Maintenance of large subpopulations of differentiated CD8 T-cells two years after cytomegalovirus infection in Gambian infants.
In a previously published study, we found that large differentiated subpopulations of CD8 T-cells emerged rapidly after CMV infection in young infants and persisted throughout the following year. Here we describe a follow-up study conducted on the same infants to establish whether the differentiated...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2008-08-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC2483415?pdf=render |
| _version_ | 1818022926803271680 |
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| author | David J C Miles Marianne van der Sande David Jeffries Steve Kaye Olubukola Ojuola Mariama Sanneh Momodou Cox Melba S Palmero Ebrima S Touray Pauline Waight Sarah Rowland-Jones Hilton Whittle Arnaud Marchant |
| author_facet | David J C Miles Marianne van der Sande David Jeffries Steve Kaye Olubukola Ojuola Mariama Sanneh Momodou Cox Melba S Palmero Ebrima S Touray Pauline Waight Sarah Rowland-Jones Hilton Whittle Arnaud Marchant |
| author_sort | David J C Miles |
| collection | DOAJ |
| description | In a previously published study, we found that large differentiated subpopulations of CD8 T-cells emerged rapidly after CMV infection in young infants and persisted throughout the following year. Here we describe a follow-up study conducted on the same infants to establish whether the differentiated subpopulations continued through the second year post-infection.CMV-specific cells identified using tetramers remained more activated and differentiated than the overall CD8 population. The large subpopulation of differentiated cytotoxic (CD28(-)CD62L(-)Bcl-2(low)CD95(+)perforin(+)) cells that emerged rapidly after infection remained stable after two years. No similar subpopulation was found in CMV-uninfected infants indicating that two years after infection, CMV remained a major factor in driving CD8 T-cell differentiation. Although markers of activation (CD45R0 and HLA-D) declined throughout the first year, HLA-D expression continued to decline during the second year and CD45R0 expression increased slightly. The age-related increase in IFNgamma response observed during the first year continued but was non-significant during the second year, indicating that the rate of functional improvement had slowed substantially. CONCLUSIONS / SIGNIFICANCE: The large differentiated subpopulations of CD8 T-cells that had emerged immediately after CMV infection persisted through the second year post-infection, while levels of activation and functional capacity remained fairly constant. |
| first_indexed | 2024-12-10T03:36:11Z |
| format | Article |
| id | doaj.art-94e1dbf0e3ac4e15baea18b47e1aca42 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T03:36:11Z |
| publishDate | 2008-08-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-94e1dbf0e3ac4e15baea18b47e1aca422022-12-22T02:03:41ZengPublic Library of Science (PLoS)PLoS ONE1932-62032008-08-0138e290510.1371/journal.pone.0002905Maintenance of large subpopulations of differentiated CD8 T-cells two years after cytomegalovirus infection in Gambian infants.David J C MilesMarianne van der SandeDavid JeffriesSteve KayeOlubukola OjuolaMariama SannehMomodou CoxMelba S PalmeroEbrima S TourayPauline WaightSarah Rowland-JonesHilton WhittleArnaud MarchantIn a previously published study, we found that large differentiated subpopulations of CD8 T-cells emerged rapidly after CMV infection in young infants and persisted throughout the following year. Here we describe a follow-up study conducted on the same infants to establish whether the differentiated subpopulations continued through the second year post-infection.CMV-specific cells identified using tetramers remained more activated and differentiated than the overall CD8 population. The large subpopulation of differentiated cytotoxic (CD28(-)CD62L(-)Bcl-2(low)CD95(+)perforin(+)) cells that emerged rapidly after infection remained stable after two years. No similar subpopulation was found in CMV-uninfected infants indicating that two years after infection, CMV remained a major factor in driving CD8 T-cell differentiation. Although markers of activation (CD45R0 and HLA-D) declined throughout the first year, HLA-D expression continued to decline during the second year and CD45R0 expression increased slightly. The age-related increase in IFNgamma response observed during the first year continued but was non-significant during the second year, indicating that the rate of functional improvement had slowed substantially. CONCLUSIONS / SIGNIFICANCE: The large differentiated subpopulations of CD8 T-cells that had emerged immediately after CMV infection persisted through the second year post-infection, while levels of activation and functional capacity remained fairly constant.http://europepmc.org/articles/PMC2483415?pdf=render |
| spellingShingle | David J C Miles Marianne van der Sande David Jeffries Steve Kaye Olubukola Ojuola Mariama Sanneh Momodou Cox Melba S Palmero Ebrima S Touray Pauline Waight Sarah Rowland-Jones Hilton Whittle Arnaud Marchant Maintenance of large subpopulations of differentiated CD8 T-cells two years after cytomegalovirus infection in Gambian infants. PLoS ONE |
| title | Maintenance of large subpopulations of differentiated CD8 T-cells two years after cytomegalovirus infection in Gambian infants. |
| title_full | Maintenance of large subpopulations of differentiated CD8 T-cells two years after cytomegalovirus infection in Gambian infants. |
| title_fullStr | Maintenance of large subpopulations of differentiated CD8 T-cells two years after cytomegalovirus infection in Gambian infants. |
| title_full_unstemmed | Maintenance of large subpopulations of differentiated CD8 T-cells two years after cytomegalovirus infection in Gambian infants. |
| title_short | Maintenance of large subpopulations of differentiated CD8 T-cells two years after cytomegalovirus infection in Gambian infants. |
| title_sort | maintenance of large subpopulations of differentiated cd8 t cells two years after cytomegalovirus infection in gambian infants |
| url | http://europepmc.org/articles/PMC2483415?pdf=render |
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