Simultaneous Quantitative Determination of Residues of Pyriproxyfen and Its Metabolites in Tea and Tea Infusion Using Ultra-Performance Liquid Chromatography–Tandem Mass Spectrometry
A reliable, simultaneous residue-analysis method for pyriproxyfen and its five metabolites in fresh tea leaves, green tea, black tea, green-tea infusion and black-tea infusion was developed and validated. The samples were extracted with acetonitrile, purified using a modified QuEChERs (quick, easy,...
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| Format: | Article |
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MDPI AG
2022-08-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/12/8/1829 |
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| author | Hongxia Li Qing Zhong Xinru Wang Fengjian Luo Li Zhou Zongmao Chen Xinzhong Zhang |
| author_facet | Hongxia Li Qing Zhong Xinru Wang Fengjian Luo Li Zhou Zongmao Chen Xinzhong Zhang |
| author_sort | Hongxia Li |
| collection | DOAJ |
| description | A reliable, simultaneous residue-analysis method for pyriproxyfen and its five metabolites in fresh tea leaves, green tea, black tea, green-tea infusion and black-tea infusion was developed and validated. The samples were extracted with acetonitrile, purified using a modified QuEChERs (quick, easy, cheap, effective, rugged and safe) method and determined using ultra-performance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS). The method was successfully calibrated in the range of 0.005–2.50 mg/L with correlation coefficients (<i>r</i>) equal to or above 0.9957. The limits of detection (LODs) were less than 0.002 mg/L. The average spiked recoveries of pyriproxyfen and its metabolites at four levels were 71.2~102.9% with relative standard deviations (RSDs) of 0.3~14.4%. The limits of quantification (LOQs) in fresh tea leaves, tea and tea infusion were 0.002 mg/kg, 0.005 mg/kg and 0.0002 mg/L, respectively. This proposed method was feasible and was applied to analyze the residues of pyriproxyfen and its five metabolites on real fresh tea leaf samples. The results indicated that the half-life (<i>t</i><sub>1/2</sub>) of pyriproxyfen on fresh tea leaves was 2.48 d, and the five metabolites were detected on fresh tea leaves during field growth after application. |
| first_indexed | 2024-03-09T04:49:02Z |
| format | Article |
| id | doaj.art-94d60e4cd84e4815abac51410bf9ec1e |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-09T04:49:02Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-94d60e4cd84e4815abac51410bf9ec1e2023-12-03T13:12:02ZengMDPI AGAgronomy2073-43952022-08-01128182910.3390/agronomy12081829Simultaneous Quantitative Determination of Residues of Pyriproxyfen and Its Metabolites in Tea and Tea Infusion Using Ultra-Performance Liquid Chromatography–Tandem Mass SpectrometryHongxia Li0Qing Zhong1Xinru Wang2Fengjian Luo3Li Zhou4Zongmao Chen5Xinzhong Zhang6Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, ChinaTea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, ChinaTea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, ChinaTea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, ChinaTea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, ChinaTea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, ChinaTea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, ChinaA reliable, simultaneous residue-analysis method for pyriproxyfen and its five metabolites in fresh tea leaves, green tea, black tea, green-tea infusion and black-tea infusion was developed and validated. The samples were extracted with acetonitrile, purified using a modified QuEChERs (quick, easy, cheap, effective, rugged and safe) method and determined using ultra-performance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS). The method was successfully calibrated in the range of 0.005–2.50 mg/L with correlation coefficients (<i>r</i>) equal to or above 0.9957. The limits of detection (LODs) were less than 0.002 mg/L. The average spiked recoveries of pyriproxyfen and its metabolites at four levels were 71.2~102.9% with relative standard deviations (RSDs) of 0.3~14.4%. The limits of quantification (LOQs) in fresh tea leaves, tea and tea infusion were 0.002 mg/kg, 0.005 mg/kg and 0.0002 mg/L, respectively. This proposed method was feasible and was applied to analyze the residues of pyriproxyfen and its five metabolites on real fresh tea leaf samples. The results indicated that the half-life (<i>t</i><sub>1/2</sub>) of pyriproxyfen on fresh tea leaves was 2.48 d, and the five metabolites were detected on fresh tea leaves during field growth after application.https://www.mdpi.com/2073-4395/12/8/1829teapyriproxyfenmetaboliteresidue analysisUPLC-MS/MS |
| spellingShingle | Hongxia Li Qing Zhong Xinru Wang Fengjian Luo Li Zhou Zongmao Chen Xinzhong Zhang Simultaneous Quantitative Determination of Residues of Pyriproxyfen and Its Metabolites in Tea and Tea Infusion Using Ultra-Performance Liquid Chromatography–Tandem Mass Spectrometry Agronomy tea pyriproxyfen metabolite residue analysis UPLC-MS/MS |
| title | Simultaneous Quantitative Determination of Residues of Pyriproxyfen and Its Metabolites in Tea and Tea Infusion Using Ultra-Performance Liquid Chromatography–Tandem Mass Spectrometry |
| title_full | Simultaneous Quantitative Determination of Residues of Pyriproxyfen and Its Metabolites in Tea and Tea Infusion Using Ultra-Performance Liquid Chromatography–Tandem Mass Spectrometry |
| title_fullStr | Simultaneous Quantitative Determination of Residues of Pyriproxyfen and Its Metabolites in Tea and Tea Infusion Using Ultra-Performance Liquid Chromatography–Tandem Mass Spectrometry |
| title_full_unstemmed | Simultaneous Quantitative Determination of Residues of Pyriproxyfen and Its Metabolites in Tea and Tea Infusion Using Ultra-Performance Liquid Chromatography–Tandem Mass Spectrometry |
| title_short | Simultaneous Quantitative Determination of Residues of Pyriproxyfen and Its Metabolites in Tea and Tea Infusion Using Ultra-Performance Liquid Chromatography–Tandem Mass Spectrometry |
| title_sort | simultaneous quantitative determination of residues of pyriproxyfen and its metabolites in tea and tea infusion using ultra performance liquid chromatography tandem mass spectrometry |
| topic | tea pyriproxyfen metabolite residue analysis UPLC-MS/MS |
| url | https://www.mdpi.com/2073-4395/12/8/1829 |
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