UniversityCas Center for Excellence in Molecular Plant Sciences, Chinese Academy of SciencesCas Center for Excellence in Molecular Plant Sciences, Chinese Academy of SciencesCas Center for Excellence in Molecular Plant Sciences, Chinese Academy of SciencesNanjing UniversityNanjing UniversityNanjing UniversityNutrient antagonism typically refers to the fact that too high a concentration of one nutrient inhibits the absorption of another nutrient. In plants, Ca2+ (Calcium) and Mg2+ (Magnesium) are the two most abundant divalent cations, which are known to have antagonistic interactions. Hence, maintaining their homeostasis is crucial for plant growth and development. In this study, we showed that MTP10 (Metal Tolerance Protein 10) is an important regulator for maintaining homeostasis of Mg and Ca in Arabidopsis. The mtp10 mutant displayed severe growth retardation in the presence of excess Mg2+, to which the addition of Ca2+ was able to rescue the phenotype of mtp10 mutant. Additionally, the deficiency of Ca2+ in the culture medium accelerated the high-Mg sensitivity of the mtp10 mutant. The yeast complementation assay suggested that AtMTP10 had no Ca2+ transport activity. And the ICP-MS data further confirmed the antagonistic relationship between Ca2+ and Mg2+, with the addition of Ca2+ reducing the excessive accumulation of Mg2+ and high-Mg inhibiting the uptake of Ca2+. We conclude that the Arabidopsis MTP10 is essential for the regulation of Mg and Ca homeostasis.http://dx.doi.org/10.1080/15592324.2021.2025322mtp10mg homeostasiscalcium homeostasisarabidopsis |
| spellingShingle | Haiman Ge Qiaolin Shao Jinlin Chen Jiahong Chen Xueqin Li Yu Tan Wenzhi Lan Lei Yang Yuan Wang A metal tolerance protein, MTP10, is required for the calcium and magnesium homeostasis in Arabidopsis Plant Signaling & Behavior mtp10 mg homeostasis calcium homeostasis arabidopsis |
| title | A metal tolerance protein, MTP10, is required for the calcium and magnesium homeostasis in Arabidopsis |
| title_full | A metal tolerance protein, MTP10, is required for the calcium and magnesium homeostasis in Arabidopsis |
| title_fullStr | A metal tolerance protein, MTP10, is required for the calcium and magnesium homeostasis in Arabidopsis |
| title_full_unstemmed | A metal tolerance protein, MTP10, is required for the calcium and magnesium homeostasis in Arabidopsis |
| title_short | A metal tolerance protein, MTP10, is required for the calcium and magnesium homeostasis in Arabidopsis |
| title_sort | metal tolerance protein mtp10 is required for the calcium and magnesium homeostasis in arabidopsis |
| topic | mtp10 mg homeostasis calcium homeostasis arabidopsis |
| url | http://dx.doi.org/10.1080/15592324.2021.2025322 |
| work_keys_str_mv | AT haimange ametaltoleranceproteinmtp10isrequiredforthecalciumandmagnesiumhomeostasisinarabidopsis AT qiaolinshao ametaltoleranceproteinmtp10isrequiredforthecalciumandmagnesiumhomeostasisinarabidopsis AT jinlinchen ametaltoleranceproteinmtp10isrequiredforthecalciumandmagnesiumhomeostasisinarabidopsis AT jiahongchen ametaltoleranceproteinmtp10isrequiredforthecalciumandmagnesiumhomeostasisinarabidopsis AT xueqinli ametaltoleranceproteinmtp10isrequiredforthecalciumandmagnesiumhomeostasisinarabidopsis AT yutan ametaltoleranceproteinmtp10isrequiredforthecalciumandmagnesiumhomeostasisinarabidopsis AT wenzhilan ametaltoleranceproteinmtp10isrequiredforthecalciumandmagnesiumhomeostasisinarabidopsis AT leiyang ametaltoleranceproteinmtp10isrequiredforthecalciumandmagnesiumhomeostasisinarabidopsis AT yuanwang ametaltoleranceproteinmtp10isrequiredforthecalciumandmagnesiumhomeostasisinarabidopsis AT haimange metaltoleranceproteinmtp10isrequiredforthecalciumandmagnesiumhomeostasisinarabidopsis AT qiaolinshao metaltoleranceproteinmtp10isrequiredforthecalciumandmagnesiumhomeostasisinarabidopsis AT jinlinchen metaltoleranceproteinmtp10isrequiredforthecalciumandmagnesiumhomeostasisinarabidopsis AT jiahongchen metaltoleranceproteinmtp10isrequiredforthecalciumandmagnesiumhomeostasisinarabidopsis AT xueqinli metaltoleranceproteinmtp10isrequiredforthecalciumandmagnesiumhomeostasisinarabidopsis AT yutan metaltoleranceproteinmtp10isrequiredforthecalciumandmagnesiumhomeostasisinarabidopsis AT wenzhilan metaltoleranceproteinmtp10isrequiredforthecalciumandmagnesiumhomeostasisinarabidopsis AT leiyang metaltoleranceproteinmtp10isrequiredforthecalciumandmagnesiumhomeostasisinarabidopsis AT yuanwang metaltoleranceproteinmtp10isrequiredforthecalciumandmagnesiumhomeostasisinarabidopsis |