The Effects of Temperature and Growth Phase on the Lipidomes of Sulfolobus islandicus and Sulfolobus tokodaii
The functionality of the plasma membrane is essential for all organisms. Adaption to high growth temperatures imposes challenges and Bacteria, Eukarya, and Archaea have developed several mechanisms to cope with these. Hyperthermophilic archaea have earlier been shown to synthesize tetraether membran...
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MDPI AG
2015-08-01
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| Series: | Life |
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| Online Access: | http://www.mdpi.com/2075-1729/5/3/1539 |
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| author | Sara Munk Jensen Vinnie Lund Neesgaard Sandra Landbo Nedergaard Skjoldbjerg Martin Brandl Christer S. Ejsing Alexander H. Treusch |
| author_facet | Sara Munk Jensen Vinnie Lund Neesgaard Sandra Landbo Nedergaard Skjoldbjerg Martin Brandl Christer S. Ejsing Alexander H. Treusch |
| author_sort | Sara Munk Jensen |
| collection | DOAJ |
| description | The functionality of the plasma membrane is essential for all organisms. Adaption to high growth temperatures imposes challenges and Bacteria, Eukarya, and Archaea have developed several mechanisms to cope with these. Hyperthermophilic archaea have earlier been shown to synthesize tetraether membrane lipids with an increased number of cyclopentane moieties at higher growth temperatures. Here we used shotgun lipidomics to study this effect as well as the influence of growth phase on the lipidomes of Sulfolobus islandicus and Sulfolobus tokodaii for the first time. Both species were cultivated at three different temperatures, with samples withdrawn during lag, exponential, and stationary phases. Three abundant tetraether lipid classes and one diether lipid class were monitored. Beside the expected increase in the number of cyclopentane moieties with higher temperature in both archaea, we observed previously unreported changes in the average cyclization of the membrane lipids throughout growth. The average number of cyclopentane moieties showed a significant dip in exponential phase, an observation that might help to resolve the currently debated biosynthesis pathway of tetraether lipids. |
| first_indexed | 2024-12-10T07:43:34Z |
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| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-12-10T07:43:34Z |
| publishDate | 2015-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-94dce7c08a054e9da1cecdd713101a2d2022-12-22T01:57:14ZengMDPI AGLife2075-17292015-08-01531539156610.3390/life5031539life5031539The Effects of Temperature and Growth Phase on the Lipidomes of Sulfolobus islandicus and Sulfolobus tokodaiiSara Munk Jensen0Vinnie Lund Neesgaard1Sandra Landbo Nedergaard Skjoldbjerg2Martin Brandl3Christer S. Ejsing4Alexander H. Treusch5Department of Biology and Nordic Center for Earth Evolution, University of Southern Denmark, Odense 5230, DenmarkDepartment of Biology and Nordic Center for Earth Evolution, University of Southern Denmark, Odense 5230, DenmarkDepartment of Biology and Nordic Center for Earth Evolution, University of Southern Denmark, Odense 5230, DenmarkDepartment of Physics, Chemistry and Pharmacy, University of Southern Denmark, Odense 5230, DenmarkDepartment of Biochemistry and Molecular Biology, VILLUM Center for Bioanalytical Sciences, University of Southern Denmark, Odense 5230, DenmarkDepartment of Biology and Nordic Center for Earth Evolution, University of Southern Denmark, Odense 5230, DenmarkThe functionality of the plasma membrane is essential for all organisms. Adaption to high growth temperatures imposes challenges and Bacteria, Eukarya, and Archaea have developed several mechanisms to cope with these. Hyperthermophilic archaea have earlier been shown to synthesize tetraether membrane lipids with an increased number of cyclopentane moieties at higher growth temperatures. Here we used shotgun lipidomics to study this effect as well as the influence of growth phase on the lipidomes of Sulfolobus islandicus and Sulfolobus tokodaii for the first time. Both species were cultivated at three different temperatures, with samples withdrawn during lag, exponential, and stationary phases. Three abundant tetraether lipid classes and one diether lipid class were monitored. Beside the expected increase in the number of cyclopentane moieties with higher temperature in both archaea, we observed previously unreported changes in the average cyclization of the membrane lipids throughout growth. The average number of cyclopentane moieties showed a significant dip in exponential phase, an observation that might help to resolve the currently debated biosynthesis pathway of tetraether lipids.http://www.mdpi.com/2075-1729/5/3/1539archaeaSulfolobusS. islandicusS. tokodaiimembranetemperaturegrowthtetraether lipidmass spectrometrylipidomics |
| spellingShingle | Sara Munk Jensen Vinnie Lund Neesgaard Sandra Landbo Nedergaard Skjoldbjerg Martin Brandl Christer S. Ejsing Alexander H. Treusch The Effects of Temperature and Growth Phase on the Lipidomes of Sulfolobus islandicus and Sulfolobus tokodaii Life archaea Sulfolobus S. islandicus S. tokodaii membrane temperature growth tetraether lipid mass spectrometry lipidomics |
| title | The Effects of Temperature and Growth Phase on the Lipidomes of Sulfolobus islandicus and Sulfolobus tokodaii |
| title_full | The Effects of Temperature and Growth Phase on the Lipidomes of Sulfolobus islandicus and Sulfolobus tokodaii |
| title_fullStr | The Effects of Temperature and Growth Phase on the Lipidomes of Sulfolobus islandicus and Sulfolobus tokodaii |
| title_full_unstemmed | The Effects of Temperature and Growth Phase on the Lipidomes of Sulfolobus islandicus and Sulfolobus tokodaii |
| title_short | The Effects of Temperature and Growth Phase on the Lipidomes of Sulfolobus islandicus and Sulfolobus tokodaii |
| title_sort | effects of temperature and growth phase on the lipidomes of sulfolobus islandicus and sulfolobus tokodaii |
| topic | archaea Sulfolobus S. islandicus S. tokodaii membrane temperature growth tetraether lipid mass spectrometry lipidomics |
| url | http://www.mdpi.com/2075-1729/5/3/1539 |
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