Characterization, antioxidant and antitumor activities of phenolic compounds from Amomum villosum Lour.
Amomum villosum Lour. (A. villosum), known as Sharen in China, is widely used for culinary and medicinal purposes due to containing a diverse set of bioactive compounds. In this study, the optimum ethanol extraction process was optimized and the composition and biological activities (antioxidant and...
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Frontiers Media S.A.
2024-02-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2024.1327164/full |
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| author | Ming Zhang Xi-xiang Shuai Xi-xiang Shuai Zhi Wei Tao-tao Dai Chang-bin Wei Ya Li Jun-jun He Li-qing Du |
| author_facet | Ming Zhang Xi-xiang Shuai Xi-xiang Shuai Zhi Wei Tao-tao Dai Chang-bin Wei Ya Li Jun-jun He Li-qing Du |
| author_sort | Ming Zhang |
| collection | DOAJ |
| description | Amomum villosum Lour. (A. villosum), known as Sharen in China, is widely used for culinary and medicinal purposes due to containing a diverse set of bioactive compounds. In this study, the optimum ethanol extraction process was optimized and the composition and biological activities (antioxidant and antitumor) of five different fractions (dichloromethane, petroleum ether, ethyl acetate, n-butanol and H2O) extracted from the ethanol extract of A. villosum were investigated. The results showed that the optimal extraction conditions were extraction temperature 80°C, extraction time 120 min, ethanol concentration 40% and solid–liquid ratio 1:25 g/mL. Moreover, 35 bioactive compounds were successfully identified by UPLC-ESI-QTOF-MS/MS from five factions for the first time, including 12 phenolic acids and derivatives, 2 organic acids, 12 flavonoids and derivatives, 2 oxylipins and 7 proanthocyanidins. Among them, ethyl acetate fraction (Fr-EtOAc) exhibited the highest content of total phenolic (374.01 mg GAE/g DW) and flavonoid (93.11 mg RE/g DW), where vanillic acid, catechin, epicatechin and protocatechuic acid were the predominant phenolic compounds that accounting for 81.65% of the quantified bioactive compounds. In addition, Fr-EtOAc demonstrated excellent total antioxidant activity (IC50 of DPPH and ABTS assays were 0.23, 0.08 mg/mL, respectively, and FRAP assay was 322.91 mg VCE/100 g DW) and antitumor activity (1,000 μg/mL, 79.04% inhibition rate). The results could provide guidance for the industrial production and application of A. villosum. |
| first_indexed | 2024-03-08T05:33:09Z |
| format | Article |
| id | doaj.art-94d779a17afd4d9fa80cf4348684c3c7 |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-03-08T05:33:09Z |
| publishDate | 2024-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-94d779a17afd4d9fa80cf4348684c3c72024-02-06T04:23:50ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2024-02-011110.3389/fnut.2024.13271641327164Characterization, antioxidant and antitumor activities of phenolic compounds from Amomum villosum Lour.Ming Zhang0Xi-xiang Shuai1Xi-xiang Shuai2Zhi Wei3Tao-tao Dai4Chang-bin Wei5Ya Li6Jun-jun He7Li-qing Du8South Subtropical Crops Research Institute, China Academy of Tropical Agricultural Sciences, Key Laboratory of Hainan Province for Postharvest Physiology and Technology of Tropical Horticultural Products, Zhanjiang, ChinaSouth Subtropical Crops Research Institute, China Academy of Tropical Agricultural Sciences, Key Laboratory of Hainan Province for Postharvest Physiology and Technology of Tropical Horticultural Products, Zhanjiang, ChinaState Key Laboratory of Food Science and Resources, Nanchang University, Nanchang, ChinaState Key Laboratory of Food Science and Resources, Nanchang University, Nanchang, ChinaState Key Laboratory of Food Science and Resources, Nanchang University, Nanchang, ChinaSouth Subtropical Crops Research Institute, China Academy of Tropical Agricultural Sciences, Key Laboratory of Hainan Province for Postharvest Physiology and Technology of Tropical Horticultural Products, Zhanjiang, ChinaSouth Subtropical Crops Research Institute, China Academy of Tropical Agricultural Sciences, Key Laboratory of Hainan Province for Postharvest Physiology and Technology of Tropical Horticultural Products, Zhanjiang, ChinaZhanjiang Experimental Station, China Academy of Tropical Agricultural Sciences, Zhanjiang, ChinaSouth Subtropical Crops Research Institute, China Academy of Tropical Agricultural Sciences, Key Laboratory of Hainan Province for Postharvest Physiology and Technology of Tropical Horticultural Products, Zhanjiang, ChinaAmomum villosum Lour. (A. villosum), known as Sharen in China, is widely used for culinary and medicinal purposes due to containing a diverse set of bioactive compounds. In this study, the optimum ethanol extraction process was optimized and the composition and biological activities (antioxidant and antitumor) of five different fractions (dichloromethane, petroleum ether, ethyl acetate, n-butanol and H2O) extracted from the ethanol extract of A. villosum were investigated. The results showed that the optimal extraction conditions were extraction temperature 80°C, extraction time 120 min, ethanol concentration 40% and solid–liquid ratio 1:25 g/mL. Moreover, 35 bioactive compounds were successfully identified by UPLC-ESI-QTOF-MS/MS from five factions for the first time, including 12 phenolic acids and derivatives, 2 organic acids, 12 flavonoids and derivatives, 2 oxylipins and 7 proanthocyanidins. Among them, ethyl acetate fraction (Fr-EtOAc) exhibited the highest content of total phenolic (374.01 mg GAE/g DW) and flavonoid (93.11 mg RE/g DW), where vanillic acid, catechin, epicatechin and protocatechuic acid were the predominant phenolic compounds that accounting for 81.65% of the quantified bioactive compounds. In addition, Fr-EtOAc demonstrated excellent total antioxidant activity (IC50 of DPPH and ABTS assays were 0.23, 0.08 mg/mL, respectively, and FRAP assay was 322.91 mg VCE/100 g DW) and antitumor activity (1,000 μg/mL, 79.04% inhibition rate). The results could provide guidance for the industrial production and application of A. villosum.https://www.frontiersin.org/articles/10.3389/fnut.2024.1327164/fullAmomum villosum Lour.phenolic compoundsUPLC-ESI-QTOF-MS/MSantioxidantantitumor |
| spellingShingle | Ming Zhang Xi-xiang Shuai Xi-xiang Shuai Zhi Wei Tao-tao Dai Chang-bin Wei Ya Li Jun-jun He Li-qing Du Characterization, antioxidant and antitumor activities of phenolic compounds from Amomum villosum Lour. Frontiers in Nutrition Amomum villosum Lour. phenolic compounds UPLC-ESI-QTOF-MS/MS antioxidant antitumor |
| title | Characterization, antioxidant and antitumor activities of phenolic compounds from Amomum villosum Lour. |
| title_full | Characterization, antioxidant and antitumor activities of phenolic compounds from Amomum villosum Lour. |
| title_fullStr | Characterization, antioxidant and antitumor activities of phenolic compounds from Amomum villosum Lour. |
| title_full_unstemmed | Characterization, antioxidant and antitumor activities of phenolic compounds from Amomum villosum Lour. |
| title_short | Characterization, antioxidant and antitumor activities of phenolic compounds from Amomum villosum Lour. |
| title_sort | characterization antioxidant and antitumor activities of phenolic compounds from amomum villosum lour |
| topic | Amomum villosum Lour. phenolic compounds UPLC-ESI-QTOF-MS/MS antioxidant antitumor |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2024.1327164/full |
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