Immune Responses Induced by pVAX/TgERK7 against Toxoplasma gondii Infection in BALB/c Mice
Background: Toxoplasma gondii can infect all the warm-blooded vertebrates and cause serious toxoplasmosis. Extracellular signal-regulated kinase 7 in T. gondii (TgERK7) mediated the proliferation of this parasite may be a potential vaccine candidate. Thus, immune responses induced by TgERK7 were inv...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2019-12-01
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| Series: | Iranian Journal of Parasitology |
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| Online Access: | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/2139 |
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| author | Hai-Ting GUO Zhong-Yuan LI Jin-Lei WANG Zhao-Yu GENG Xing-Quan ZHU |
| author_facet | Hai-Ting GUO Zhong-Yuan LI Jin-Lei WANG Zhao-Yu GENG Xing-Quan ZHU |
| author_sort | Hai-Ting GUO |
| collection | DOAJ |
| description | Background: Toxoplasma gondii can infect all the warm-blooded vertebrates and cause serious toxoplasmosis. Extracellular signal-regulated kinase 7 in T. gondii (TgERK7) mediated the proliferation of this parasite may be a potential vaccine candidate. Thus, immune responses induced by TgERK7 were investigated in this study using a DNA vaccine strategy.
Methods: pVAX/TgERK7 plasmid was constructed and used to immunize BALB/c mice for three times with two-week intervals. The challenge and the investigation of humoral and cellular immune responses were performed at two weeks post the last immunization, and the survival times of the infected mice were daily recorded until all of them were dead.
Results: The innate immune response with higher concentrations of IFN-γ, TNF-α, IL2 and IL12p70 in sera (P < 0.05), and the adaptive immune responses were evoked by the DNA immunizations, including specific antibody, lymphocyte proliferation, and the CD3e+CD4+ and CD3e+CD8a+ T cell-mediated response effects. Interestingly, no significant difference was detected in their survival times among all the experimental groups of mice that were challenged with GT1 tachyzoites or PRU cysts (P>0.05).
Conclusion: The successive immunizations with pVAX/TgERK7 can provoke the innate and adaptive immune responses of BALB/c mice, whereas the DNA vaccine-induced immunological efficacy is not sufficient for complete protection the host against T. gondii infection. |
| first_indexed | 2024-12-19T15:46:30Z |
| format | Article |
| id | doaj.art-94e18d09358346e8b3bd3a92f21e1ca8 |
| institution | Directory Open Access Journal |
| issn | 1735-7020 2008-238X |
| language | English |
| last_indexed | 2024-12-19T15:46:30Z |
| publishDate | 2019-12-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Parasitology |
| spelling | doaj.art-94e18d09358346e8b3bd3a92f21e1ca82022-12-21T20:15:19ZengTehran University of Medical SciencesIranian Journal of Parasitology1735-70202008-238X2019-12-0114410.18502/ijpa.v14i4.20972139Immune Responses Induced by pVAX/TgERK7 against Toxoplasma gondii Infection in BALB/c MiceHai-Ting GUO0Zhong-Yuan LI1Jin-Lei WANG2Zhao-Yu GENG3Xing-Quan ZHU4Guangxi Key Laboratory for Brain and Cognitive Neurosciences, Guilin Medical College, Guilin, China AND College of Biological Science and Technology, Heilongjiang Bayi Agricultural University, Daqing, ChinaGuangxi Key Laboratory for Brain and Cognitive Neurosciences, Guilin Medical College, Guilin, China AND State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, ChinaState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, ChinaCollege of Animal Science and Technology, Anhui Agricultural University, Hefei, ChinaState Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, ChinaBackground: Toxoplasma gondii can infect all the warm-blooded vertebrates and cause serious toxoplasmosis. Extracellular signal-regulated kinase 7 in T. gondii (TgERK7) mediated the proliferation of this parasite may be a potential vaccine candidate. Thus, immune responses induced by TgERK7 were investigated in this study using a DNA vaccine strategy. Methods: pVAX/TgERK7 plasmid was constructed and used to immunize BALB/c mice for three times with two-week intervals. The challenge and the investigation of humoral and cellular immune responses were performed at two weeks post the last immunization, and the survival times of the infected mice were daily recorded until all of them were dead. Results: The innate immune response with higher concentrations of IFN-γ, TNF-α, IL2 and IL12p70 in sera (P < 0.05), and the adaptive immune responses were evoked by the DNA immunizations, including specific antibody, lymphocyte proliferation, and the CD3e+CD4+ and CD3e+CD8a+ T cell-mediated response effects. Interestingly, no significant difference was detected in their survival times among all the experimental groups of mice that were challenged with GT1 tachyzoites or PRU cysts (P>0.05). Conclusion: The successive immunizations with pVAX/TgERK7 can provoke the innate and adaptive immune responses of BALB/c mice, whereas the DNA vaccine-induced immunological efficacy is not sufficient for complete protection the host against T. gondii infection.https://ijpa.tums.ac.ir/index.php/ijpa/article/view/2139Toxoplasma gondiiToxoplasmosisERK7DNA vaccineImmune response |
| spellingShingle | Hai-Ting GUO Zhong-Yuan LI Jin-Lei WANG Zhao-Yu GENG Xing-Quan ZHU Immune Responses Induced by pVAX/TgERK7 against Toxoplasma gondii Infection in BALB/c Mice Iranian Journal of Parasitology Toxoplasma gondii Toxoplasmosis ERK7 DNA vaccine Immune response |
| title | Immune Responses Induced by pVAX/TgERK7 against Toxoplasma gondii Infection in BALB/c Mice |
| title_full | Immune Responses Induced by pVAX/TgERK7 against Toxoplasma gondii Infection in BALB/c Mice |
| title_fullStr | Immune Responses Induced by pVAX/TgERK7 against Toxoplasma gondii Infection in BALB/c Mice |
| title_full_unstemmed | Immune Responses Induced by pVAX/TgERK7 against Toxoplasma gondii Infection in BALB/c Mice |
| title_short | Immune Responses Induced by pVAX/TgERK7 against Toxoplasma gondii Infection in BALB/c Mice |
| title_sort | immune responses induced by pvax tgerk7 against toxoplasma gondii infection in balb c mice |
| topic | Toxoplasma gondii Toxoplasmosis ERK7 DNA vaccine Immune response |
| url | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/2139 |
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