In vitro Multiple Shoot Regeneration from Petunia hybrida
An efficient plant regeneration protocol was developed from in vitro germinated seeds of Petunia hybrida an ornamentally important plant in the family Solanaceae. Shoot tip and node explants of Petunia hybrida were cultured on MS basal medium supplemented with different concentrations and combinatio...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Turkish Science and Technology Publishing (TURSTEP)
2019-10-01
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| Series: | Turkish Journal of Agriculture: Food Science and Technology |
| Subjects: | |
| Online Access: | http://www.agrifoodscience.com/index.php/TURJAF/article/view/2570 |
| _version_ | 1797917946087997440 |
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| author | Rebaz Rasul Habas Musa Turker Fethi Ahmet Ozdemir |
| author_facet | Rebaz Rasul Habas Musa Turker Fethi Ahmet Ozdemir |
| author_sort | Rebaz Rasul Habas |
| collection | DOAJ |
| description | An efficient plant regeneration protocol was developed from in vitro germinated seeds of Petunia hybrida an ornamentally important plant in the family Solanaceae. Shoot tip and node explants of Petunia hybrida were cultured on MS basal medium supplemented with different concentrations and combinations of Benzyl amino purine (BAP), 1-Naphthaleneacetic acid (NAA), Indole-3-butyric acid (IBA) and Gibberellic acid (GA3). The highest shoot length was obtained from MS medium supplemented with 1 mg/l BAP + 1 mg/l NAA. The highest shoot number (3 shoots/explant) were obtained from MS medium supplemented with 0.6 mg/l BAP + 0.5 mg/l IBA. The isolated shoots were transferred to MS basal medium supplemented with different concentrations of GA3 ranging from 0.05, 0.2, 0.5 and 1 mg/l for shoot elongation. The highest shoot length (5.75 cm) was recorded from the MS medium supplemented with 0.2 mg/l GA3 +0.2 mg/l BAP. Rooting of regenerated shoots were achieved on MS medium supplemented with 0.1-1 mg/1 IBA and NAA. The regenerated shoots with well developed roots were successfully acclimatized and established in pots containing sterilized peat moss and grown under laboratory conditions with 70% survival rates. |
| first_indexed | 2024-04-10T13:21:34Z |
| format | Article |
| id | doaj.art-94e3353340124325be15902f67cdff2d |
| institution | Directory Open Access Journal |
| issn | 2148-127X |
| language | English |
| last_indexed | 2024-04-10T13:21:34Z |
| publishDate | 2019-10-01 |
| publisher | Turkish Science and Technology Publishing (TURSTEP) |
| record_format | Article |
| series | Turkish Journal of Agriculture: Food Science and Technology |
| spelling | doaj.art-94e3353340124325be15902f67cdff2d2023-02-15T16:12:02ZengTurkish Science and Technology Publishing (TURSTEP)Turkish Journal of Agriculture: Food Science and Technology2148-127X2019-10-017101554156010.24925/turjaf.v7i10.1554-1560.25701267In vitro Multiple Shoot Regeneration from Petunia hybridaRebaz Rasul Habas0Musa Turker1Fethi Ahmet Ozdemir2Department of Molecular Biology and Genetics, Institute of Natural and Applied Science, Van Yüzüncü Yıl Universtiy, 65080 VanDepartment of Bioengineering, Faculty of Chemical and Metallurgical Engineering, Yıldız Technical University, 34210 IstanbulDepartment of Molecular Biology and Genetics, Faculty of Science and Art, Bingol University, 12000 BingolAn efficient plant regeneration protocol was developed from in vitro germinated seeds of Petunia hybrida an ornamentally important plant in the family Solanaceae. Shoot tip and node explants of Petunia hybrida were cultured on MS basal medium supplemented with different concentrations and combinations of Benzyl amino purine (BAP), 1-Naphthaleneacetic acid (NAA), Indole-3-butyric acid (IBA) and Gibberellic acid (GA3). The highest shoot length was obtained from MS medium supplemented with 1 mg/l BAP + 1 mg/l NAA. The highest shoot number (3 shoots/explant) were obtained from MS medium supplemented with 0.6 mg/l BAP + 0.5 mg/l IBA. The isolated shoots were transferred to MS basal medium supplemented with different concentrations of GA3 ranging from 0.05, 0.2, 0.5 and 1 mg/l for shoot elongation. The highest shoot length (5.75 cm) was recorded from the MS medium supplemented with 0.2 mg/l GA3 +0.2 mg/l BAP. Rooting of regenerated shoots were achieved on MS medium supplemented with 0.1-1 mg/1 IBA and NAA. The regenerated shoots with well developed roots were successfully acclimatized and established in pots containing sterilized peat moss and grown under laboratory conditions with 70% survival rates.http://www.agrifoodscience.com/index.php/TURJAF/article/view/2570petunia hybridain vitromultiple shootregenerationgibberellic acid |
| spellingShingle | Rebaz Rasul Habas Musa Turker Fethi Ahmet Ozdemir In vitro Multiple Shoot Regeneration from Petunia hybrida Turkish Journal of Agriculture: Food Science and Technology petunia hybrida in vitro multiple shoot regeneration gibberellic acid |
| title | In vitro Multiple Shoot Regeneration from Petunia hybrida |
| title_full | In vitro Multiple Shoot Regeneration from Petunia hybrida |
| title_fullStr | In vitro Multiple Shoot Regeneration from Petunia hybrida |
| title_full_unstemmed | In vitro Multiple Shoot Regeneration from Petunia hybrida |
| title_short | In vitro Multiple Shoot Regeneration from Petunia hybrida |
| title_sort | in vitro multiple shoot regeneration from petunia hybrida |
| topic | petunia hybrida in vitro multiple shoot regeneration gibberellic acid |
| url | http://www.agrifoodscience.com/index.php/TURJAF/article/view/2570 |
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