Tetraploid Induction with Leaf Morphology and Sunburn Variation in <i>Sorbus pohuashanensis</i> (Hance) Hedl
<i>Sorbus pohuashanensis</i> (Hance) Hedl. is an important forestry species valued for its ornamental, medicinal, and ecological properties. Polyploidy breeding is an important method of germplasm innovation; however, polyploidy induction and phenotypic variation caused by chromosome dou...
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MDPI AG
2023-08-01
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| Online Access: | https://www.mdpi.com/1999-4907/14/8/1589 |
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| author | Zeren Zhang Yan Zhang Zexin Di Ruili Zhang Yanjuan Mu Tao Sun Zhihui Tian Yizeng Lu Jian Zheng |
| author_facet | Zeren Zhang Yan Zhang Zexin Di Ruili Zhang Yanjuan Mu Tao Sun Zhihui Tian Yizeng Lu Jian Zheng |
| author_sort | Zeren Zhang |
| collection | DOAJ |
| description | <i>Sorbus pohuashanensis</i> (Hance) Hedl. is an important forestry species valued for its ornamental, medicinal, and ecological properties. Polyploidy breeding is an important method of germplasm innovation; however, polyploidy induction and phenotypic variation caused by chromosome doubling in <i>S. pohuashanensis</i> are poorly understood. In this study, <i>S. pohuashanensis</i> seeds were used to explore the effects of different colchicine concentrations, cold stratification times, and seeds from different sources on polyploidy induction. Ploidy levels of the regenerated plants were determined by flow cytometry. The results showed that the tetraploid induction effect of <i>S. pohuashanensis</i> seeds was significantly affected by colchicine concentration, and the highest tetraploid induction rate of 24.75% was achieved by immersion in 0.2% (<i>w</i>/<i>v</i>) colchicine for 48 h. After 2 years of induction, 77 tetraploid plants were obtained. Compared to diploids, tetraploid plants showed significant variations in plant height, leaf morphology (apical leaflet width, middle leaflet width), and diameter of the middle petiole. The stomatal size and chloroplast number increased with chromosome doubling whereas the stomatal number and density decreased. In addition, significant differences in the percentage of sunburn associated with ploidy changes were observed. This study provides a technique for tetraploid induction of <i>S. pohuashanensis</i> seeds, showing the variation in traits caused by polyploidization and the effect of chromosome doubling on sunburn resistance. Tetraploidy induction provides a new direction for <i>S. pohuashanensis</i> germplasm innovation. |
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| issn | 1999-4907 |
| language | English |
| last_indexed | 2024-03-10T23:56:27Z |
| publishDate | 2023-08-01 |
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| series | Forests |
| spelling | doaj.art-94d54576dc1845529b714386d55881752023-11-19T01:08:58ZengMDPI AGForests1999-49072023-08-01148158910.3390/f14081589Tetraploid Induction with Leaf Morphology and Sunburn Variation in <i>Sorbus pohuashanensis</i> (Hance) HedlZeren Zhang0Yan Zhang1Zexin Di2Ruili Zhang3Yanjuan Mu4Tao Sun5Zhihui Tian6Yizeng Lu7Jian Zheng8School of Architecture, Beijing University of Agriculture, Beijing 102206, ChinaSchool of Architecture, Beijing University of Agriculture, Beijing 102206, ChinaSchool of Architecture, Beijing University of Agriculture, Beijing 102206, ChinaSchool of Architecture, Beijing University of Agriculture, Beijing 102206, ChinaShandong Provincial Center of Forest and Grass Germplasm Resources, Jinan 250102, ChinaShandong Provincial Center of Forest and Grass Germplasm Resources, Jinan 250102, ChinaSchool of Architecture, Beijing University of Agriculture, Beijing 102206, ChinaShandong Provincial Center of Forest and Grass Germplasm Resources, Jinan 250102, ChinaSchool of Architecture, Beijing University of Agriculture, Beijing 102206, China<i>Sorbus pohuashanensis</i> (Hance) Hedl. is an important forestry species valued for its ornamental, medicinal, and ecological properties. Polyploidy breeding is an important method of germplasm innovation; however, polyploidy induction and phenotypic variation caused by chromosome doubling in <i>S. pohuashanensis</i> are poorly understood. In this study, <i>S. pohuashanensis</i> seeds were used to explore the effects of different colchicine concentrations, cold stratification times, and seeds from different sources on polyploidy induction. Ploidy levels of the regenerated plants were determined by flow cytometry. The results showed that the tetraploid induction effect of <i>S. pohuashanensis</i> seeds was significantly affected by colchicine concentration, and the highest tetraploid induction rate of 24.75% was achieved by immersion in 0.2% (<i>w</i>/<i>v</i>) colchicine for 48 h. After 2 years of induction, 77 tetraploid plants were obtained. Compared to diploids, tetraploid plants showed significant variations in plant height, leaf morphology (apical leaflet width, middle leaflet width), and diameter of the middle petiole. The stomatal size and chloroplast number increased with chromosome doubling whereas the stomatal number and density decreased. In addition, significant differences in the percentage of sunburn associated with ploidy changes were observed. This study provides a technique for tetraploid induction of <i>S. pohuashanensis</i> seeds, showing the variation in traits caused by polyploidization and the effect of chromosome doubling on sunburn resistance. Tetraploidy induction provides a new direction for <i>S. pohuashanensis</i> germplasm innovation.https://www.mdpi.com/1999-4907/14/8/1589polyploid inductiontetraploidleaf shapesunburn |
| spellingShingle | Zeren Zhang Yan Zhang Zexin Di Ruili Zhang Yanjuan Mu Tao Sun Zhihui Tian Yizeng Lu Jian Zheng Tetraploid Induction with Leaf Morphology and Sunburn Variation in <i>Sorbus pohuashanensis</i> (Hance) Hedl Forests polyploid induction tetraploid leaf shape sunburn |
| title | Tetraploid Induction with Leaf Morphology and Sunburn Variation in <i>Sorbus pohuashanensis</i> (Hance) Hedl |
| title_full | Tetraploid Induction with Leaf Morphology and Sunburn Variation in <i>Sorbus pohuashanensis</i> (Hance) Hedl |
| title_fullStr | Tetraploid Induction with Leaf Morphology and Sunburn Variation in <i>Sorbus pohuashanensis</i> (Hance) Hedl |
| title_full_unstemmed | Tetraploid Induction with Leaf Morphology and Sunburn Variation in <i>Sorbus pohuashanensis</i> (Hance) Hedl |
| title_short | Tetraploid Induction with Leaf Morphology and Sunburn Variation in <i>Sorbus pohuashanensis</i> (Hance) Hedl |
| title_sort | tetraploid induction with leaf morphology and sunburn variation in i sorbus pohuashanensis i hance hedl |
| topic | polyploid induction tetraploid leaf shape sunburn |
| url | https://www.mdpi.com/1999-4907/14/8/1589 |
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